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Frrrunkis
yes

Registered: 06/14/20
Posts: 110
Last seen: 4 months, 4 days
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Planning ahead
#26973534 - 10/07/20 09:59 AM (3 years, 3 months ago) |
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Right now I've got some wrapped plates with clean myc in the fridge and 7 0,9L oat jars going. The jars are all a2g, each from a different plate. The plates are all from the same MS syringe.
I'm going to use the jars for shoeboxes, but I'm wondering about a sensible "next steps" plan using what I've got. The jars all have nice rhizo growth, they're going at different paces (some more advanced than others), but I'm pretty sure the speed of colonization is not enough for any decisions.
Should I use one of the jars for g2g, basically to keep some new jars going?
Should I use all of the jars for shoeboxes, see what comes up and clone from the fruits I like (and maybe make a spore print or two)?
Basically I'm trying to make a sensible roadmap. Any suggestions would be great.
-------------------- Literally nothing matters. This is fine.
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Hikeadellic
Fungi Fan



Registered: 08/31/20
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Loc: Appalachian Trail
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Not a bad idea to keep a jar back for the next generation. You can also go from grain back to agar if you really wanted too. As long as you stay sustainable by having many different projects going at the same time, you are on the right track. Once you have definite clone worthy fruits, you will upgrade your genetics by propagating them on agar.
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Frrrunkis
yes

Registered: 06/14/20
Posts: 110
Last seen: 4 months, 4 days
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Thanks, that's reassuring. 
Down to 6 jars after shaking today, oops:
https://www.shroomery.org/forums/showflat.php/Number/26973572
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redhandmat
Dude


Registered: 05/09/19
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Its definitely weird that you had that happen to only one of your jars. Could be that the one jar was letting in contam through a gap or something.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Agar isn't the bests choice when working with syringes. There are going to be lots of other fungi and bacteria floating around, which will be favored by the agar. If using syringes, it's best to shoot directly into grains or brf. Save agar plates for cloning or use with spore prints. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Frrrunkis
yes

Registered: 06/14/20
Posts: 110
Last seen: 4 months, 4 days
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Quote:
redhandmat said: Its definitely weird that you had that happen to only one of your jars. Could be that the one jar was letting in contam through a gap or something.
It could be external contaminants, since I didn't really do my homework on filters and just used single layer micropore tape, then learned that the pores aren't very micro at all. I've ordered SFDs to use from the next batch on, I hope the other jars will survive in the meantime.
Quote:
RogerRabbit said: Agar isn't the bests choice when working with syringes. There are going to be lots of other fungi and bacteria floating around, which will be favored by the agar. If using syringes, it's best to shoot directly into grains or brf. Save agar plates for cloning or use with spore prints. RR
I'm a bit surprised by this. I thought an unknown MS (I ordered mine online) into grain is super risky. Agar basically allowed me to isolate the good stuff from contaminants and get something I could put into grain with reasonable confidence. It was tricky and took a few attempts, but seems to have worked in the end. Have I been going about this wrong?
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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It was surprising for me to see RR say this as well. Prevailing sentiment from everything I've seen on the shroomery has contradicted this. I was even admonished back in the beginning by a gang of people (plenty of TCs included) for simply following the first Tek I found (frank's), that had me going from spore syringe to grain jars. I was told to put on my big boy pants and do it the "right way" using agar to clean things up before putting anything to your prescious prepped grains lol
So RR, you are saying that we should do a series of small grain jars to get lucky with at least one or two, grow those out and clone from there? You prefer and recommend that compared to cleaning up MS on agar first before going to quart grain jars, or in my case 6 pound bags?
Sorry if I am just repeating it with different words LOL I'm just trying to be super clear, because I've got a thick skull and it's having a hard time soaking in the contradiction of such a common instruction that is ground into every member that lays their fingers to a keyboard around here, especially when the contradiction comes from such a legendary/trusted source on the shroomery.
Edited by Funky Monkey (10/11/20 12:27 PM)
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Hikeadellic
Fungi Fan



Registered: 08/31/20
Posts: 1,227
Loc: Appalachian Trail
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Just like how there is many ways to skin a cat, there are many ways to shroom
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Yes...
Thank you Hike.
You are most certainly correct on that one. ππΌπ

Btw, belated welcome to the shroomery! π₯³
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RR? Am I on track with what you were trying to say for the scenarios I outlined with people's myco quarts, and grain bags, or am I mischaracterizing what you meant? Sorry for being so dense lol, but at my ego's peril I had to ask for some clarification for all us noobs as well as those of us that feel like they recently graduated from their noobiness only to feel like a noob-noob again reading what you wrote lol. Flying in the face of current encouraged/accepted practice is of COURSE okay, and doubly so coming from you, but I just want to make sure for sure that this is what you intended/meant before it starts getting quoted and endlessly bandied about by every member new or old that doesn't feel emboldened to add agar to the mix yet lol
Edited by Funky Monkey (10/11/20 12:26 PM)
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redhandmat
Dude


Registered: 05/09/19
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Quote:
Funky Monkey said: It was surprising for me to see RR say this as well. Prevailing sentiment from everything I've seen on the shroomery has contradicted this. I was even admonished back in the beginning by a gang of people (plenty of TCs included) for simply following the first Tek I found (frank's), that had me going from spore syringe to grain jars. I was told to put on my big boy pants and do it the "right way" using agar to clean things up before putting anything to your prescious prepped grains lol
Have to second this. Even if first plate is not clean and have other shit with it, its usually easy enough to arrive at a clean plate by transferring early on. Not that the grains themselves are precious or have a too high value (at least for me) but rather the fact that its a waste of time of just shooting a bunch of jars and having them contam on me. At least with agar, provided you save plates, you can go backwards and forward in the process. On top of that add the fact that we can use different kinds of antibiotic agar on the first couple plates: potential of successfully shrooming is greatly increased.
Of course other ways of doing it also work. Doesn't mean they work more often though. But Im still a noob of course and open to learn more.
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