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10timeshigh
:D

Registered: 10/03/17
Posts: 10
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Agar and first time grow strategies
#26976145 - 10/08/20 09:02 PM (3 years, 3 months ago) |
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Hey so I got some iso/cloning questions regarding starting from absolute scratch. As well as a little bonus thing to do with agar medium.
If I'm a perfectionist and am looking to get perfect flushes just for the sake of it, am I correct in taking this action? MS to agar, 2-3 transfers of early healthy mycelium growth (not looking for rhizomorphic because doesn't matter for first grow plus saw RR say he transfers early growth) then put that limited MS to grain and fruit with shoe box tek or something. All the while isolating the original agar transfers out to the best rhizomorphic monocultures. Then clone the fastest growing cluster from the first grow. With that clone make 1 transfer of super early mycelium so as to be contam safe as well as keeping as much of that clone's make up together. Also grow out a monoculture from the best rhizomorphic growth from the clone. Then with lets say 5 monocultures from the original MS you also have one clone culture and one monoculture derived from the clone. So you shoe box tek all those to see which is best.
Please leave tips or suggestions. Like how many plates of which I should make at each stage.
Is a monoculture in the end better than a clone.
As for the agar medium bonus question: Does anyone know of anyone who has used xylitol for the sugar portion of agar. Like it has less calories than dextrose is present in trees in nature and I think its anti bacterial or something so it sounds perfect for fast growing mycelium. Only reason is cuz I randomly have some and am not sure if I have any honey.
Thank youuuu
Edited by 10timeshigh (10/08/20 09:38 PM)
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Forgotten Oak
Stranger

Registered: 11/09/19
Posts: 32
Last seen: 2 years, 11 months
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Re: Agar and first time grow strategies [Re: 10timeshigh]
#26976186 - 10/08/20 09:35 PM (3 years, 3 months ago) |
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Quote:
10timeshigh said:
As for the agar medium bonus question: Does anyone know of anyone who has used zylitil for the sugar portion of agar. Like it has less calories than dextrose is present in trees in nature and I think its anti bacterial or something so it sounds perfect for fast growing mycelium. Only reason is cuz I randomly have some and am not sure if I have any honey.
I'm very new to all of this myself,so take what I say with a big grain of salt.
I've never heard of zylitil, if it is the same thing as xylitol, the sugar substitute (which fits your description of it) then it has some anti-fungal properties. Obviously, this would pose a big problem if you were to use it in your growth medium. Kind of like mixing Round-Up in with your lawn fertilizer.
(PDF Warning)
https://pdfs.semanticscholar.org/4c8c/737c79282f80e2759a191987e0722b238910.pdf
Quote:
CONCLUSION
According to the results of this survey, xylitol has considerable antimicrobial effects. It can decrease the adherence of C. albicans to buccal epithelial cells and weaken this microorganism against our immune system by making it more susceptible to lysozyme. The growth of this fungus is also inhibited in the presence of xylitol
Beside that fact, since it's not actually a sugar, I kind of doubt it would offer the mycelium much if any nutrition to speak of. I would definitely not use it myself, assuming that what you're referring to as zylitil is known to me as xylitol. I googled zylitil and couldn't find anything.
Edited by Forgotten Oak (10/08/20 09:37 PM)
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10timeshigh
:D

Registered: 10/03/17
Posts: 10
Last seen: 3 years, 3 months
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Re: Agar and first time grow strategies [Re: Forgotten Oak]
#26976193 - 10/08/20 09:41 PM (3 years, 3 months ago) |
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totally meant xylitol, must of spell corrected it wrong. Is it really anti fungal? Also thought it is a type of sugar. Maybe I'll test it as a side project
Edited by 10timeshigh (10/08/20 09:45 PM)
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Forgotten Oak
Stranger

Registered: 11/09/19
Posts: 32
Last seen: 2 years, 11 months
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Re: Agar and first time grow strategies [Re: 10timeshigh]
#26976234 - 10/08/20 10:11 PM (3 years, 3 months ago) |
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Interestingly, this question of xylitol in agar has come up before, although the OP never followed up to indicate whether they ever tried it, or what the result were. But there's a little bit of interesting information in the thread in case you're interested:
https://www.shroomery.org/forums/showflat.php/Number/23829748
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10timeshigh
:D

Registered: 10/03/17
Posts: 10
Last seen: 3 years, 3 months
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Re: Agar and first time grow strategies [Re: Forgotten Oak]
#26976256 - 10/08/20 10:38 PM (3 years, 3 months ago) |
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I saw that but I need resultss  
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: Agar and first time grow strategies [Re: 10timeshigh]
#26976290 - 10/08/20 11:32 PM (3 years, 3 months ago) |
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Like Bod said in the similar thread you posted in 2017; forget monocultures for now and focus on clean growth. It will at least start you down that road and you'll grow mushrooms in the meantime. Gotta walk before you can run.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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10timeshigh
:D

Registered: 10/03/17
Posts: 10
Last seen: 3 years, 3 months
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Re: Agar and first time grow strategies [Re: Sir Pentinite]
#26976344 - 10/09/20 12:46 AM (3 years, 3 months ago) |
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I can dig it, but I'm still gonna need a plan at some point. As I mentioned in my paragraph I plan to work with agar while I grow. I'm looking for a MS start to monoculture/clone strategy.
I come from a print making background which is very meticulous and unforgiving and don't see my self making many mistakes so I was curious if any masters out there had a maximum efficiency strategy for this.
None of my perfectionism will take away from love of the game. I look forward to just growing and getting my feet wet. But i'd like to create a monoculture too. 
I'm also looking for tips and stuff regarding this kinda thing. For example I don't understand why RR said he takes early mycelium growth when isolating. Rather than what I usually read which is strong rhizomorphic growt.
Edited by 10timeshigh (10/09/20 12:48 AM)
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