Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMichael.o.logist
Stranger
Registered: 12/06/18
Posts: 1
Last seen: 5 years, 1 month
How long will sterilized grain last?
    #25718436 - 01/02/19 10:47 AM (5 years, 1 month ago)

I sterilized rye grain in my PC a couple days ago and won't be able to noc in my SAB for another 24-48 hrs. Will the moisture content be okay until then or should I make some fresh?


Extras: Filter Print Post Top
Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 6 months, 8 days
Re: How long will sterilized grain last? [Re: Michael.o.logist]
    #25718438 - 01/02/19 10:48 AM (5 years, 1 month ago)

Yeh, you'll be fine.


Extras: Filter Print Post Top
OfflineGeinstein
Shroomery addict
Male User Gallery


Registered: 01/25/18
Posts: 1,784
Last seen: 2 years, 1 month
Re: How long will sterilized grain last? [Re: teladi]
    #25718446 - 01/02/19 10:52 AM (5 years, 1 month ago)

You'll be fine dude


--------------------


Nothing breads nothing


Extras: Filter Print Post Top
Offlinebloozmahn
Dude
 User Gallery
Registered: 05/22/18
Posts: 147
Last seen: 1 year, 3 months
Re: How long will sterilized grain last? [Re: Geinstein]
    #25718585 - 01/02/19 12:01 PM (5 years, 1 month ago)

i've gone weeks.. it's why you sterilize in the first place.


Extras: Filter Print Post Top
OfflineGeinstein
Shroomery addict
Male User Gallery


Registered: 01/25/18
Posts: 1,784
Last seen: 2 years, 1 month
Re: How long will sterilized grain last? [Re: bloozmahn]
    #25720113 - 01/03/19 12:23 AM (5 years, 1 month ago)

Yeeeee if you know anything about the sterilizing of grains for mushrooms
You'd know to not push it too long

The grains aren't 100% sterilized even with 3 hours with 15 PSI


--------------------


Nothing breads nothing


Extras: Filter Print Post Top
InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: How long will sterilized grain last? [Re: Geinstein]
    #26969073 - 10/04/20 12:59 PM (3 years, 4 months ago)

I have a couple jars I didn’t have enough spores for. They have been sitting 1-2 weeks. I’m going to knock them up with the new spores I just got and see what happens. I believe they will be fine up to 2-3 months, technically If you think about it. As long as you “properly sterilize” your grains, if you were to inoculate them within a couple days and then the spores take 3-8weeks to become fully colonized. They are still sitting for so long. So whether you have spores inoculated or not. 2-4 weeks of sterilized grain sitting should be fine. I just wouldn’t go over 6months. Even on some venders pages that sell pre sterilized grain bags, they say this can sit on a shelf at room temp for weeks before use.


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 24 days, 19 hours
Re: How long will sterilized grain last? [Re: NecroMyce]
    #26969175 - 10/04/20 02:09 PM (3 years, 4 months ago)

This topic comes up a lot. To the OP, 24-48hrs is no big deal. You'll be fine.

To people who believe grain can be usable for months after sterilizing,that's not exactly true. We don't truly sterilize grain. The air pockets make proper sterilization tricky..

What we do is kill most potential contaminants, but not all of them.  The pc cycle opens a window of opportunity for our mycelium to get the upper hand, which is good enough for our purposes.

If you find that hard to believe (like I did when I first heard), give This thread a read through. Tons of good info there, and while there are some nay sayers in the beginning of the thread, none of them are able to prove otherwise, dispite a few attempts. And plenty of TCs offer great info.


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: How long will sterilized grain last? [Re: One of Us]
    #26969215 - 10/04/20 02:26 PM (3 years, 4 months ago)

Quote:

One of Us said:
This topic comes up a lot. To the OP, 24-48hrs is no big deal. You'll be fine.

To people who believe grain can be usable for months after sterilizing,that's not exactly true. We don't truly sterilize grain. The air pockets make proper sterilization tricky..

What we do is kill most potential contaminants, but not all of them.  The pc cycle opens a window of opportunity for our mycelium to get the upper hand, which is good enough for our purposes.

If you find that hard to believe (like I did when I first heard), give This thread a read through. Tons of good info there, and while there are some nay sayers in the beginning of the thread, none of them are able to prove otherwise, dispite a few attempts. And plenty of TCs offer great info.



So would you suggest “sterilized” grain that has sat for 1-2 weeks would be okay generally? And if possible could we re sterilize in the PC a second time?


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 24 days, 19 hours
Re: How long will sterilized grain last? [Re: NecroMyce]
    #26969259 - 10/04/20 02:41 PM (3 years, 4 months ago)

One week would probably ok, two weeks is definitely pushing it.

You can refrigerate "sterilized" jars and it will improve your odds of success, or like you mentioned you can definitely pc them again when you are ready to inoculate.  I believe many commercial edible growers use a process where they do 2 "sterilization" cycles before inoculation.

Something else to keep in mind is that jarred or bagged grain can dry out again with enough time


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: How long will sterilized grain last? [Re: One of Us]
    #26969299 - 10/04/20 03:10 PM (3 years, 4 months ago)

Quote:

One of Us said:
One week would probably ok, two weeks is definitely pushing it.

You can refrigerate "sterilized" jars and it will improve your odds of success, or like you mentioned you can definitely pc them again when you are ready to inoculate.  I believe many commercial edible growers use a process where they do 2 "sterilization" cycles before inoculation.

Something else to keep in mind is that jarred or bagged grain can dry out again with enough time


thank you. I guess I will try it out since my jars are EXACTLY 7 days from
When they were PC’d. And I do 15psi for 2 hrs. And sitting at 72-75 room temp.

I’m going to experiment because I only have like 10jars to inoculate and I ironically have half the jars with foil still on them and the other half with no foil. So maybe the foil holds moisture content in and keeps them from drying out, and the other jars may dried up a little. I just checked on them  and still are visible water droplets in them, just a little
Tho, nothing that runs or is crazy. Hopefully this hasn’t promoted endospores to
Germinate.

But after reading that post that was linked, they say, “pc only gives you a upper hand to get cube spores to germinate and once fully colonized the mycelium is strong enough to fend off other contaminants for the most part”

I think it’s really a gamble past a week, and it also depends on the conditions the jars were sitting in. From what I read, to inoculate 10 jars with what is maybe $5 worth of spores is worth it unless you have time to redo the PC process and wait another 1-3 days after they cool. (Which would be your best bet) but I’m lazy rn and I’d like to know if I can wait a week with my setup and conditions.(obviously everyone process and situation is different so there is so many varying outcomes.)


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 24 days, 19 hours
Re: How long will sterilized grain last? [Re: NecroMyce]
    #26969329 - 10/04/20 03:34 PM (3 years, 4 months ago)

That sounds like a good experiment. Like mentioned in that thread, we definitely know we only have a limited window of opportunity, but how long this window is open is not as known. Most people don't like to push their luck, so it is as Hunter S Thompson said:

"The Edge... There is no honest way to explain it because the only people who really know where it is are the ones who have gone over."

But this open window time can change with many different variables, including individuals' setups and conditions, as you mentioned, but more importantly, I think this time frame will change from bag to bag of grains.  One bag of unprocessed, dry grains may have significantly more endospores than another, even within the same brand. So anything you learn from experimentation, may not translate to your next bag of grain. Now, I do not know this with certainty, but its worth mentioning.

That said, I think that going spore to grain is the real gamble you are taking


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How long will sterilized grain last? [Re: One of Us]
    #26969338 - 10/04/20 03:43 PM (3 years, 4 months ago)

2-4 days is fine. After about 2 weeks they will start dropping off like flies. You can sterilize and refrigerate to extend shelf life. I wouldnt push it past a month however.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: How long will sterilized grain last? [Re: maxmush]
    #26969354 - 10/04/20 03:53 PM (3 years, 4 months ago)



Probably been a month at least on these. I'll get to them eventually.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


Extras: Filter Print Post Top
OfflineAtmozFear
just a shade of myself
Male

Registered: 01/25/19
Posts: 1,032
Last seen: 1 day, 17 hours
Re: How long will sterilized grain last? [Re: One of Us]
    #26969391 - 10/04/20 04:13 PM (3 years, 4 months ago)

Quote:

One of Us said:
Most people don't like to push their luck, so it is as Hunter S Thompson said:

"The Edge... There is no honest way to explain it because the only people who really know where it is are the ones who have gone over."



That's a great quote!  eerie.


--------------------


Edited by AtmozFear (10/04/20 04:14 PM)


Extras: Filter Print Post Top
InvisibleNecroMyce
Mostly Ghostly
I'm a teapot


Registered: 05/12/13
Posts: 747
Re: How long will sterilized grain last? [Re: One of Us]
    #26969442 - 10/04/20 04:43 PM (3 years, 4 months ago)

Quote:

One of Us said:
That sounds like a good experiment. Like mentioned in that thread, we definitely know we only have a limited window of opportunity, but how long this window is open is not as known. Most people don't like to push their luck, so it is as Hunter S Thompson said:

"The Edge... There is no honest way to explain it because the only people who really know where it is are the ones who have gone over."

But this open window time can change with many different variables, including individuals' setups and conditions, as you mentioned, but more importantly, I think this time frame will change from bag to bag of grains.  One bag of unprocessed, dry grains may have significantly more endospores than another, even within the same brand. So anything you learn from experimentation, may not translate to your next bag of grain. Now, I do not know this with certainty, but its worth mentioning.

That said, I think that going spore to grain is the real gamble you are taking





Understood man! Thanks everyone that chimed in, def helped me and OP. Didn’t mean to hyjack this post but it was obviously very informative and productive.


BOTTOM LINE: your best rule of thumb is anything inoculated ASAP within a day or two
After PCing is the best way to do it! But if you have to
Wait a week you’ll probably be fine if your bags/jars are in proper sterile conditions and your filter patch or vent hole is covered to reduce losing moisture contenting inside your grains. And if you wait any longer than a week to inoculate, your odds are going lower and lower each day you wait, but if you decide to inoculate after 1 week, it’s possible but it’s a larger gamble. None the less, If you wait a month or anything longer than 1-2 weeks, you can also Re-Sterilize as well and you’ll be fine to inoculate once the bags/jars cool.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* Pre sterilized grain? Chemical_Smile 781 5 04/07/03 12:19 AM
by Chemical_Smile
* WET GRAINS! Anonymous 4,802 9 10/21/03 05:52 AM
by shirley knott
* Grain Tek Sterilization Zwieback0 2,676 7 10/17/02 04:48 AM
by Snobrdr311
* popcorn tek- healthy kit grain to 'corn innoc, need help! groovydan 3,379 6 03/27/03 06:25 AM
by groovydan
* Question about bacteria contam in grains robinhood2 1,600 8 04/16/03 07:57 PM
by Anonymous
* Electric Roaster Oven For Sterilization EnCHaNTeDPiXiE 2,275 15 08/24/03 03:57 PM
by Senovio
* Lightly simmering you GRAIN instead of soaking overnight.... YellowSubmarine 2,295 19 09/19/03 07:28 AM
by tak
* question about grain 2 grain innoculation robinhood2 2,375 12 09/22/02 01:17 PM
by matts

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,034 topic views. 22 members, 106 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.008 seconds on 14 queries.