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Invisiblemushhead
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Re: Some notes about LCs [Re: sonoramo]
    #27054115 - 11/23/20 02:57 PM (3 years, 2 months ago)

Quote:

sonoramo said:
Quote:

starbones said:
Oh I meant sterilizing the syringe WITH LC solution inside of it in the PC.
...
Instead of fill, use, change needle (I use a fresh 18g everytime I go into my LC quarts, cheap enough to not do the flaming) then fill, use, change needle, fill, use, blah.

I guess I could sterilize a bunch, make a new slave quart and fill them all prior to inoculation day.




Are you thinking to grow mycelium inside the syringe? I'd worry about clogging the needle. Even after spinning a bar inside an LC, mycelium clumps often block the tip of my 16ga needles as I'm drawing into the syringe. Not a huge problem because I can push it back out and try again. If that happens with a clump that's growing inside the syringe, there won't be any backing out.



Shake it like you're giving your worst enemy an unopened Sprite.


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OfflineA.k.aM
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Re: Some notes about LCs [Re: mushhead]
    #27054134 - 11/23/20 03:09 PM (3 years, 2 months ago)

Verum said that happens with his lc gun too. Gotta keep it flushed out or the myc grows inside the tubing and jams things up.


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Offlinestarbones
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Registered: 03/04/20
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Re: Some notes about LCs [Re: A.k.a]
    #27054204 - 11/23/20 04:07 PM (3 years, 2 months ago)

I can see that happening easy.

I just dumped a quart of LC today that was acting odd and consolidating into large clumps. It had begun to gel to the side of the jars and around the top, myc strands growing.

I have other jars of the same LC and they're not doing that so I assumed it was stressed and trying to fruit because of contamination. The blobs that came out were not something I could see passing through a 18g.

Only way I could see it working is if the syringe was sat vertically so the myc colony would be kept down to the plunger away from the needle.

10 points to gryffindor for anyone who gets a bit of LC in a syringe to pin.


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Heavy metal ballads out the guitar where lions run.




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Invisiblemushhead
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Re: Some notes about LCs [Re: starbones]
    #27054270 - 11/23/20 04:55 PM (3 years, 2 months ago)

why does gryffindor get all the points?

EDIT:
Quote:

mushhead said:
I've been working on my LC nute levels. Trying to get them as clear as everyone's here.
My LCs are clean, but have sediment because I've been experimenting with nute levels.
My first batch is 5g LME to 500ml distilled water. LOTS of sediment. 
Second is 2.5g LME to 500ml distilled water. Medium-minor sediment.
Third is 1g LME to 500ml distilled water. Mostly none. Small amount visible on bottom of jar.
Fourth is .5g LME to 500ml distilled water. Pretty clear.
Pics after I finish a bunch of errands today.





Edited by mushhead (11/23/20 07:30 PM)


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InvisibleCulliganman360
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Re: Some notes about LCs [Re: mushhead]
    #27058948 - 11/26/20 03:52 PM (3 years, 2 months ago)

Put a wedge in these Monday around 2-3pm so about 50 hours since inoculation.

Diluted 1 part GW 20 parts water


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OfflineBrownBear
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Re: Some notes about LCs [Re: Culliganman360]
    #27060350 - 11/27/20 02:52 PM (3 years, 2 months ago)

My 250 ml lc's with 3 pinches of ny ended up yielding me 164 ml's of dense liquid inoculant. I was only able to pull out 60 ml's of water but even that had some mycelium in it. That left about 26 ml's of lc that I couldn't actually extract from the jar. The next batch was made with 200 ml of water and 4 pinches of ny. I'm hoping to not have to pull any water out of them and have a maximum dense liquid culture. So far I believe that my idea is working as planned and cannot wait for the next batch to finish up.



Edited by BrownBear (11/27/20 03:18 PM)


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InvisibleCulliganman360
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Re: Some notes about LCs [Re: BrownBear]
    #27062965 - 11/29/20 12:38 PM (3 years, 1 month ago)

How many ml of lc should I put in a 5qt spawn bag?


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OfflineMLPismyOPSEC
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Re: Some notes about LCs [Re: Culliganman360]
    #27063614 - 11/29/20 07:21 PM (3 years, 1 month ago)

I do 10-15 mL per quart, jars or bags.


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: MLPismyOPSEC]
    #27069120 - 12/02/20 11:01 PM (3 years, 1 month ago)

Blue Helix, I’ve got a question for you. When you make your substrate bags do you find the dry grain hydrates during your PC cycle and pulls some moisture out? I just made a couple 13.5lb bags consisting of hydrated coir, dry oats, wheat bran, vermiculite and water. They were just a tad wet going into the PC, but I’m hoping because I used dry grain it will hydrate during the cook and dry out the rest of the mix enough to accommodate the LC. I plan to inoculate them with ~140ml of LC.


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflinePitcherCrab
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Re: Some notes about LCs [Re: PitcherCrab]
    #27070424 - 12/03/20 05:40 PM (3 years, 1 month ago)

Quote:

PitcherCrab said:
Blue Helix, I’ve got a question for you. When you make your substrate bags do you find the dry grain hydrates during your PC cycle and pulls some moisture out? I just made a couple 13.5lb bags consisting of hydrated coir, dry oats, wheat bran, vermiculite and water. They were just a tad wet going into the PC, but I’m hoping because I used dry grain it will hydrate during the cook and dry out the rest of the mix enough to accommodate the LC. I plan to inoculate them with ~140ml of LC.




Nevermind, I answered my own question. The oats hydrated and took the moisture from slightly too wet to perfect. Just knocked them up with ~140ml of LC. :grin: I'm excited to see how they go. They're some really hefty substrate bags.


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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinekanemush
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Re: Some notes about LCs [Re: PitcherCrab]
    #27075362 - 12/06/20 02:04 PM (3 years, 1 month ago)

this is the 2nd broth I have made as the first did not go well. I noced them up about 3 days ago and started spinning the jars 1 day ago.



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InvisibleMateja
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Re: Some notes about LCs [Re: kanemush]
    #27075369 - 12/06/20 02:07 PM (3 years, 1 month ago)

How did you make that broth it appears turbid already and what's with the thick (foam?) layer on top?


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Offlinekanemush
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Re: Some notes about LCs [Re: Mateja]
    #27075380 - 12/06/20 02:12 PM (3 years, 1 month ago)

i just stirred it before I took the pic so that is the reason for the foam on top. I think it is possible turbid also I used 250ml water and 1g of lme.


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InvisibleMateja
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Re: Some notes about LCs [Re: kanemush]
    #27075396 - 12/06/20 02:25 PM (3 years, 1 month ago)

You know you could dilute that down to 1/80 and still have a broth that generates healthy fast growing mycelium only difference is you end up with a crystal clear broth that doesn't foam, doesn't contain particles thus is extremely easy to inspect. 4% Nutrients is too much even for agar.


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Offlinekanemush
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Re: Some notes about LCs [Re: Mateja]
    #27075400 - 12/06/20 02:27 PM (3 years, 1 month ago)

thank you for the suggestions seems like i will be making some new broths then.


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InvisibleMateja
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Re: Some notes about LCs [Re: kanemush]
    #27075403 - 12/06/20 02:28 PM (3 years, 1 month ago)

Making broths is too easy, gl man :thumbup:


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Offlinebongoman
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Re: Some notes about LCs [Re: Mateja]
    #27075463 - 12/06/20 02:59 PM (3 years, 1 month ago)

I’ve been using media bottles for my LC’s and pouring them to grain jars.

I’m curious about aspirating into syringes but aren’t sure of the best way to proceed here.

Should I only be aspirating through a SHIP or can I aspirate directly from an open media bottle of LC or is this too risky?

Also, for long term storage, how does one actually transfer LC to a vacutainer?


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InvisibleLemgrub
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Re: Some notes about LCs [Re: bongoman]
    #27076097 - 12/06/20 08:24 PM (3 years, 1 month ago)

0.4%. Should be a fine concentration. The broth does look a bit turbid tho


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OfflinePitcherCrab
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Re: Some notes about LCs [Re: Lemgrub]
    #27077455 - 12/07/20 05:30 PM (3 years, 1 month ago)

I've got some really nice LCs here. These are 2 of 5 varieties. All have really nice growth, I put them on the stir plate every couple days to break them up. I inoculated them with just a tiny bit of mycelium from a petri using a sterile swab. I just knocked up x2 13.5lb substrate bags with the RW... excited to see how it goes.



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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Offlinenmd_myco
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Re: Some notes about LCs [Re: bongoman]
    #27078598 - 12/08/20 11:08 AM (3 years, 1 month ago)

Quote:

bongoman said:


Also, for long term storage, how does one actually transfer LC to a vacutainer?




with a syringe. The vacutainer will literally suck 9-10ml from the syringe into it.


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