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Clc420
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LC test to agar. Clean or bacteria?
#26973810 - 10/07/20 01:12 PM (3 years, 4 months ago) |
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Hey all, I've always had clean LC but this test plate I'm a bit stumped on. Not sure if it's just myc or is this bacteria? You can see myc growing out from the side but I wanna know what you think. Usually when I've seen bacteria it's usually a perceft circle.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26973851 - 10/07/20 01:35 PM (3 years, 4 months ago) |
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Usually pics of petris are pretty good but those pics are nearly worthless, can't make anything out of it. If it's a test plate you won't mind taking off the lid and taking a better picture, right?
LC test plates always grow very disorganized, even more so if you squirt more solution than needed on the plate, which I think it was your case, so even a clean test plate can look contamed to the untrained eye.
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26973876 - 10/07/20 01:46 PM (3 years, 4 months ago) |
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I have the flow hood running as we speak. I'll take a shot. Yeah the pictures are shot I have to admit. I struggle to get clear shots.
I did put too much on the plate yes and the LC has moved around as you can see.
Here's a shot of it with the lid off
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26973894 - 10/07/20 01:57 PM (3 years, 4 months ago) |
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That looks clean to me. Bacteria would have made itself apparent way before myc had a chance to recover. That's your typical clean test plate looking disorganized as usual and all over the place from having used too much LC. Just a drop or two is enough next time. IMO you are good to go or maybe give it a day or two if you want to be sure.
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26973914 - 10/07/20 02:07 PM (3 years, 4 months ago) |
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Thanks for your input mate.
I had some LC from a friend and bacteria showed up before the myc like you said it was a perfect circle. The myc grew straight over it in the end.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26974187 - 10/07/20 05:08 PM (3 years, 4 months ago) |
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I'm on my pc now and am having a better look at it. That myc looks very suspicious, almost as if it wasn't cube myc, really weird. Definitely give it a couple days to know what's up. How long since you put the LC on the plate?
Edited by Josex (10/07/20 05:15 PM)
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26974720 - 10/07/20 11:58 PM (3 years, 4 months ago) |
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It's been on there for 7 days.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26974728 - 10/08/20 12:10 AM (3 years, 4 months ago) |
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i shouldn't say this because LC plates with way too much myc on them are tricky to ID but i think it's clean. I'd highly advise getting a loop and doing further LC tests with an nice clean streak. It really makes testing LC easier.
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Josex
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 Yeah I used to use an inoc loop the few times I ever tested LC's. Fortunately, I don't need no testing no more because I never fuck up LC and the couple times I fucked up in the past I was able to tell by just looking at it.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Josex] 1
#26975362 - 10/08/20 11:48 AM (3 years, 4 months ago) |
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Quote:
because I never fuck up LC
Oooooh. I thought that once. Best of luck to ya but i went from thinking that back to testing them because catastrophic spawn loss is a bitch.
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Josex
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I think you can do some things that'll make it a lot harder on you to spot a bacterial LC. Are you still rocking 2% LME? Because it's orders of magnitude easier to tell a bacterial LC by doing just doing 0.2%. Also, I don't use magnetic stirrers these days because I don't mind the extra time and it also makes it easier to know what's up. I never had a clean looking LC show otherwise on grain, the day that happens I will change my mind.
Edited by Josex (10/08/20 12:41 PM)
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26975428 - 10/08/20 12:44 PM (3 years, 4 months ago) |
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Don't "chance" your mind. It's too valuable. HA. funny type. I do still use 2% and i do still like stirrers. I've done plenty of LC without a stirrer though. I've still been surprised. I'd say i generally can tell and all goes exactly as i planned when i streak the LC. But i have been surprised and the amount of time it takes me to verify an LC on agar is well worth it to me.
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Mateja


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Quote:
verum subsequentis said: the amount of time it takes me to verify an LC on agar is well worth it to me.
What's the point in testing a LC on agar if you're at the same time compromising the broth, exposing it to future potentian contamination in that very instant that you're testing it for current/present time cleanliness? Maybe I'm missing something here so please fill me in on what I've possibly misinterpreted here what's you're guarantee that a LC broth that has been hermetically tampered with on multiple occasions can somehow still be verified as clean?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975484 - 10/08/20 01:17 PM (3 years, 4 months ago) |
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That's a great point. It used to sketch me out really really badly. All i can say is that if you have 99.999% contam free sterile sessions then you should be able to test it without pooping in it.
Any one with much flow experience can verify that a nasty lurking in a culture is very very different than something airborne of something that is the result of insufficient utensil sterilization and use.
If you're aware of how to operate in the flow (or sab) then you should be able to sneak a piece of myc very easily without pooping in the LC.
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Mateja


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Quote:
verum subsequentis said: if you have 99.999% contam free sterile sessions...
That kind of success rate during sterile work is about as far away from realistic expectations as could be for the average cultivator, especially for beginners trying to learn the basics. Furthermore your proclaimed success rate regarding sterile work really begs the question as to why you even bother to test your LC's when you statistically might not even encounter one single contaminated broth during your whole lifetime, or how do you justify the extra precautionary work in testing the cleanliness of something that you consider 99.999% clean anyway?
-------------------- Cakes inside Water Tub
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975536 - 10/08/20 01:49 PM (3 years, 4 months ago) |
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Quote:
Any one with much flow experience can verify that a nasty lurking in a culture is very very different than something airborne of something that is the result of insufficient utensil sterilization and use.
I may not have been clear. I'm differentiating between an airborne or tool riding contam and a contam that is lurking in your culture. I did not claim to have a 99.999 percent success rate. i said if you..... to prove a point. That point is simply that it isn't hard to open and close something in the flow without shitting in it.
I have never and will never suggest that noobs should try to rely on LC.
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JHOVA
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975540 - 10/08/20 01:51 PM (3 years, 4 months ago) |
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Instead of testing with a loop you can "test" on a pf cake or grain jar one of cake aint the biggest loss in the world
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Mateja


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Quote:
verum subsequentis said: I have never and will never suggest that noobs should try to rely on LC.
Interesting, so what kind of requirements/experience do you feel is necessary for a grower to achieve first before it would be reasonable for him to for example start making cakes from LC's instead of LI's?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975632 - 10/08/20 02:46 PM (3 years, 4 months ago) |
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Jhova. That is very true.
Mateah. I personally think LI is harder to pull off than LC. I wouldn't say there is any bar that must be reached. I just advise noobs to get really reliable with agar to grain before fucking with liquids.
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Clc420
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I've always had clean LC but this plate concerned me. Yeah I've put too much on the plate, most definitely.
I work with a hood and I have a good sterile Technique. I always test my work, I'd rather take a extra step to make sure than regret it later on.
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