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OfflineDonkeyHodie
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Registered: 03/30/18
Posts: 37
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Pan Bisprus syringes: MacMerdin's cakes to clone or direct swab to agar?
    #26972583 - 10/06/20 05:18 PM (3 years, 3 months ago)

Pan people, which do you think would be the best route? I have one syringe of Pan bisporus, and I'm trying to decide whether to drip it on a swab and swipe some plates, or just do some of MacMerdin's Pan-cakes and try to get a clone.

I know, I could do both. Agar first, then jars because the syringe will be at its cleanest before sticking them in doodoo, but I would have to do two loads in the pc then, because if I cook my agar with my jars, they'll be overcooked, and I'm lazy. Probably not too lazy to just go ahead and do both if I'm running two loads anyway, but I don't wanna.

Either way I still have to run a load for agar when I clone, so my options are 3 pc loads, and setting up the sab twice, or 2 pc loads and setting up the sab once. Of course, then I'll still need to set up the sab at least 1-2 more times for transfers.

Ugh, I'm gonna be burned out on this before I even get started. Maybe I should just do MacMerdin's > prints > syringes > MacMerdin's > repeat and skip the sab bullshit entirely. I really do hate all that fiddeldy agar stuff even though I do usually have pretty good luck with it. Even back when I was using peroxidated agar without the sab and just doing cube clones from PF jars, which I didn't hate. I guess it's just the sab I hate. Everybody says peroxide is shit now, though.


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