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Shop: Mushroom-Hut Substrate Bags   North Spore Cultivation Supplies, Injection Grain Bag

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InvisibleJennyJackston
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What is the ideal temperature range for colonisation of jars after inoculation?
    #26970102 - 10/05/20 03:38 AM (3 years, 4 months ago)

I inoculated some WBS and some Rye Grain jars with agar culture in my new SAB and was wondering what is the best temperature range to keep them during the colonisation process?

I have some heating mats which I keep under some blocks of granite and then I put some tote boxes on top. So the the temperature inside the box ranges between 24-28C.

The room that I keep these in is at around 21-22 Deg C.

I am also wondering what’s the ideal temperature to keep my agar dishes which I also inoculated.

I keep everything in the same room and there are various temperture points in the room. The floor being cooler than the rest of the little room.


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Offlineroarkell
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Registered: 08/29/19
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Re: What is the ideal temperature range for colonisation of jars after inoculation? [Re: JennyJackston]
    #26970120 - 10/05/20 04:09 AM (3 years, 4 months ago)

Anywhere from 65*F to 80*F... lower temps will slow colonization, while higher temps can accelerate it... but the same goes for contams... higher temps give contams a better chance.

(Sorry about the conversion, I'm a silly american...lol)


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InvisibleJennyJackston
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Re: What is the ideal temperature range for colonisation of jars after inoculation? [Re: roarkell]
    #26970772 - 10/05/20 02:59 PM (3 years, 4 months ago)

Thank You Roarkrell.  Thats about the range that I keep my jars.


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Offlineroarkell
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Re: What is the ideal temperature range for colonisation of jars after inoculation? [Re: JennyJackston]
    #26970840 - 10/05/20 03:58 PM (3 years, 4 months ago)

my room has been running a steady 70f lately and it has slowed some of my projects to a crawl... some genetics don't mind while other will have nothing to do with it... lol


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OfflineFlufferNutter
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Re: What is the ideal temperature range for colonisation of jars after inoculation? [Re: roarkell]
    #26970858 - 10/05/20 04:09 PM (3 years, 4 months ago)

I keep an incubation cabinet for plates and stuff at 76°-78°. It's been a good temp for them. Jars and tubs and such create their own heat after they get moving, so room temps, as has been stated, will always be good for any larger colonization. I like keeping a heated chamber for agar work because it keeps growth even and timely. The new forming colonies appreciate the effort.


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