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OfflineTreycherry
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Every ms inoculation ends up like this
    #26968605 - 10/04/20 06:10 AM (3 years, 4 months ago)

This will be my third attempt at putting the b+ spores to agar.
I’ve done a lot of golden teacher and Fiji agar work with 0 contam but anytime I try to start the b+ grow it ends up contaminated.

I use 1 vendor for all my syringes and consistently see this contaminated growth for my b+.

Is it actual contam or does the growth just have a different color ?



( the center appears slightly blue with white growth coming off of it )


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OfflineOne of Us
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Re: Every ms inoculation ends up like this [Re: Treycherry]
    #26968612 - 10/04/20 06:30 AM (3 years, 4 months ago)

Thats mold

I don't think you'll get any clean mycelium from that syringe


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OfflineTreycherry
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Re: Every ms inoculation ends up like this [Re: One of Us]
    #26968618 - 10/04/20 06:37 AM (3 years, 4 months ago)

I’ve tried 3 syringes now??

The other strains worked out fine but anytime I buy a b+ syringe this happens. I’m not sure what to do. I really want to try out b+ but at this point it’s impossible


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OfflineCaptainParanoid
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Re: Every ms inoculation ends up like this [Re: Treycherry] * 1
    #26968622 - 10/04/20 06:45 AM (3 years, 4 months ago)

Try a different vendor. I had no problems with B+ from a different vendor than you. They are a well known sponsor on this site.


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Offlinejcm4620
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Re: Every ms inoculation ends up like this [Re: CaptainParanoid]
    #26968661 - 10/04/20 07:30 AM (3 years, 4 months ago)

AGAR  learn it  know it  live it😃

injecting spores from a shringe is risky as it is. all spores are dirty to some extent and by cleaning it up on agar b4 inoculation will greatly increase your odds of a good grow.


🤦🏻‍♂️🤦🏻‍♂️🤦🏻‍♂️ i just saw that u are using agar lol ignore previous statements then lol i didnt c that. im on mobile and i totally overlooked that so my bad there but im glad ur using agar. id say then ur syringe is likely fucked amd to be honest tho man prints are so so so much better to use when knocking up agar plates.


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Edited by jcm4620 (10/04/20 07:37 AM)


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OfflineTreycherry
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Re: Every ms inoculation ends up like this [Re: jcm4620]
    #26969006 - 10/04/20 12:04 PM (3 years, 4 months ago)

I’m using agar to start my grow and every one of the growths appears to be a contaminant.

It’s just weird to me that any other strain from this vendor is clean, but anytime I order the b+ it’s bad


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InvisibleJHOVA
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Re: Every ms inoculation ends up like this [Re: Treycherry]
    #26969029 - 10/04/20 12:20 PM (3 years, 4 months ago)

Use a sterilized cotton swab with a single drop from your syringe and then make a Z streak. Do that on 3 plates. The second and third plate will most likely be much cleaner than the first. When you see germination take the cleanest mycelium at the tail end of the z streak.



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Offlineverum subsequentis
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Re: Every ms inoculation ends up like this [Re: JHOVA]
    #26969087 - 10/04/20 01:13 PM (3 years, 4 months ago)

:whathesaid:

Your syringe is dirty. If you really want to try and get something out of that syringe, do something like JHOVA suggested. Other wise hit up a different get a new B+ syringe or hit up the market place for a print.


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