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Clc420
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LC test to agar. Clean or bacteria?
#26973810 - 10/07/20 01:12 PM (3 years, 3 months ago) |
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Hey all, I've always had clean LC but this test plate I'm a bit stumped on. Not sure if it's just myc or is this bacteria? You can see myc growing out from the side but I wanna know what you think. Usually when I've seen bacteria it's usually a perceft circle.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26973851 - 10/07/20 01:35 PM (3 years, 3 months ago) |
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Usually pics of petris are pretty good but those pics are nearly worthless, can't make anything out of it. If it's a test plate you won't mind taking off the lid and taking a better picture, right?
LC test plates always grow very disorganized, even more so if you squirt more solution than needed on the plate, which I think it was your case, so even a clean test plate can look contamed to the untrained eye.
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26973876 - 10/07/20 01:46 PM (3 years, 3 months ago) |
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I have the flow hood running as we speak. I'll take a shot. Yeah the pictures are shot I have to admit. I struggle to get clear shots.
I did put too much on the plate yes and the LC has moved around as you can see.
Here's a shot of it with the lid off
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26973894 - 10/07/20 01:57 PM (3 years, 3 months ago) |
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That looks clean to me. Bacteria would have made itself apparent way before myc had a chance to recover. That's your typical clean test plate looking disorganized as usual and all over the place from having used too much LC. Just a drop or two is enough next time. IMO you are good to go or maybe give it a day or two if you want to be sure.
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26973914 - 10/07/20 02:07 PM (3 years, 3 months ago) |
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Thanks for your input mate.
I had some LC from a friend and bacteria showed up before the myc like you said it was a perfect circle. The myc grew straight over it in the end.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26974187 - 10/07/20 05:08 PM (3 years, 3 months ago) |
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I'm on my pc now and am having a better look at it. That myc looks very suspicious, almost as if it wasn't cube myc, really weird. Definitely give it a couple days to know what's up. How long since you put the LC on the plate?
Edited by Josex (10/07/20 05:15 PM)
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26974720 - 10/07/20 11:58 PM (3 years, 3 months ago) |
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It's been on there for 7 days.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26974728 - 10/08/20 12:10 AM (3 years, 3 months ago) |
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i shouldn't say this because LC plates with way too much myc on them are tricky to ID but i think it's clean. I'd highly advise getting a loop and doing further LC tests with an nice clean streak. It really makes testing LC easier.
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Josex
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 Yeah I used to use an inoc loop the few times I ever tested LC's. Fortunately, I don't need no testing no more because I never fuck up LC and the couple times I fucked up in the past I was able to tell by just looking at it.
#notestgang
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Josex] 1
#26975362 - 10/08/20 11:48 AM (3 years, 3 months ago) |
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Quote:
because I never fuck up LC
Oooooh. I thought that once. Best of luck to ya but i went from thinking that back to testing them because catastrophic spawn loss is a bitch.
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Josex
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I think you can do some things that'll make it a lot harder on you to spot a bacterial LC. Are you still rocking 2% LME? Because it's orders of magnitude easier to tell a bacterial LC by doing just doing 0.2%. Also, I don't use magnetic stirrers these days because I don't mind the extra time and it also makes it easier to know what's up. I never had a clean looking LC show otherwise on grain, the day that happens I will change my mind.
Edited by Josex (10/08/20 12:41 PM)
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26975428 - 10/08/20 12:44 PM (3 years, 3 months ago) |
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Don't "chance" your mind. It's too valuable. HA. funny type. I do still use 2% and i do still like stirrers. I've done plenty of LC without a stirrer though. I've still been surprised. I'd say i generally can tell and all goes exactly as i planned when i streak the LC. But i have been surprised and the amount of time it takes me to verify an LC on agar is well worth it to me.
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Mateja


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Quote:
verum subsequentis said: the amount of time it takes me to verify an LC on agar is well worth it to me.
What's the point in testing a LC on agar if you're at the same time compromising the broth, exposing it to future potentian contamination in that very instant that you're testing it for current/present time cleanliness? Maybe I'm missing something here so please fill me in on what I've possibly misinterpreted here what's you're guarantee that a LC broth that has been hermetically tampered with on multiple occasions can somehow still be verified as clean?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975484 - 10/08/20 01:17 PM (3 years, 3 months ago) |
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That's a great point. It used to sketch me out really really badly. All i can say is that if you have 99.999% contam free sterile sessions then you should be able to test it without pooping in it.
Any one with much flow experience can verify that a nasty lurking in a culture is very very different than something airborne of something that is the result of insufficient utensil sterilization and use.
If you're aware of how to operate in the flow (or sab) then you should be able to sneak a piece of myc very easily without pooping in the LC.
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Mateja


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Quote:
verum subsequentis said: if you have 99.999% contam free sterile sessions...
That kind of success rate during sterile work is about as far away from realistic expectations as could be for the average cultivator, especially for beginners trying to learn the basics. Furthermore your proclaimed success rate regarding sterile work really begs the question as to why you even bother to test your LC's when you statistically might not even encounter one single contaminated broth during your whole lifetime, or how do you justify the extra precautionary work in testing the cleanliness of something that you consider 99.999% clean anyway?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975536 - 10/08/20 01:49 PM (3 years, 3 months ago) |
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Quote:
Any one with much flow experience can verify that a nasty lurking in a culture is very very different than something airborne of something that is the result of insufficient utensil sterilization and use.
I may not have been clear. I'm differentiating between an airborne or tool riding contam and a contam that is lurking in your culture. I did not claim to have a 99.999 percent success rate. i said if you..... to prove a point. That point is simply that it isn't hard to open and close something in the flow without shitting in it.
I have never and will never suggest that noobs should try to rely on LC.
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975540 - 10/08/20 01:51 PM (3 years, 3 months ago) |
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Instead of testing with a loop you can "test" on a pf cake or grain jar one of cake aint the biggest loss in the world
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Mateja


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Quote:
verum subsequentis said: I have never and will never suggest that noobs should try to rely on LC.
Interesting, so what kind of requirements/experience do you feel is necessary for a grower to achieve first before it would be reasonable for him to for example start making cakes from LC's instead of LI's?
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26975632 - 10/08/20 02:46 PM (3 years, 3 months ago) |
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Jhova. That is very true.
Mateah. I personally think LI is harder to pull off than LC. I wouldn't say there is any bar that must be reached. I just advise noobs to get really reliable with agar to grain before fucking with liquids.
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Clc420
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I've always had clean LC but this plate concerned me. Yeah I've put too much on the plate, most definitely.
I work with a hood and I have a good sterile Technique. I always test my work, I'd rather take a extra step to make sure than regret it later on.
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Forrester
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Quote:
verum subsequentis said: I personally think LI is harder to pull off than LC. I wouldn't say there is any bar that must be reached. I just advise noobs to get really reliable with agar to grain before fucking with liquids.
Agree with this, it's too often you see newer growers thinking they can just throw a mushroom tissue piece or some spore solution in LC broth and be good to go.
And even if they don't do that, many still think you can see contamination in LC and that if it's clear and not cloudy it's clean.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26976402 - 10/09/20 03:04 AM (3 years, 3 months ago) |
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Quote:
Forrester said: many still think you can see contamination in LC and that if it's clear and not cloudy it's clean.
Many = Every Microbiologist on earth including all of us that actually investigate and research the stuff that we have definitive opinions about.
The question now becomes, why did you apparently think that turbidity wasn't directly related microbial mass in a liquid media? If you have some document or research that support that notion then I'd be happy to look I to it 
https://sciencing.com/turbidity-indicate-microbiology-12703.html
Quoted this from the article in the link:
"While turbidity describes the general measure of suspended particles in a liquid, it is not reserved only for water, or for visible particles. Microbiologists use turbidity as a measure of cell density within a culture sample. Microbiologists use machines called photometers and spectrophotometers that shine different types of light through culture samples to determine turbidity. The general assumption is that the higher the turbidity, the higher number of cells within the culture."
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Edited by Mateja (10/09/20 06:37 AM)
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977274 - 10/09/20 04:25 PM (3 years, 3 months ago) |
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Oh dear God here we go again...
Nope. Not gonna do it. Go around spouting all the bullshit you want.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977276 - 10/09/20 04:25 PM (3 years, 3 months ago) |
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Again?
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Forrester
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Arguing with this guy. I have better things to do. If anyone else that doesn't put words in my mouth and ignore everything I say in response wants to disagree, I'm happy to oblige, but not with this dude again, it's not worth my time.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977353 - 10/09/20 05:18 PM (3 years, 3 months ago) |
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Quote:
Forrester said: Oh dear God here we go again...
Nope. Not gonna do it. Go around spouting all the bullshit you want.

And there you go again as well, personal opinions worded as scientific facts and then when I respond to the fallacy in your claim and back by response up with scientific documents and research and ask you about info or documents that your claim may originate from then you just start addressing everything non related but somehow always miss to adress key points in our conversations about science. That's weird considering growers look for your participation and value your willingness to answer posts and I'd assume there's no reason why you immediately feel the need to shut me completely down as soon as I challange one of your points or claims or whatever science related I'm glad you're not a moderator at least I guess this cancle culture is nonsense is contaminating the knowledge some of us are seeking, or in some cases just clarifications. I didn't come do throw tomatoes I have literally tons upon tons of opportunities to throw tomatoes on unfortunately designed claims, opinions, scientific formulations etc but I was mostly curious about how we can have arrived at seemingly exact opposite understanding of what turbidity and lack thereof means in context of colonizing LC broths. I'm still up for factual discussions/debates but I don't see you as my enemy nor anything other horrible that one can conjure in the moment of derailing into off topic.
I find talks about colonizing LC broths to be a fascinating as of lately, pardon my enthusiasm and fact checking insentives, I'm just being curious mayn
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977384 - 10/09/20 05:40 PM (3 years, 3 months ago) |
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All I said was you couldn't tell an LC was clean by the fact that it wasn't cloudy.
By your logic, my tap water should be sterile because it isn't cloudy.
That's all I need to say, end of discussion. Post all the links you want, it doesn't make the only statement I made, listed above and repeated for you, false.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26977468 - 10/09/20 06:45 PM (3 years, 3 months ago) |
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Quote:
Forrester said: All I said was you couldn't tell an LC was clean by the fact that it wasn't cloudy.
So this is one statement that I feel doesn't hold up from a scientific point of view. If you start with a freshly sterilized LC that has a very clear broth and after sufficient incubation time you still have the same clear broth then by all scientific research it should be concluded that your LC broth has no growth activity of any kind. This means that your sterilized LC still remains as sterile as it was fresh outta PC.
Quote:
By your logic, my tap water should be sterile because it isn't cloudy.
Now you're both misrepresenting my reasoning on the topic and you're misinterpreting the definitions of the science. First off it's not my logic that freshly poured tap water is sterile because it's not cloudy. But if your freshly poured tap water was incubated for a few days and still no turbidity was detectable then the science behind it will conclude that your tap water in that sample actually lacks growth activity entirely. But then again I can't argue using valid science on you're point about the tap water since tap water alone isn't considered a liquid growh medium thus can't be used as a tool to measure growth activity. Seems like you just need to refresh you're memory a bit on the subject of LC broth so you can express your views more clearly and be able to construct your arguments to be scientifically valid and thus can be discussed productively.
Now I feel we're getting somewhere with this, like we understand each other's posts a little better, I feel that we're learning and that's awsome
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Funky Monkey
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26977849 - 10/10/20 01:36 AM (3 years, 3 months ago) |
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Mateja


Registered: 07/14/16
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Quote:
Forrester said: Oh dear God here we go again...
I just read this thread again from start to finish and this time when I read everything I not only realized it's time for me to take a longer look at my self than I first thought  What worries me is I wasn't even aware of how much I misinterpreted everyone and the egoic nonsense Im doing is some of the cringiest most pathetic crap I've caught myself doing so far (I hope) hence what worries me, how many other times have I clearly crossed to line and it totally didn't register with me. JEEZ. I really hope this is the wake up call I needed to make a better change.
Quote:
verum subsequentis said: Again?
Hate to admit but its a pattern I really wish wasn't there, obviously I wish to be in a state where I can trust my own judgement in being able to recognize this ego maniac in my posting before Its too late, I can't even try to polish this turd I apologize to everyone in this thread and beyond, I don't even have the right to promise anything cause I can only hope that I will never again treat fellow cultivators this bad...
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Mateja] 1
#26978427 - 10/10/20 12:01 PM (3 years, 3 months ago) |
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Nah man, don't be lame. You do you it's alright. The only thing you need to change is the current pic you just put for your avatar and put back the war-fucking-mongering Jesus Christ cuz that's what's all about.
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JHOVA
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26978598 - 10/10/20 02:09 PM (3 years, 3 months ago) |
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If you want peace prepare for war
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Asura
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Re: LC test to agar. Clean or bacteria? [Re: JHOVA]
#26978651 - 10/10/20 02:52 PM (3 years, 3 months ago) |
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The wars always break out when LC comes up
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Asura]
#26979080 - 10/10/20 08:04 PM (3 years, 3 months ago) |
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I work with LC all the time and it almost never starts a fight.
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trippleblack
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i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
Edited by trippleblack (10/10/20 08:21 PM)
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979150 - 10/10/20 09:36 PM (3 years, 3 months ago) |
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i prefer a zig zag but it does the same thing.
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Asura
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Quote:
verum subsequentis said: I work with LC all the time and it almost never starts a fight.
LC is for lovers
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: Asura]
#26979161 - 10/10/20 09:48 PM (3 years, 3 months ago) |
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should be. If not it'll make a lover out of you if you stick with it.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979490 - 10/11/20 05:35 AM (3 years, 3 months ago) |
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Quote:
trippleblack said: i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
You guys saying it's impossible to know if the LC is good should try my LC tek for some months, start making 0.2% strength LME broths and maybe quit using stirrers for a while just for experimentation's sake. Then come here and tell me if you still can't spot the funk in an LC.
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trippleblack
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979571 - 10/11/20 07:45 AM (3 years, 3 months ago) |
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Quote:
Josex said:
Quote:
trippleblack said: i have run tons of lc lately
always always always test on a plate before dropping it onto anything bulk.. there is no way you can always tell if a lc is clean by just looking at it; even though it usually is if agar is good.
best way to test is apply a 1/4 inch dash in the center of the petri using an inoculation loop. maksure you to stir lc jar before testing. you will have your answer in 3 to 4 days. bacteria or mold slow the culture way down, you can tell very quickly if your culture is clean or not, that dash will be a perfect line of mycelium and the dash should spread out over the whole plate eventually.
You guys saying it's impossible to know if the LC is good should try my LC tek for some months, start making 0.2% strength LME broths and maybe quit using stirrers for a while just for experimentation's sake. Then come here and tell me if you still can't spot the funk in an LC.
why would anyone not test a liquid culture going to bulk? it only takes 3 or 4 days. not testing my bulk lc would be lazy for me not to do.
why would anyone not use magnetic stirrers? around a 2% solution of culture media is standard.. i run quart jars they are done within a week. I tested clear jars hiding mold before, rare but happens.
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979578 - 10/11/20 07:56 AM (3 years, 3 months ago) |
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I don't use stirrers because I don't need to, the extra time is not an issue if you have a good rotation going. I'm not saying people should not test their LC's, I'm not here to be politically correct or trying to make people do what I do, I'm here to share what I do, among other things. I could perfectly test them but I simply don't need to and you'd understand it if you did what I do.
For starters, 0.2% strength in an LC is already more nutrition than myc can use. Using 2% is pretty much worthless, this is not agar. That extra LME is there to cloud the broth, little else.
Edited by Josex (10/11/20 08:03 AM)
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trippleblack
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979593 - 10/11/20 08:08 AM (3 years, 3 months ago) |
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Quote:
Josex said: I don't use stirrers because I don't need to, the extra time is not an issue if you have a good rotation going. I'm not saying people should not test their LC's, I'm not here to be politically correct or trying to make people do what I do, I'm here to share what I do, among other things. I could perfectly test them but I simply don't need to and you'd understand it if you did what I do.
For starters, 0.2% strength in an LC is already more nutrition than myc can use. Using 2% is pretty much worthless, this is not agar. That extra LME is there to cloud the broth, little else.
i didn't mean 2% i meant .2% 2 teaspoons of powdered media per 500 ml is standard, thats within range by weight.
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verum subsequentis
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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26979897 - 10/11/20 11:56 AM (3 years, 3 months ago) |
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Josex, I prefer higher nute ratio because i've done tests and found they colonize faster and end up with more myc. Obviously, more than one (or two or three or four) tests must be done to find a semi definitive answer but that's what i have concluded so far.
I prefer to stir because i find they colonize much more quickly and end up being thousands of tiny myc fragments as opposed to a clump of myc. I like that better.
On your point of timing not mattering if you have a proper cycle going; I completely agree.
On the topic of being able to visually ID the cleanliness of low nute non stirred LC; I simply cannot speak because i don't have enough experience. I'd love to make a few up and see how it goes.
As far as whether or not it's necessary to test LC on agar; No, it's absolutely not. I prefer to because i've done it so much that it literally only takes a minute. I like to get my cycle rolling in such a way that it's just one more thing i throw into a sterile sesh. The time it takes to wipe down the jar, flame the loop and streak the plate is almost nothing.
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Josex
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Maybe myc can utilize better that extra nutrition if you stir the LC. 
I did sides by sides long ago doing LME broths that ranged from .2% to 3% strength and didn't notice much of a difference in growth if at all, but I wasn't using stirrers so... I posted the results in my LC thread iirc. Truth be told, the reason I don't use stirrers anymore is because I have a freaking steel tube in the jar that goes down to the bottom.  I prefer to use syringes over pouring these days so I'm fine with my freak ass jars and no stirrers. The tube helps break the myc just fine.
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verum subsequentis
seeker of truth



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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26979939 - 10/11/20 12:24 PM (3 years, 3 months ago) |
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interesting.
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Mateja


Registered: 07/14/16
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Re: LC test to agar. Clean or bacteria? [Re: Josex]
#26982032 - 10/12/20 05:55 PM (3 years, 3 months ago) |
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Quote:
verum subsequentis said: I prefer higher nute ratio because i've done tests and found they colonize faster and end up with more myc. Obviously, more than one (or two or three or four) tests must be done to find a semi definitive answer but that's what i have concluded so far.
Quote:
Josex said: I did sides by sides long ago doing LME broths that ranged from .2% to 3% strength and didn't notice much of a difference in growth if at all
This is kind of interesting that the three of us ended up with different conclusions testing the same hypothesis. When I did my testing I used 0.1%, 0.2%, 0.4%, 0.8% and 1.5% LME and in a way I came to similar conclusion as Josex because after a 25 day incubation all LC's seemed to have grown a similar amount of mass. Tho there was a significant difference in growth speed during the first 3weeks.
Below are comparisons between 0.1% 0.2% 0.8% and 1.5%
Day 12 of incubation
   
Day 18
   
Day 25 0.1%, 0.2%, 0.8%, 1.5%
   
Now Im curious about what kind of broths you've tested Verum and how significant were the differences in colonization in your experience?
-------------------- Cakes inside Water Tub
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verum subsequentis
seeker of truth



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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982050 - 10/12/20 06:02 PM (3 years, 3 months ago) |
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all my tests on done with stir plates. I think that makes a big difference.
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982056 - 10/12/20 06:06 PM (3 years, 3 months ago) |
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Mateah you do some great work in LC there, nice pics to match with the data. Love it.
I can't believe the difference in looks once you go over .5%! I may be PM'ing you with questions soon when I get back into growing, it's been a while for me but I love LC and don't keep shit for records
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26982076 - 10/12/20 06:19 PM (3 years, 3 months ago) |
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Thanks dude yeah visually comparable results definitely facilitate the insights about it more clearly
And also I made 15 LC's today with 0.05% LME, the broth is very clear and I just hope the colonies won't be negatively affected by this extremely low nutrient density. I don't wanna spoil any more but I'm hoping I have some nice results to post in a coming experiment thread soon
-------------------- Cakes inside Water Tub
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trippleblack
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26982267 - 10/12/20 07:55 PM (3 years, 3 months ago) |
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I use 2 grams per 1000 ml.. I love the pretty clear solution, my lc looks nothing like that, may try your recipe out. I use LME and a dash of soy peptone.
these are recent.. ready in 7 days. I usually test it on agar on about day 5.

the longer these lc sit, the more clear the solution get, looks like the mycelium eats up everything. not all cultures look the exact same in lc, these cordyceps like to spread around whole; the actives all collect at bottom(for these culutes anyway).
Edited by trippleblack (10/12/20 08:21 PM)
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: trippleblack]
#26982471 - 10/12/20 09:49 PM (3 years, 3 months ago) |
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Quote:
trippleblack said: I love the pretty clear solution, my lc looks nothing like that
You just reminded me that I should probably make another set of LC's but from rye water this time. It makes for a very clear broth no matter what but if diluted some then it's definitely clearer than even 0.1% LME I'd say. Well see soon.
-------------------- Cakes inside Water Tub
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Mateja]
#26986814 - 10/15/20 10:48 AM (3 years, 3 months ago) |
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Oh dear I've started something havent I 😂
Thanks for everyone putting in their input
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Josex
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26986843 - 10/15/20 11:08 AM (3 years, 3 months ago) |
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You did 
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Forrester
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Re: LC test to agar. Clean or bacteria? [Re: Clc420]
#26987213 - 10/15/20 02:39 PM (3 years, 3 months ago) |
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Quote:
Clc420 said: Oh dear I've started something havent I
It's almost guaranteed when you mention LC.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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Mateja


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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26987243 - 10/15/20 02:51 PM (3 years, 3 months ago) |
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-------------------- Cakes inside Water Tub
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Clc420
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Re: LC test to agar. Clean or bacteria? [Re: Forrester]
#26987269 - 10/15/20 03:08 PM (3 years, 3 months ago) |
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Quote:
Forrester said:
Quote:
Clc420 said: Oh dear I've started something havent I
It's almost guaranteed when you mention LC.
😂🙈 I wont bring it up in future haha
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