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werd618
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Trying to get potency consistency with agar
#26975889 - 10/08/20 06:01 PM (3 years, 3 months ago) |
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So I had a batch that seemed to be particularly potent that started from a spore syringe. I took a piece of the biggest shroom in the tub and placed it in a ziplock as describe Cloneufc's easy Cloning tek. https://www.shroomery.org/forums/showflat.php/Number/12032286
Now I have a puff ball of mycelium. Tomorrow i'm going to take some of this mycelium and put in on an agar plate. Once it grows out i'll transfer it to 5 new plates then use those plates to inoculate 5 grain jars. Each one of those grain jars i'm going to transfer to 6 jars, so in the end i'll have a total of 30 jars.
Following this plan, I should have similar results from all my batches in terms of speed of growth, size, and potency, right? or am I missing something?
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Drummer1
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Re: Trying to get potency consistency with agar [Re: werd618]
#26975931 - 10/08/20 06:42 PM (3 years, 3 months ago) |
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.when I isolate I have a few different types of mushrooms and then I do more of an isolation and then they become more formatted.that's just from one mushroom.so no you will have to pick more clones from the batch you just cloned.does that makes since rofl?
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Mateja


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Re: Trying to get potency consistency with agar [Re: werd618]
#26975974 - 10/08/20 07:08 PM (3 years, 3 months ago) |
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Quote:
werd618 said: Following this plan, I should have similar results from all my batches in terms of speed of growth, size, and potency, right? or am I missing something?
Short answer? Yes, you will have similar characteristics and growth pattern.
-------------------- Cakes inside Water Tub
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Drummer1
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Re: Trying to get potency consistency with agar [Re: Mateja]
#26976034 - 10/08/20 07:48 PM (3 years, 3 months ago) |
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Quote:
Mateah said:
Quote:
werd618 said: Following this plan, I should have similar results from all my batches in terms of speed of growth, size, and potency, right? or am I missing something?
Short answer? Yes, you will have similar characteristics and growth pattern. 
Really ,I have them all over the place ,some trays do great others don't.one will be really fast the other will not.from one clone.if I take that clone and fruit it out a few more time and take from a cluster and do so a couple times ,then they will be the same,speed potency and overall pretty much the same.never have i had it from one cut.maybe it's just me rofl
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werd618
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Re: Trying to get potency consistency with agar [Re: Drummer1]
#26976080 - 10/08/20 08:12 PM (3 years, 3 months ago) |
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Drummer... So your saying you make a clone on agar, transfer that to grain and fruit it out. Take a clone from that fruit then repeat the process until things are consistent? How many times can you do this before you start to have genetic deterioration? Or does that "reset" every time you make a clone?
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A.k.a
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Re: Trying to get potency consistency with agar [Re: werd618]
#26976125 - 10/08/20 08:44 PM (3 years, 3 months ago) |
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Ime taking a clone sample and transferring it a couple times makes it way more likely to be consistent. I’ve run a bunch of clones right from the tissue and they come out similar to spore grows usually.
The ones I’ve transferred a few times are where I see tubs of similar looking mushrooms that mature at the same time.
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LAGM2020     
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werd618
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Re: Trying to get potency consistency with agar [Re: A.k.a]
#26976535 - 10/09/20 06:53 AM (3 years, 3 months ago) |
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When you make transfers, don't you run into senescence rather quickly? I keep finding conflicting statements about exactly how many generations you can have before you see senescence but the one I see the most is 3 generations. So.... I have my clone from the ziplock (generation 1), I transfer that to a master plate (generation 2), I make a transfer (generation 3), make another transfer (generation 4), split that plate into 5 plates (generation 5), inoculate 5 jars with wedges (generation 6), transfer each jar to 6 more jars (generation 7). So by the end i'm at generation 7. By this point aren't my genetics way different from the clone that I started with?
Also, if you have to make a bunch of transfers to achieve consistency, how is a clone or a master plate even helpful? Say I end up with 4 different isolates from one master plate. I fruit these out, some of them suck, some are good. Now that I have identified the isolate, I could transfer it to a bunch of plates but that would just take me one step closer, if not already into senescence. A year down the road I want to use the same master plate.... I would then have to repeat the process of transfers, fruiting out, and testing for potency, right?
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Roger Clemency
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Re: Trying to get potency consistency with agar [Re: werd618]
#26976589 - 10/09/20 07:56 AM (3 years, 3 months ago) |
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There are multiple strains in a single mushroom, so you clone it and if you transfer just once and go to grain you’ll still have some differences.
If you instead transfer a few wedges, then transfer from those 2-3 more times you will separate out the strains that were in that original mushroom. Or greatly reduce the number of strains you have on each plate. Then you run each of those to see which you like best.
If you find one you do like then you save it, and you come back later as you need to to grab a tiny piece of myc to expand out.
Senecense I don’t know much about but I don’t think it matters how many transfers you make I think that would kick in if you kept cloning a clone then cloning from that clone etc instead of doing the grow and then returning back to plates that hold the culture.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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A.k.a
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Re: Trying to get potency consistency with agar [Re: Roger Clemency]
#26976752 - 10/09/20 09:49 AM (3 years, 3 months ago) |
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AFAIK senescence isn’t something you gotta worry about as long as you’re not planning on g2g the same jars 50 times.
Im pretty sure plate transfers take very little toll on the myc. I’ve seen a bunch of people fruiting t10 and higher plates no problem.
Verum did that isolate thread and I think those were over 100 transfers deep.
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LAGM2020     
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werd618
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Re: Trying to get potency consistency with agar [Re: A.k.a]
#26977068 - 10/09/20 01:18 PM (3 years, 3 months ago) |
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Ok cool. That makes me feel a lot better. As always, thanks for the replies guys.
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Funky Monkey
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Re: Trying to get potency consistency with agar [Re: Roger Clemency]
#26977110 - 10/09/20 01:56 PM (3 years, 3 months ago) |
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Hey when did you start cultivating Roger?
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Roger Clemency
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Re: Trying to get potency consistency with agar [Re: Funky Monkey]
#26977291 - 10/09/20 04:36 PM (3 years, 3 months ago) |
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Back in twenty oh five. But then not again until like 2013, that’s when my career really took off and got me HOF status gangsta. Batter for the Indians.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Funky Monkey
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Re: Trying to get potency consistency with agar [Re: Roger Clemency]
#26977808 - 10/10/20 12:29 AM (3 years, 3 months ago) |
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Yeah, I saw your register date and was like no way. New account. Too good. 😅
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Roger Clemency
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Re: Trying to get potency consistency with agar [Re: Funky Monkey]
#26978303 - 10/10/20 10:33 AM (3 years, 3 months ago) |
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I forgot my damn password and even the email I used to register my original account lol. I tried explaining that to the support email peeps but they wouldn’t release the info. Which is probably a good thing round here. Thanks Shroomery...u rok my wrld
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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