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Josex
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Dem Dicks! GROWLOG. 3
#26968160 - 10/03/20 08:03 PM (3 years, 3 months ago) |
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A GROW FROM SWABS Never made a growlog before but suddenly got the itch so here it is. Very recently I finished some MS PE grows from a PE syringe (SW) that was gifted to me by an awesome member. They had a special look to them so I felt I had to take some swabs. Some pics I posted in POTD:  I took some nice dark swabs from these fruits:   So without further ado, let's get the show on the road! 4 October. I used 2 swabs to inoculate those 5 PF-tek pucks in the pic. You can learn more about them here. To inoculate the pucks I did the following procedure (from the tek above) that has worked really well for me in the past: Quote:
Josex said:
- Use a little filtered container to sterilize enough water to cover the bottom half an inch.
- In the SAB, take the swab and dunk the tip in the sterile water a few secs to soften up the cotton fibers and to rinse it off a bit.
- Take your scalpel and use the tip of the blade to remove a tiny, spore-laden piece of cotton from the swab.
- Bury the piece of cotton in the PF-tek puck.
Now the waiting game, will be updating this regurlarly. 
7 October.

Woke up today to some germination on 3 plates out of 5, nice. It's difficult to see in the pic but we have some dick myc right in the middle.
12 October.

It's really hard to take a good pic through the glass. All the pucks have some beautiful rhizo growth, pics don't make it justice.
Growth looks healthy enough for me so I decided to do something with them pucks. I took needle biopsies from 4 pucks and inoculated 4 little LC jars.

I know right?
If my regular LC jars are somewhat ridiculous, those above are just too much, I know. Made them 2 years ago as "LC starter" jars (to inoculate LC with LC), but this is actually the first time I'm using them.
I know you perverts want to see some cock asap so that's why I decided to use these. In each jar there's only 60ml of broth (.25% LME) and also threw a stir bar in each to speed things up, so I'm gonna be taking the dust off my magnetic stirrer for this one as soon as I see the first tiny puffs of myc in the liquid.
In case someone is wondering how the fuck I inoculated the jars, I just stuck the needle through that sophisticated self healing injection port called polyfil.

15 October.

We've got life in all the 4 little LC's. I started to stir them today, just a few minutes each.
18 October.

20 October.

These are 3 of the germination plates. All were inoculated with tiny pieces of the swab except the one in the first pic that got a big piece (you can actually see a piece of cotton sticking out if you enlarge the pic). Made the first transfers today and also made a couple LC's.
On another note, I'm a little disappointed on the 4 little 60ml LC's I started. They look good but they're taking more time than I was expecting.
23 October.

Out of the four 60ml LC's this is the only one I stirred when I felt like it and it looked done today. First and last time I'm using these little jars, fuck em. I thought they were going to be done far sooner.

I aspirated 50ml of LC and inoculated 8 jars of wheat like the one below, which has all sorts of shit mixed in that I didn't put there. 


One of the couple LC's I inoculated 3 days ago. It has .1% agar, hence the turbidity. I think I'm going to be adding agar to my LC's from now on, I'm liking it so far.
27 October.
 
These are the eight jars inoculated with the small LC four days ago. Time for a shake!

This is the 250ml LC (.2% LME and .1% agar) that was inoculated 7 days ago, but it was ready 2 days ago. The combination of stirring it and adding a bit of agar has shown to have a huge impact on the speed of colonization of the broth and it has also produced a substantial mycelial mass.

This is the LC resting. It won't settle because it can't settle. All the broth is colonized completely, top to bottom. I inoculated 8 jars of wheat. There's a lot of leftover LC yet.

This one was inoculated the same day (200ml LC, .2% LME and .1% agar). This one didn't get stirred but was swirled a couple times a day instead. Not bad. I inoculated another 8 jars with this one.
8 November.

These are the 8 jars inoculated with the mini LC after a recovery shake at 100%. Made a tub today, 4 qts of spawn to CVG (1:2). The reason I didn't spawn the other 4 jars is because I don't trust the wheat I got and I'm working with some millet now that I have a good feeling about, so I inoculated some jars of millet with an LC from one of the cultures from swabs that looked particularly good.

They were inoculated on November 5 and this pic was taken 72 hours after inoculation. Haven't had that recovery in a while. Myc seems to be loving the millet and if myc loves it I love it too. Gave 'em a shake today.
17 November.
 
I let the millet jars consolidate a bit and spawned 3 of them to CVG (1:3).
I'm interested in seeing how the millet will do. I just wished I had used it from the beginning of the growlog, because the wheat I'm using is definitely sketch and I'm having problems with it with other grows. Well at least it's going to make for an interesting side by side, millet vs wack wheat.
Since my RH is pretty high I figured I could do with 3 extra holes at the top of each short side for the ez dial.
28 November.

Wheat tub with the casing mostly colonized. Me thinks we'll see pins in a matter of a few days.

The round wheat bucket getting there.
I have a bunch of tubs coming from different cultures that I got started from those 2 swabs I mentioned at the start of the growlog. Cultures look really good on the PF-tek pucks and so aggressive they start to blob up right upon hitting 100% colonization, which I take it to be a good sign.
5 December.
 
This is a first for me shit's almost embarrassing, shrooms growing on the sides and nothing on the surface yet. This culture definitely didn't like that super thick casing. Too many things I tried for the first time here and too many things that went wrong as well.
But the game continues, have some more stuff from those swabs coming along, all cased by now;
  
17 December.
 
I've got several tubs that are blobbing right now, no pins. Fortunately I have 2 millet tubs from a different swab culture that are starting to pin.

This is the side pinning bastard today. Shitty grain, shitty prep and shitty spawn, there's little more to it. I'm not tossing this culture just yet because it's putting out some cool looking monsters and want to see what it can do with better spawn.
Same culture, same spawn, being weird and blobbing a bit in a bucket:

20 December.
 
Two different tubs of culture #5 from swab. These might turn out nice I think.

Culture #1 blobbing all over the place.

Culture #3, which is the same one from the tub I posted earlier which put out some 3 digit stuff. Pics after harvest yesterday;
 
22 December.
 
Dem peens today. At least we know culture #5 delivers. Going to clone something from that clusterfuck when the tubs are ready.

Culture #3 (the same culture as the tub posted earlier that was side pinning). Seems to keep pulling out some phatties. I want to see if the bucket can produce some 3 digit stuff too.
24 December.

In these LC's are the last of the cultures that were still waiting their turn to be fruited out. Culture #6 looks amazing too but I just didn't have a free LC jar for it, so that one it's going to be last to be fruited.
From each jar I inoculated 5 jars of barley spawn today.
If anyone is wondering what the hell is "cloneT", here's the story:
Quote:
Josex said:
Quote:
Josex said:
On another note,
Quote:
Josex said:

Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...

I dipped my inoc loop into both jars and streaked a couple agar plates. In one of them I got mold but the other jar tested clean.

I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.

Found this little round phat guy today in the test plate. Guess who's gonna be cloned today.

26 December.
Culture #5, tub 1:
 
The chunkiest of the lot:

Final weight from tub 1, can't complain from MS and 3 quarts of millet:

Culture #5, tub 2 which I could've let it ride a bit longer (was spawned 2 days after tub 1), but needed to harvest today:
 
The chunkiest of the lot:
 
Final weight from tub 2:

Culture #3, bucket:

Sum crazy shit:
    
Culture #1, decided it was time to pin after all those blobs:

4 January.
Time to harvest culture #1 from the bucket:

Some moar freaks, it's not even funny anymore:

Mutant family


I'm not even sure if these are safe to eat 
Current tale of the tape:
- Culture #5: keeper.
- Culture #3: worth giving another try imo.
- Culture #1: would like to keep it for weird but it's terribly slow and yields poorly.
Coming next cultures: #2, #4 and #7. Culture #6 will be last to be grown.
28 January.
Culture #7 (spawned on 1/13), colonizing the casing aggressively:

Culture #4 (spawned on 1/10), this one likes it slower:

Culture #2 (spawned on 1/13):

Also, just inoculated a bunch of jars with culture #6 to finish the growlog.
Edited by Josex (01/28/21 10:25 AM)
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex] 2
#26968161 - 10/03/20 08:03 PM (3 years, 3 months ago) |
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THE LONE SURVIVORThis is a second growlog that is closely related to the one above. On July 7th I inoculated 5 PF-tek pucks with the same PE syringe I mentioned in the OP. The spores germinated fast and I made 2 transfers from every germ plate to new pucks. I have to admit I like to take clones from these pucks because they usually pin heavily. Even APE pins very well on these without a casing, some pics from a couple years ago:  The problem is that these PE pucks didn't want to pin no matter what. I wasn't too surprised by this, though. Too bad because I was hoping to snatch some easy clones. 40 days in and the pucks didn't even have some sad knots to show so I took the bait, dunked the pucks a few minutes and then applied a crappy casing layer by sprinkling some verm straight out of bag on all pucks and then misted the verm down until dark. NOTHING... 3 weeks went by after casing and still the pucks didn't want to pin at all. I didn't want to toss them just yet so I just forgot about them. Lo and behold, on September 12th I was preparing to toss them all when I found this in a puck:  Big phat pin. The only one out of 15 pucks. The Lone Survivor. 4 October.So of course I cloned that bastard. Two transfers in and looking pretty good so I made an LC today. Nothing to show but some liquid in a weird jar. 12 October. That's the 300ml LC eight days in. I could use it right now but I'd rather let it go a bit longer so there's more myc. I think it's gonna be ready in 5-6 days. 16 October.  On the left, the 300ml LC I've been posting since the beginning and on the right, a 250ml LC (.2% LME and .1% agar) that I inoculated with the same clone 2 days after I inoculated the first LC. I wasn't planning on making a second LC so I didn't even posted it. I don't know what I was thinking putting all the eggs in one basket for the growlog so I made the second LC as a backup in case something went wrong with the first one. And thank god I did because shit did indeed happen. I was trying to aspirate the LC when I realized the tube was completely stuck. It almost never happens but it does happen sometimes and it had to happen for the growlog, right?   Making the backup LC was not in the original plan but now that I had it I was planning on doing a side by side (regular LC versus agar LC) but now it's only going to be the agar LC, bummer.  Have to say it's the first time I'm adding agar to an LC (was inspired by Nate's TEK, although I used half the agar he says in the tek) and was greatly surprised to see how thoroughly myc colonizes the broth with the help of a bit of agar. There were not uncolonized parts of liquid to be seen like it always happens with regular LC's and myc seems to colonize the broth faster too. The only drawback is that the agar clouds the liquid big time and I think it would be downright impossible to notice the usual visual signs of contamination in the LC. Made some nice syringes and inoculated a bunch of jars of wheat.  I ordered the wheat online because the grain I can get locally is horrible stuff. But take a look at this online shit...  Doesn't look good at all and it's so difficult to hydrate. This is the best I could do.   I'm a little bummed with the grain situation but if this wheat doesn't work I will try another. 27 October.  This wheat is giving me shit as I expected. These are eleven days in and they're not done yet. Shook them at inoculation (I never do that, just wanted to check something out) and then shook again when the LC recovered, and they have received today what I hope will be the last shake. I don't trust this wheat at all so I'm going to be making another LC with this clone and will use the same wheat I'm using for the first growlog. Regardless, I hope it works out because I inoculated all those jars you can see in the pic. Don't know what I was thinking going so big with a suspect grain. 3 November.  These are some jars after a last recovery shake. I'm not happy how they're looking. Spawned only 4 jars to CVG (1:2) and made my first ez dialed tub ala Pasty.  Depending on how the spawn behaves in the next few days I'll decide whether or not to spawn the rest of the jars. 8 November. This is the tub 5 days in. It isn't looking bad but it could look better and it gives off a slightly bacterial smell. I'm going to case it soon. In the meantime, I'm still holding the rest of the jars in the pic. I'm still not liking the spawn and I'm not very happy how it's performing in the tub, so I may toss these eventually and make some millet spawn instead. 11 November. Cased it,  Since I can't seem to find plain peat without any additives, I've been using some potting soil for cactus for all my PE grows. For all of you having the same problem, know that you can use this. I always get such a nice coverage of the casing and very good surface conditions, myc loves it. The prep is the same as the usual 50/50 peat/verm casing, with lime and gypsum too. This is the stuff,   Also made a bucket with this clone today, 3qts to CVG (1:2). And another bucket for the first growlog which I won't bump for such a small update.  Also noc'd some millet jars today with the LS clone. 28 November. I know it's nearly impossible to see them in the pic but we have the first pins starting to pop up. 5 December. The pinset ain't precisely breathtaking but neither was the spawn, nonetheless I have the impression these fruits are going to be cool when grown out. I still have faith in this clone. Here's a tub I cased recently, same clone; 8 December.  They have a long way to go yet. Yield's gonna be shitty but man aren't they purdy. For reference, this is a 60L tub. 11 December.  First tub of the LS clon done. Some 3 digit monsters: 17 December. Here's the LS clone on millet spawn. I think we're going to see pins very soon. 11 January.So the tub looked and smelled fine before casing it and after casing it too (at first) but something weird happened:   The sparse few fruits took too long to pop up and growth is obviously stunted. There's also some fuzz on the caps that looks like some kind of mold, just kept it this long out of curiosity to see what'd do. These last days the tub was giving off an earthy smell so I was expecting trich at any moment. No trich but some kind of mold started to pop up on the surface (circled in red in the pic), so I had to toss the tub. Broke the sub apart to inspect and it looked and smelled fine. The earthy smell seemed to come from the casing only. Same issue with the same clone, this time in a bucket:  I'm suspecting the casing. I got lazy and used some jars with casing material that had been sitting for way over a month on a shelf after pasteurization. Had the exact same issue with a tub of KSSS that also got cased with the same old casing material. These tubs were looking great from the beginning so I was stoked to see how they'd perform from what I thought was better spawn than the spawn used for the previous grow. Guess I'll have to start over.
Edited by Josex (01/13/21 06:04 AM)
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mushboy
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26968167 - 10/03/20 08:08 PM (3 years, 3 months ago) |
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A double grow log?
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Josex
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
#26968175 - 10/03/20 08:12 PM (3 years, 3 months ago) |
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mushboy
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Re: Dem Chodes! GROWLOG. [Re: Josex] 2
#26968183 - 10/03/20 08:16 PM (3 years, 3 months ago) |
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Burying swab fibers in the pf puck is a sweet idea too.
Nicely done stuff mang
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Tormato  
The Goddess Kali Meh 😛




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Re: Dem Chodes! GROWLOG. [Re: mushboy]
#26968194 - 10/03/20 08:26 PM (3 years, 3 months ago) |
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Nice!! 
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Pastywhyte
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Re: Dem Chodes! GROWLOG. [Re: Tormato]
#26968222 - 10/03/20 08:54 PM (3 years, 3 months ago) |
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Psicomb



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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26968223 - 10/03/20 08:55 PM (3 years, 3 months ago) |
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Great stuff Josex, I always love your threads. Always some weird shit goin on (and I mean that in the best way possible)
--------------------
When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Psicomb]
#26968277 - 10/03/20 09:34 PM (3 years, 3 months ago) |
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Thanks guys, let's see how this shit turns out! I'm all stoked like a fucking noob over their first cake.
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Camera93
We got dicks like Jesus



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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26968285 - 10/03/20 09:40 PM (3 years, 3 months ago) |
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-------------------- All I need are some tasty waves, a cool buzz, and I’m fine. Whatever you decide won’t really impact our survival Close your eyes, and do the best that you can
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JHOVA
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Re: Dem Chodes! GROWLOG. [Re: Camera93]
#26968339 - 10/03/20 10:21 PM (3 years, 3 months ago) |
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-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Tankie_J
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Re: Dem Chodes! GROWLOG. [Re: JHOVA]
#26969977 - 10/04/20 11:53 PM (3 years, 3 months ago) |
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Tankie_J]
#26973137 - 10/07/20 02:34 AM (3 years, 3 months ago) |
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Woke up today to some germination on 3 plates out of 5, nice. It's difficult to see in the pic but we have some dick myc right in the middle.
Edited by Josex (10/07/20 08:42 AM)
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alaskappalachian
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26973156 - 10/07/20 03:12 AM (3 years, 3 months ago) |
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-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Josex
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Not really an update. This is the puck where I put that fat PE pin I posted in the second growlog. Just now I found this little pin growing right off its resting father. I don't know if I should bother to clone it since it's already a clone. The thing is it's only taken 27 days to pin and that's faster than lots of non PE varieties I've had on pucks.
On another note, temps have gone down quite a bit here and it's really slowing things down. All 5 pucks I swabbed germinated in 3 days and they have what appears to be nice clean growth but just too little to work with yet. They're taking their sweet time.
Edited by Josex (10/10/20 11:09 AM)
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eatyualive
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26978405 - 10/10/20 11:40 AM (3 years, 3 months ago) |
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Nice josex! Did you ever see that burma chose i had?
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Josex
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Re: Dem Chodes! GROWLOG. [Re: eatyualive]
#26978412 - 10/10/20 11:44 AM (3 years, 3 months ago) |
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No Eats, show me the porn don't be shy.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26979494 - 10/11/20 05:49 AM (3 years, 3 months ago) |
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how do they compare potency wise to reggie cubes?
-------------------- The best things in life can be smelled on one's fingers.
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Josex
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I ate 2 grams and they were strong. The people I gave them away to aren't complaining either. I've had APE and KSSS that were stronger though. Just a matter of finding a nice potent clone I think.
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LemonTekno
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26979514 - 10/11/20 06:23 AM (3 years, 3 months ago) |
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Josex
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Re: Dem Chodes! GROWLOG. [Re: LemonTekno]
#26981834 - 10/12/20 04:06 PM (3 years, 3 months ago) |
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Some updates. Brace yerselves for the WEIRDNESS...
From the first growlog:
Quote:
Josex said:
12 October.

It's really hard to take a good pic through the glass. All the pucks have some beautiful rhizo growth, pics don't make it justice.
Growth looks healthy enough for me so I decided to do something with them pucks. I took needle biopsies from 4 pucks and inoculated 4 little LC jars.

I know right?
If my regular LC jar are somewhat ridiculous, those above are just too much, I know. Made them 2 years ago as "LC starter" jars (to inoculate LC with LC), but this is actually the first time I'm using them.
I know you perverts want to see some cock asap so that's why I decided to use these. In each jar there's only 60ml of broth (.25% LME) and also threw a stir bar in each to speed things up, so I'm gonna be taking the dust off my magnetic stirrer for this one as soon as I see the first tiny puffs of myc in the liquid.
In case someone is wondering how the fuck I inoculated the jars, I just stuck the needle through that sophisticated self healing injection port called polyfil.

From the second growlog:
Quote:
Josex said:
12 October.

That's the 300ml LC eight days in. I could use it right now but I'd rather let it go a bit longer so there's more myc. I think it's gonna be ready in 5-6 days.
Edited by Josex (10/12/20 04:19 PM)
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Becky G. Spot
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Re: Dem Chodes! GROWLOG. [Re: Josex] 2
#26981845 - 10/12/20 04:13 PM (3 years, 3 months ago) |
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I saw in another thread you were telling some guys that you were going to post some "new and improved" LC lid build design. Did that ever happen? I would love to see it if so.
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
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Josex
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No, I'm onto it though. Currently waiting for the parts that I ordered more than a month ago. This covid BS is really slowing things down.
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tryptkaloids
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982081 - 10/12/20 06:21 PM (3 years, 3 months ago) |
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Chillin here just because of your little jab
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Mateja


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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982119 - 10/12/20 06:51 PM (3 years, 3 months ago) |
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Quote:
Josex said:

That's the 300ml LC eight days in.
Homogenous in growth appearance tells me that there's only one type of filamentous organism in there. The lack of turbidity implies that there's no microbial activity present in the uncolonized parts of the broth. I like where this is going
-------------------- Cakes inside Water Tub
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tripdawg420
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982159 - 10/12/20 07:16 PM (3 years, 3 months ago) |
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982242 - 10/12/20 07:47 PM (3 years, 3 months ago) |
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Quote:
Mateah said:
Quote:
Josex said:

That's the 300ml LC eight days in.
Homogenous in growth appearance tells me that there's only one type of filamentous organism in there. The lack of turbidity implies that there's no microbial activity present in the uncolonized parts of the broth. I like where this is going 
Yeah I think it looks good too. My dumbass didn't hear the timer and cooked that LC ten minutes more than usual, that's why it's so dark, LME is delicate like that. It was a .3% LME broth, a tad more than usual, but it should have come out way clearer.
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Becky G. Spot
Good Necky Becky

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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982253 - 10/12/20 07:51 PM (3 years, 3 months ago) |
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I did a 0.2 concentration of LME and got TONS of sediment.
Why?
I am worried it's gonna clog the needle. Will the myc devour it? Or does it just attach itself to the outside of said sediment, and I am going to have to contend with chunks when I go to draw it into the syringe?
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
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Mateja


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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982298 - 10/12/20 08:08 PM (3 years, 3 months ago) |
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Yeah I was wondering why your LC was so tan also I was reading this discussion group for microbiology students where one guy is asking for tips on growth media with antibacterial properties that also promotes fungal growth and I learned that malt is one of the least suited ones for cultivating bacteria on so that's kind of nice
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Josex
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Yeah myc kinda "grabs" the sediment and your LC will clear up as it grows, taking that it's not contaminated.
In my pic you can see a dark spot, that's myc holding onto sediment. You gotta be careful with PC times for LME, there's always more sediment in my jars if I overcook them and they darken the fuck up too. Sediment has never been an issue for me though and it won't clog a needle.
Also, maybe this is just me but I seem to get less sediment if I mix the LME with very hot water instead of cold mixing it.
A 0.2% broth should come out almost as clear as water out of the PC if you did everything right. Like this:
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Becky G. Spot
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982313 - 10/12/20 08:12 PM (3 years, 3 months ago) |
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Quote:
Mateah said: ...I learned that malt is one of the least suited ones for cultivating bacteria on so that's kind of nice 
Quote:
Josex said: Yeah myc kinda "grabs" the sediment and your LC will clear up as it grows, taking that it's not contaminated.
In my pic you can see a dark spot, that's myc holding onto sediment. You gotta be careful with PC times for LME, there's always more sediment in my jars if I overcook them and they darken the fuck up too. Sediment has never been an issue for me though and it won't clog a needle.
Also, maybe this is just me but I seem to get less sediment if I mix the LME with very hot water instead of cold mixing it.
A 0.2% broth should come out almost as clear as water out of the PC if you did everything right. Like this:

Good to know!

XOXOX
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Josex
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Btw, when cooking LME broths, take them out of the PC as soon as the pressure dial hits zero. They'll come out darker too if you wait too long to take them out.
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Becky G. Spot
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982337 - 10/12/20 08:22 PM (3 years, 3 months ago) |
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Many thanks!
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
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van hatton
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Josex
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Re: Dem Chodes! GROWLOG. [Re: van hatton] 1
#26987268 - 10/15/20 03:07 PM (3 years, 3 months ago) |
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A little update from the first growlog.
Quote:
Josex said:
15 October.

We've got life in all the 4 little LC's. I started to stir them today, just a few minutes each.
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Dert
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#26987293 - 10/15/20 03:24 PM (3 years, 3 months ago) |
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lol
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Dert]
#26987297 - 10/15/20 03:29 PM (3 years, 3 months ago) |
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Heeey man! That's a nice lil white cock right there, you're so gay right now I know. Seriously, I'm so glad you pulled it off. Now it's my turn to try grow some cock. Welcome!
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#26988718 - 10/16/20 03:43 PM (3 years, 3 months ago) |
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16 October.
 
On the left, the 300ml LC I've been posting since the beginning and on the right, a 250ml LC (.2% LME and .1% agar) that I inoculated with the same clone 2 days after I inoculated the first LC.
I wasn't planning on making a second LC so I didn't even posted it. I don't know what I was thinking putting all the eggs in one basket for the growlog so I made the second LC as a backup in case something went wrong with the first one. And thank god I did because shit did indeed happen.
I was trying to aspirate the LC when I realized the tube was completely stuck. It almost never happens but it does happen sometimes and it had to happen for the growlog, right? 

Making the backup LC was not in the original plan but now that I had it I was planning on doing a side by side (regular LC versus agar LC) but now it's only going to be the agar LC, bummer. 
Have to say it's the first time I'm adding agar to an LC (was inspired by Nate's TEK, although I used half the agar he says in the tek) and was greatly surprised to see how thoroughly myc colonizes the broth with the help of a bit of agar. There were not uncolonized parts of liquid to be seen like it always happens with regular LC's and myc seems to colonize the broth faster too. The only drawback is that the agar clouds the liquid big time and I think it would be downright impossible to notice the usual visual signs of contamination in the LC.
Made some nice syringes and inoculated a bunch of jars of wheat.

I ordered the wheat online because the grain I can get locally is horrible stuff. But take a look at this online shit...

Doesn't look good at all and it's so difficult to hydrate. This is the best I could do.
 
I'm a little bummed with the grain situation but if this wheat doesn't work I will try another.
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gizmodo
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26989951 - 10/17/20 11:27 AM (3 years, 3 months ago) |
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Yeetusdeetus


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Re: Dem Chodes! GROWLOG. [Re: gizmodo]
#26989992 - 10/17/20 11:57 AM (3 years, 3 months ago) |
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How’d you hydrate em?
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Nichrome
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Doesn't look too bad. I've seen ugly grain produce wonderfully and pretty grain produce poorly. Just go for it.
-------------------- “Better to be deprived of food for three days, than tea for one.”
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filthyknees
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Re: Dem Chodes! GROWLOG. [Re: Nichrome]
#26990041 - 10/17/20 12:34 PM (3 years, 3 months ago) |
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Grain looks great, idk what you mean Jose. Cool thread
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Josex
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I guess we're about to find out, I hope you guys are right. I made a batch before prior to this one for other projects and they are colonizing rather slowly. This last batch that I'm using for the growlog seems better hydrated so I hope it works out.
Quote:
Yeetusdeetus said: How’d you hydrate em?
First attempt was a traditional prep, rinse-soak-boil-steam dry. And this last attempt was 24hrs soak with initial hot tap water-strain-load wet. This wheat seems to have a tougher outer layer than most I've had, maybe that has something to do.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26990163 - 10/17/20 02:23 PM (3 years, 3 months ago) |
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Better to have tough hulls than spilled starch lol
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Josex
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18 October.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26993373 - 10/19/20 05:47 PM (3 years, 3 months ago) |
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20 October.

These are 3 of the germination plates. All were inoculated with tiny pieces of the swab except the one in the first pic that got a big piece (you can actually see a piece of cotton sticking out if you enlarge the pic). Made the first transfers today and also made a couple LC's.
On another note, I'm a little disappointed on the 4 little 60ml LC's I started. They look good but they're taking more time than I was expecting.
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Yeetusdeetus


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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26993463 - 10/19/20 06:49 PM (3 years, 3 months ago) |
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When you use your blade to bury the swab do you twist and cover the cotton with loose puck or just do a quick in and out like when you use a needle?
Thinking of trying this with a couple new varieties, sounds a lot easier overall than a bunch of transfers
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Josex
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Quick in and out, maybe twist a little.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26993598 - 10/19/20 08:09 PM (3 years, 3 months ago) |
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Incidentally, now that I'm seeing the pics I think I made a not so good choice taking tranfers. I had really bad lightning fuck...
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tryptkaloids
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#26993803 - 10/19/20 10:57 PM (3 years, 3 months ago) |
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You seeing something im not? They look decent to me
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Josex
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Bad wording sorry, I was meaning to say that I think there were better looking sectors to take transfers from than those that I ended up poking.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#26998620 - 10/22/20 08:15 PM (3 years, 3 months ago) |
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From the first growlog:
Quote:
Josex said:
23 October.

Out of the four 60ml LC's this is the only one I stirred when I felt like it and it looked done today. First and last time I'm using these little jars, fuck em. I thought they were going to be done far sooner.

I aspirated 50ml of LC and inoculated 8 jars of wheat like the one below, which has all sort of shit mixed in that I didn't put there. 


One of the couple LC's I inoculated 3 days ago. It has .1% agar, hence the turbidity. I think I'm going to be adding agar to my LC's from now on, I'm liking it so far.
Ok so now both projects from the 2 growlogs are on grain. There's nothing to do but wait...
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#27000004 - 10/23/20 04:06 PM (3 years, 3 months ago) |
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You're putting quite the work in this thread, gotta hand it to ya, it's looking good with all those spinning gifs!
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Josex
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Appreciate it man. Really it doesn't feel like work, it's actually a lot of fun, dunno how I didn't do this before. It would feel like a lot of work if I had to write it up end to finish in one go, but that's the beauty of a live growlog, shit's slow and you only get to post up the little updates every once in a while.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27000119 - 10/23/20 05:22 PM (3 years, 3 months ago) |
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I also think you ought to submit one of those spinning gifs for a :liquidculture: graemlin... Just of course.
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Josex
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Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27000832 - 10/24/20 06:10 AM (3 years, 3 months ago) |
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O yeah that'll start a culture for sure! Even the minerals in non-demineralized water are enough for spores to hatch, just fyi.... That's why demineralized is used for spore syringes.
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Mateja


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coversall
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#27000850 - 10/24/20 06:36 AM (3 years, 3 months ago) |
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Holy shit I better take some notes. There's some mad things happening in here.
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Yeetusdeetus


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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#27000856 - 10/24/20 06:41 AM (3 years, 3 months ago) |
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--------------------

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seand04
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27000858 - 10/24/20 06:43 AM (3 years, 3 months ago) |
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Quote:
Josex said:

Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...

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Josex
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Re: Dem Chodes! GROWLOG. [Re: seand04] 1
#27007095 - 10/27/20 08:03 PM (3 years, 3 months ago) |
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Here's an update from the frist growlog:
Quote:
Josex said:
27 October.
 
These are the eight jars inoculated with the small LC four days ago. Time for a shake!

This is the 250ml LC (.2% LME and .1% agar) that was inoculated 7 days ago, but it was ready 2 days ago. The combination of stirring it and adding a bit of agar has shown to have a huge impact on the speed of colonization of the broth and it has also produced a substantial mycelial mass.

This is the LC resting. It won't settle because it can't settle. All the broth is colonized completely, top to bottom.
I inoculated 8 jars of wheat. There's a lot of leftover LC yet.

This one was inoculated the same day (200ml LC, .2% LME and .1% agar). This one didn't get stirred but was swirled a couple times a day instead. Not bad.
I inoculated another 8 jars with this one.
From the second:
Quote:
Josex said:
27 October.
 
This wheat is giving me shit as I expected. These are eleven days in and they're not done yet. Shook them at inoculation (I never do that, just wanted to check something out) and then shook again when the LC recovered, and they have received today what I hope will be the last shake.
I don't trust this wheat at all so I'm going to be making another LC with this clone and will use the same wheat I'm using for the first growlog. Regardless, I hope it works out because I inoculated all those jars you can see in the pic. Don't know what I was thinking going so big with a suspect grain.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27007412 - 10/27/20 11:09 PM (3 years, 2 months ago) |
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that second wheat looks kinda wack. maybe its better ground up for cakes or something.
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Re: Dem Chodes! GROWLOG. [Re: JHOVA]
#27007462 - 10/28/20 12:31 AM (3 years, 2 months ago) |
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Quote:
JHOVA said: second wheat looks kinda wack.
In scientific terms? 
@ajos3x I think I have to try to 0.1% agar as well and see what that does for the LC, great stuff
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#27007729 - 10/28/20 07:11 AM (3 years, 2 months ago) |
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Try it brudamang, it's seriously badass.
To me, grain can be wack for lots of reasons. In this case, myc is having a hell of time colonizing it. It's the same across different cultures, some of them were ready 20 days after inoculating with LC. I don't trust anything that takes that long, fuck it.
Whenever I'm trying a new bag of grain I'm always anxious. I've always had a lot of problems in the grain department. The wheat that is being used for the first growlog seems ok so far. Worth mentioning that I had to drive 40 minutes to buy it because the nearest feed stores only sell horrible stuff. Nonetheless, this 40-minutes-drive-wheat is still far from good and have all sorts of stuff mixed in.
Edited by Josex (10/28/20 08:36 AM)
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Mateja


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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27015776 - 11/01/20 01:36 PM (3 years, 2 months ago) |
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Yo I made 2 LC broths with 0.1%agar but I don't notice any change in the consistency of the solution, is it suppose to be visible? Or does it become more apparent when myc is colonizing? I don't see any effects of agar whatsoever in the LC is all
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
#27015816 - 11/01/20 02:02 PM (3 years, 2 months ago) |
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No? Well I did. Not only in the consistency, the liquid also gets way cloudier right from the start with that smidgen of agar. You can see the pics I have posted so far. Maybe my agar is stronger than yours.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27015823 - 11/01/20 02:06 PM (3 years, 2 months ago) |
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is it noticeable in the viscosity when swirling it? 1g/liter really shows itself ime, I could imagine .1 being visible when swirling as well.
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Josex
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Yeah it's visible and def noticeable when you swirl it. Myc colonizes the solution much faster too and more thoroughly.
Here you can see the difference between a regular LC (first one) and an agar LC (second one). 1gram agar to a liter of water.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27015835 - 11/01/20 02:16 PM (3 years, 2 months ago) |
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I've always liked adding agar at 1G/liter to lc's, I just never really bothered asking myself why other than it makes for nicer mycelium and a broth that will support pins
-------------------- The best things in life can be smelled on one's fingers.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Quadryder]
#27015851 - 11/01/20 02:23 PM (3 years, 2 months ago) |
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oh wait, I was thinking I was adding it at 1%, I'm adding at .1 as well 
definitely like the difference vs no agar as well.
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Josex
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Quote:
LizardWizard said: I've always liked adding agar at 1G/liter to lc's, I just never really bothered asking myself why other than it makes for nicer mycelium and a broth that will support pins 
You know what? I've always wanted to try adding a bit of agar to the LC but for reasons unknown never did it till now, weird in me because I'm always in for trying lots of shit. My 250ml LC's are colonizing in 5 days from biopsy without even using a stirrer, shit's crazy with that bit of agar.
Will spawn some jars tomorrow btw. And will be getting some seed quality grain soon, I'm so really tired of shit grain already.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27016650 - 11/02/20 01:29 AM (3 years, 2 months ago) |
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Oh there's one more very big convenience that I've noticed with the agar lc's. If you do your best not to let the inoculate sink below the surface, it will give you a 2D surface to start with, easier to judge cleanliness of transfer, then after it's growing you give it a swirl to spread inoculant through the whole medium.
-------------------- The best things in life can be smelled on one's fingers.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex] 2
#27018805 - 11/03/20 07:26 AM (3 years, 2 months ago) |
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Second growlog update:
Quote:
Josex said:
3 November.

These are some jars after a last recovery shake. I'm not happy how they're looking. Spawned only 4 jars to CVG (1:2) and made my first ez dialed tub ala Pasty.

Depending on how the spawn behaves in the next few days I'll decide whether or not to spawn the rest of the jars.
On another note,
Quote:
Josex said:

Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...

I dipped my inoc loop into both jars and streaked a couple agar plates. In one of them I got mold but the other jar tested clean.

I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27028933 - 11/08/20 04:51 PM (3 years, 2 months ago) |
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From the first Growlog,
Quote:
Josex said:
8 November.

These are the 8 jars inoculated with the mini LC after a recovery shake at 100%. Made a tub today, 4 qts of spawn to CVG (1:2). The reason I didn't spawn the other 4 jars is because I don't trust the wheat I got and I'm working with some millet now that I have a good feeling about, so I inoculated some jars of millet with an LC from one of the cultures from swabs that looked particularly good.

They were inoculated on 5 November and this pic was taken 72 hours after inoculation. Haven't had that recovery in a while. Myc seems to be loving the millet and if myc loves it I love it too. Gave 'em a shake today.
From the second,
Quote:
Josex said:
8 November.

This is the tub 5 days in. It isn't looking bad but it could look better and it gives off a slightly bacterial smell. I'm going to case it soon.
In the meantime, I'm still holding the rest of the jars in the pic. I'm still not liking the spawn and I'm not very happy how it's performing in the tub, so I may toss these eventually and make some millet spawn instead.

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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27033984 - 11/11/20 06:30 PM (3 years, 2 months ago) |
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11 November.

Cased it,

Since I can't seem to find plain peat without any additives, I've been using some potting soil for cactus for all my PE grows. For all of you having the same problem, know that you can use this. I always get such a nice coverage of the casing and very good surface conditions, myc loves it. The prep is the same as the usual 50/50 peat/verm casing, with lime and gypsum too. This is the stuff,
 
Also made a bucket with this clone today, 3qts to CVG (1:2). And another bucket for the first growlog which I won't bump for such a small update.

Also noc'd some millet jars today with the LS clone.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27044040 - 11/17/20 03:16 PM (3 years, 2 months ago) |
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From the first growlog,
17 November.
 
I let the millet jars consolidate a bit and spawned 3 of them to CVG (1:3).
I'm interested in seeing how the millet will do. I just wished I had used it from the beginning of the growlog, because the wheat I'm using is definitely sketch and I'm having problems with it with other grows. Well at least it's going to make for an interesting side by side, millet vs wack wheat.
Since my RH is pretty high I figured I could do with 3 extra holes at the top of each short side for the ez dial.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27061915 - 11/28/20 04:59 PM (3 years, 1 month ago) |
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Hey! I didn't quit or anything, it's just that penis matters go slow. Here's some little updates. Some stuff is about to happen.
First growlog,
Quote:
Josex said:
28 November.

Wheat tub with the casing mostly colonized. Me thinks we'll see pins in a matter of a few days.

The round wheat bucket getting there.
I have a bunch of tubs coming from different cultures that I got started from those 2 swabs I mentioned at the start of the growlog. Cultures looks very good on the PF-tek pucks and so aggressive they start to blob up right upon hitting 100% colonization, which I take it to be a really good sign.
Second,
Quote:
Josex said:
28 November.

I know it's nearly impossible to see them in the pic but we have the first pins starting to pop up. 
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27065763 - 12/01/20 04:37 AM (3 years, 1 month ago) |
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Quote:
Josex said:
On another note,
Quote:
Josex said:

Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...

I dipped my inoc loop into both jars and streaked a couple agar plates. In one of them I got mold but the other jar tested clean.

I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.

Found this little round phat guy today in the test plate. Guess who's gonna be cloned today.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27065814 - 12/01/20 06:04 AM (3 years, 1 month ago) |
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One of the tubs from swabs is side pinning like there's no tomorrow, no pins on the surface yet fml.
I attribute this to a few things I never do but did this time. The casing was way thicker than usual, colony said fuk u we're gonna pin right now and here ain't got time for that thick shit. New chamber, plastic is some thin shit that pulls from the sides leaving a huge gap on all 4 sides. And finally too much FAE too soon, should've taped some holes and wait for the casing to be colonized before cranking up FAE. I'm bummed.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27073832 - 12/05/20 04:34 PM (3 years, 1 month ago) |
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First GL,
Quote:
Josex said:
5 December.
 
This is a first for me shit's almost embarrassing, shrooms growing on the sides and nothing on the surface yet. This culture definitely didn't like that super thick casing. Too many things I tried for the first time here and too many things that went wrong as well.
But the game continues, have some more stuff from those swabs coming along from better spawn, all cased by now;
  
Second GL,
Quote:
Josex said:
5 December.

The pinset ain't precisely breathtaking but neither was the spawn, nonetheless I have the impression these fruits are going to be cool when grown out. I still have faith in this clone.
Here's a tub I cased recently, same clone;

Doesn't look like it right now but I promise you guys we're gonna see some killer stuff from these growlogs.
Edited by Josex (12/05/20 04:42 PM)
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EarthwormJim
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27073978 - 12/05/20 06:14 PM (3 years, 1 month ago) |
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We have faith in you bro
-------------------- Meddle not in the affairs of dragons for you are crunchy and good with ketchup Everything I post is a figment of someone's imagination. Maybe yours. Maybe mine. Likely both I see shrooms of blue In tubs of white That will keep me tripping All through the night And I think to myself What a wonderful world
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I think they're looking pretty cool right now, just about the phatest pins I've had with any PE variety. Me thinks is gonna be a tub of just a few giants.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#27078532 - 12/08/20 10:22 AM (3 years, 1 month ago) |
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8 December.

They have a long way to go yet. Yield's gonna be shitty but man aren't they purdy.
For reference, this is a 60L tub.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27084487 - 12/11/20 06:16 PM (3 years, 1 month ago) |
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11 December.

First tub of the LS clon done.
Some 3 digit monsters:
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mushboy
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27084509 - 12/11/20 06:26 PM (3 years, 1 month ago) |
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Damn dude those some chubbins
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Pastywhyte
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
#27084519 - 12/11/20 06:30 PM (3 years, 1 month ago) |
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Yeah them some real chodes
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Josex
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
#27084522 - 12/11/20 06:31 PM (3 years, 1 month ago) |
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I've never had such chunkers, just threw them in the drator whole. Should I cut them in smaller pieces or something?
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mushboy
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#27084528 - 12/11/20 06:34 PM (3 years, 1 month ago) |
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The ladies like em whole
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Wall.E
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
#27084571 - 12/11/20 06:58 PM (3 years, 1 month ago) |
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You better eat the whole thing
-------------------- Life’s shit, but I’m loving it
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sh4d0ws
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27084732 - 12/11/20 08:51 PM (3 years, 1 month ago) |
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Quote:
Josex said: I've never had such chunkers, just threw them in the drator whole. Should I cut them in smaller pieces or something? 
Cutting them up definitely reduces drying time. You could just slice the stems up the center and leave em mostly in tact, still well help drying speed.
I do just leave mine whole for bag appeal but honestly at 36 hours of drying times on the fatter ones just means my dehydrator will eventually burn out faster. Had my nesco burn out recently. Hoping I can repair it...still waiting on thermal fuses though.
always glad to see those spores ended up doing well for you... I have a fat ass clone I am gonna put to grain next week I hope it puts out something like you got
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Re: Dem Chodes! GROWLOG. [Re: sh4d0ws]
#27084745 - 12/11/20 09:00 PM (3 years, 1 month ago) |
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And this is the guy who gave me the whole PE syringe I've been having fun with all this time, the same syringe that made possible this growlog. SW sold me a dead syringe and wouldn't send me a replacent or a refund (cuz they're for mycroscopy use only and that kinda shit) so this guy came out of the blue and sent me a syringe for free.
I think imma cut them in half tomorrow, otherwise they're going to take forever.
Edited by Josex (12/11/20 09:42 PM)
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27094461 - 12/17/20 03:51 PM (3 years, 1 month ago) |
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Some little updates from the first GL,
Quote:
17 December.
 
I've got several tubs that are blobbing right now, no pins. Fortunately I have 2 millet tubs from a different swab culture that are starting to pin.

This is the side pinning bastard today. Shitty grain, shitty prep and shitty spawn, there's little more to it. I'm not tossing this culture just yet because it's putting out some cool looking monsters and want to see what it can do with better spawn.
Same culture, same spawn, being weird and blobbing a bit in a bucket:

Second,
Quote:
17 December.

Here's the LS clone on millet spawn. I think we're going to see pins very soon.
Was thinking today that it looks like this growlog it's going to be really fucking long. I'm planning on fruiting all the 7 cultures I have from swabs and the clone I took from that test plate I posted the other day, of which I have an LC almost ready to roll.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27099409 - 12/20/20 02:17 PM (3 years, 1 month ago) |
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Quote:
20 December.
 
Two different tubs of culture #5 from swab. These might turn out nice I think.

Culture #1 blobbing all over the place.

Culture #3, which is the same one from the tub I posted earlier which put out some 3 digit stuff. Pics after harvest yesterday;
 
There's something so satisfying about growing PE from your own swabs. Highly recommend.
Edited by Josex (12/20/20 02:23 PM)
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1kldude
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#27099482 - 12/20/20 02:55 PM (3 years, 1 month ago) |
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Congrats on the 100g+ fruits!
--------------------
<<-----|||1kldude|||----->> <<<----------|||1 knowledgeable dude|||---------->>> <<<<----Any post I make is purely fictional and for informational purposes only---->>>>
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Inthepit
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27099746 - 12/20/20 06:01 PM (3 years, 1 month ago) |
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Oh boy, that looks really difficult. Keep the grow log going... I appreciate how clear your writing is.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Inthepit]
#27102080 - 12/22/20 06:48 AM (3 years, 1 month ago) |
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Quote:
22 December.
 
Dem peens today. At least we know culture #5 delivers. Going to clone something from that clusterfuck when the tubs are ready.

Culture #3 (the same culture as the tub posted earlier that was side pinning). Seems to keep pulling out some phaties. I want to see if the bucket can produce some 3 digit stuff too.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27106255 - 12/24/20 05:29 PM (3 years, 1 month ago) |
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Quote:
24 December.

In these LC's are the last of the cultures that were still waiting their turn to be fruited out. Culture #6 looks amazing too but I just didn't have a free LC jar for it, so that one it's going to be last to be fruited.
From each jar I inoculated 5 jars of barley spawn today.
If anyone is wondering what the hell is "cloneT", here's the story:
Quote:
Josex said:
Quote:
Josex said:
On another note,
Quote:
Josex said:

Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. 
Just now I was going through my old plates to see what I could toss and found these. I think there's enough nutes there for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.
Life finds a way...

I dipped my inoc loop into both jars and streaked a couple agar plates. In one of them I got mold but the other jar tested clean.

I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.

Found this little round phat guy today in the test plate. Guess who's gonna be cloned today.

Will have some cool tub pics tomorrow or next.
Happy Christmas people
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27109300 - 12/26/20 04:18 PM (3 years, 1 month ago) |
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26 December.
Culture #5, tub 1:
 
The chunkiest of the lot:

Final weight from tub 1, can't complain from MS and 3 quarts of millet:

Culture #5, tub 2 which I could've let it ride a bit longer (was spawned 2 days after tub 1), but needed to harvest today:
 
The chunkiest of the lot:
 
Final weight from tub 2:

Culture #3, bucket:

Sum crazy shit:
    
Culture #1, decided it was time to pin after all those blobs:
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mushboy
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27109672 - 12/26/20 08:05 PM (3 years, 1 month ago) |
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that bucket is awesome. the zippo is so tiny
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: mushboy] 1
#27111458 - 12/27/20 11:10 PM (3 years, 1 month ago) |
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You should hand Mateah that 200g one so he can play Jebus n break "bread" with his 12 close friends.
-------------------- The best things in life can be smelled on one's fingers.
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Josex
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Haha that was funny to picture in my mind.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27115988 - 12/30/20 10:46 AM (3 years, 29 days ago) |
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I'd send him a mushroom butt plug gag gift as a means of telling him not to take things so seriously, but he's already too butthurt.
Been browsing glowfuckyourself.com a bit too much.
-------------------- The best things in life can be smelled on one's fingers.
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LizardWizard
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Fuck me, this one is just too damn funny
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Josex
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Quote:
4 January.
Time to harvest culture #1 from the bucket:

Some moar freaks, it's not even funny anymore:

Mutant family


I'm not even sure if these are safe to eat 
Current tale of the tape:
- Culture #5: keeper.
- Culture #3: worth giving another try imo.
- Culture #1: would like to keep it for weird but it's terribly slow and yields poorly.
Coming next cultures: #2, #4 and #7. Culture #6 will be last to be grown.
Edited by Josex (01/04/21 02:16 PM)
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27132569 - 01/07/21 10:05 AM (3 years, 21 days ago) |
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Quote:
I'm not even sure if these are safe to eat 
As long as you chew them up good enough so they can't haunt you from the other side, you'll be good!
After loosening the lid on my PF puck, I thought there was no progresion, so I loosened it just a tad more and then marked the edges of the mycelium with a sharpie. That was 2 nights ago. It seems there's 2-3mm new growth in the puck now. That sounds about right for a case of bad GE, right?
-------------------- The best things in life can be smelled on one's fingers.
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Josex
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It's choked I'd go ahead and punch a hole in front of your FH and put 2-3 layers of mp tape.
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LizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27132727 - 01/07/21 11:24 AM (3 years, 21 days ago) |
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It for sure is 
I think if I just open it up in front of the hood and close it back up it'll have enough to finish the puck off, it's really just the margin 
If not I'll do a repeat this weekend. Turning on the hood rn.
-------------------- The best things in life can be smelled on one's fingers.
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LizardWizard
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Ok, so after opening it up and closing it back up again, I left it for a couple days, yesterday the mycelium had eaten through almost all of the PF puck, so I cracked it ever so slightly again, right now it's looking like it'll be ready to open up tomorrow morning.
PE takes a long time to fruit, right?
I'm thinking of doing a dunk about 10 days from now..?
Then afterwards put it under some bright lighting to initiate pinning.
Man, I'm completely out of the game.
-------------------- The best things in life can be smelled on one's fingers.
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Josex
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Honestly I haven't been able to get them to fruit on pucks, only that one clone out of bunch of pucks that is the one featured in the second growlog that was cased with plain verm.
They just love to blob on pucks, nothing else. Sure blobs carry fruiting genetics but you never know what you'll get if you clone them. I don't think taking clones from pucks is such a good idea with PE anyway, the conditions in a tub are wildly different so I prefer to clone actual fruits from tubs so I can easily replicate those same conditions for more consistent results.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27143990 - 01/12/21 02:24 PM (3 years, 16 days ago) |
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Second growlog, (bad) news from yesterday but posted today:
Quote:
11 January.
So the tub looked and smelled fine before casing it and after casing it too (at first) but something weird happened:
 
The sparse few fruits took too long to pop up and growth is obviously stunted. There's also some fuzz on the caps that looks like some kind of mold, just kept it this long out of curiosity to see what'd do.
These last days the tub was giving off an earthy smell so I was expecting trich at any moment. No trich but some kind of mold started to pop up on the surface (circled in red in the pic), so I had to toss the tub. Broke the sub apart to inspect and it looked and smelled fine. The earthy smell seemed to come from the casing only.
Same issue with the same clone, this time in a bucket:

 
I'm suspecting the casing. I got lazy and used some jars with casing material that had been sitting for way over a month on a shelf after pasteurization. Had the exact same issue with a tub of KSSS that also got cased with the same old casing material.
These tubs were looking great from the beginning so I was stoked to see how they'd perform from what I thought was better spawn than the spawn used for the previous grow. Guess I'll have to start over.
If someone knows what's that on the caps, please chime in I'm curious, it certainly didn't look like cube fuzz at all irl.
Edited by Josex (01/13/21 06:02 AM)
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Wall.E
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Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#27144222 - 01/12/21 05:04 PM (3 years, 16 days ago) |
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Gonorrhea
-------------------- Life’s shit, but I’m loving it
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Wall.E]
#27144239 - 01/12/21 05:15 PM (3 years, 16 days ago) |
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27174335 - 01/28/21 10:28 AM (3 years, 10 hours ago) |
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The growlog is getting long so I opted to cut to the chase and post pics of the tubs only, here's the update:
Quote:
28 January.
Culture #7 (spawned on 1/13), colonizing the casing aggressively:

Culture #4 (spawned on 1/10), this one likes it slower:

Culture #2 (spawned on 1/13):

Also, just inoculated a bunch of jars with culture #6 to finish the growlog.
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Josex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27221365 - 02/23/21 12:42 AM (2 years, 10 months ago) |
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Culture #2, triched out before first flush.
Culture #4, threw out a bunch blobs and then triched out shortly after taking the pic:

Culture #7, started blobbing a bit, then decided to pin, then I saw a spot of trich at the bottom of the tub, tossed it...

Shit grain (barley this time) is the name of the game. Since then I've done some adjustments to hopefully stop this from happening again.
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Re: Dem Chodes! GROWLOG. [Re: Josex]
#27223866 - 02/24/21 11:59 AM (2 years, 10 months ago) |
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Well that sucks.
Plates of blob tissue doing alright. Recovered within a day. Made 4 plates.
-------------------- The best things in life can be smelled on one's fingers.
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