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Offlineseand04
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27000858 - 10/24/20 06:43 AM (3 years, 3 months ago)

Quote:

Josex said:


Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. :cool:

Just now I was going through my old plates to see what I could toss and found  these. I think there's enough nutes there  for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.

Life finds a way...
:Awemazing:



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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: seand04] * 1
    #27007095 - 10/27/20 08:03 PM (3 years, 3 months ago)

Here's an update from the frist growlog:

Quote:

Josex said:


27 October.



These are the eight jars inoculated with the small LC four days ago. Time for a shake!



This is the 250ml LC (.2% LME and .1% agar) that was inoculated 7 days ago, but it was ready 2 days ago. The combination of stirring it and adding a bit of agar has shown to have a huge impact on the speed of colonization of the broth and it has also produced a substantial mycelial mass.



This is the LC resting. It won't settle because it can't settle. All the broth is colonized completely, top to bottom.

I inoculated 8 jars of wheat. There's a lot of leftover LC yet.



This one was inoculated the same day (200ml LC, .2% LME and .1% agar). This one didn't get stirred but was swirled a couple times a day instead. Not bad.

I inoculated another 8 jars with this one.




From the second:

Quote:

Josex said:


27 October.



This wheat is giving me shit as I expected.
These are eleven days in and they're not done yet. Shook them at inoculation (I never do that, just wanted to check something out) and then shook again when the LC recovered, and they have received today what I hope will be the last shake.

I don't trust this wheat at all so I'm going to be making another LC with this clone and will use the same wheat I'm using for the first growlog.
Regardless, I hope it works out because I inoculated all those jars you can see in the pic. Don't know what I was thinking going so big with a suspect grain.




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InvisibleJHOVA
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27007412 - 10/27/20 11:09 PM (3 years, 2 months ago)

:camping:

that second wheat looks kinda wack. maybe its better ground up for cakes or something.


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InvisibleMateja
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Re: Dem Chodes! GROWLOG. [Re: JHOVA]
    #27007462 - 10/28/20 12:31 AM (3 years, 2 months ago)

Quote:

JHOVA said:
second wheat looks kinda wack.



In scientific terms? :begger:


@ajos3x I think I have to try to 0.1% agar as well and see what that does for the LC, great stuff :popcorn:


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Cakes inside Water Tub


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
    #27007729 - 10/28/20 07:11 AM (3 years, 2 months ago)

Try it brudamang, it's seriously badass.

To me, grain can be wack for lots of reasons. In this case, myc is having a hell of time colonizing it. It's the same across different cultures, some of them were ready 20 days after inoculating with LC. I don't trust anything that takes that long, fuck it.

Whenever I'm trying a new bag of grain I'm always anxious. I've always had a lot of problems in the grain department.
The wheat that is being used for the first growlog seems ok so far.
Worth mentioning that I had to drive 40 minutes to buy it because the nearest feed stores only sell horrible stuff. Nonetheless, this 40-minutes-drive-wheat is still far from good and have all sorts of stuff mixed in.


Edited by Josex (10/28/20 08:36 AM)


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InvisibleMateja
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27015776 - 11/01/20 01:36 PM (3 years, 2 months ago)

Yo I made 2 LC broths with 0.1%agar but I don't notice any change in the consistency of the solution, is it suppose to be visible? Or does it become more apparent when myc is colonizing? I don't see any effects of agar whatsoever in the LC is all :shrug:


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Mateja]
    #27015816 - 11/01/20 02:02 PM (3 years, 2 months ago)

No? Well I did. Not only in the consistency, the liquid also gets way cloudier right from the start with that smidgen of agar. You can see the pics I have posted so far. Maybe my agar is stronger than yours.


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InvisibleLizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27015823 - 11/01/20 02:06 PM (3 years, 2 months ago)

is it noticeable in the viscosity when swirling it? 1g/liter really shows itself ime, I could imagine .1 being visible when swirling as well.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: LizardWizard]
    #27015828 - 11/01/20 02:09 PM (3 years, 2 months ago)

Yeah it's visible and def noticeable when you swirl it. Myc colonizes the solution much faster too and more thoroughly.

Here you can see the difference between a regular LC (first one) and an agar LC (second one). 1gram agar to a liter of water.


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InvisibleLizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27015835 - 11/01/20 02:16 PM (3 years, 2 months ago)

I've always liked adding agar at 1G/liter to lc's, I just never really bothered asking myself why other than it makes for nicer mycelium and a broth that will support pins :lol:


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Re: Dem Chodes! GROWLOG. [Re: LizardWizard]
    #27015840 - 11/01/20 02:20 PM (3 years, 2 months ago)

:threadmonitor:


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InvisibleLizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Quadryder]
    #27015851 - 11/01/20 02:23 PM (3 years, 2 months ago)

oh wait, I was thinking I was adding it at 1%, I'm adding at .1 as well :facepalm:

definitely like the difference vs no agar as well.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: LizardWizard]
    #27015853 - 11/01/20 02:26 PM (3 years, 2 months ago)

Quote:

LizardWizard said:
I've always liked adding agar at 1G/liter to lc's, I just never really bothered asking myself why other than it makes for nicer mycelium and a broth that will support pins :lol:




You know what? I've always wanted to try adding a bit of agar to the LC but for reasons unknown never did it till now, weird in me because I'm always in for trying lots of shit. My 250ml LC's are colonizing in 5 days from biopsy without even using a stirrer, shit's crazy with that bit of agar.

Will spawn some jars tomorrow btw. And will be getting some seed quality grain soon, I'm so really tired of shit grain already.


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InvisibleLizardWizard
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27016650 - 11/02/20 01:29 AM (3 years, 2 months ago)

Oh there's one more very big convenience that I've noticed with the agar lc's. If you do your best not to let the inoculate sink below the surface, it will give you a 2D surface to start with, easier to judge cleanliness of transfer, then after it's growing you give it a swirl to spread inoculant through the whole medium.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex] * 2
    #27018805 - 11/03/20 07:26 AM (3 years, 2 months ago)

Second growlog update:

Quote:

Josex said:

3 November.



These are some jars after a last recovery shake. I'm not happy how they're looking. Spawned only 4 jars to CVG (1:2) and made my first ez dialed tub ala Pasty.



Depending on how the spawn behaves in the next few days I'll decide whether or not to spawn the rest of the jars.





On another note,

Quote:

Josex said:


Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. :cool:

Just now I was going through my old plates to see what I could toss and found  these. I think there's enough nutes there  for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.

Life finds a way...
:Awemazing:




I dipped my inoc loop into both jars and streaked a couple agar plates.
In one of them I got mold but the other jar tested clean.



I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27028933 - 11/08/20 04:51 PM (3 years, 2 months ago)

From the first Growlog,

Quote:

Josex said:


8 November.



These are the 8 jars inoculated with the mini LC after a recovery shake at 100%. Made a tub today, 4 qts of spawn to CVG (1:2). The reason I didn't spawn the other 4 jars is because I don't trust the wheat I got and I'm working with some millet now that I have a good feeling about, so I inoculated some jars of millet with an LC from one of the cultures from swabs that looked particularly good.



They were inoculated on 5 November and this pic was taken 72 hours after inoculation. Haven't had that recovery in a while. Myc seems to be loving the millet and if myc loves it I love it too. Gave 'em a shake today.





From the second,

Quote:

Josex said:


8 November.



This is the tub 5 days in. It isn't looking bad but it could look better and it gives off a slightly bacterial smell. I'm going to case it soon.

In the meantime, I'm still holding the rest of the jars in the pic. I'm still not liking the spawn and I'm not very happy how it's performing in the tub, so I may toss these eventually and make some millet spawn instead.






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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27033984 - 11/11/20 06:30 PM (3 years, 2 months ago)

11 November.



Cased it,



Since I can't seem to find plain peat without any additives, I've been using some potting soil for cactus for all my PE grows.
For all of you having the same problem, know that you can use this.
I always get such a nice coverage of the casing and very good surface conditions, myc loves it. The prep is the same as the usual 50/50 peat/verm casing, with lime and gypsum too.
This is the stuff,



Also made a bucket with this clone today, 3qts to CVG (1:2). And another bucket for the first growlog which I won't bump for such a small update.



Also noc'd some millet jars today with the LS clone.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27044040 - 11/17/20 03:16 PM (3 years, 2 months ago)

From the first growlog,


17 November.



I let the millet jars consolidate a bit and spawned 3 of them to CVG (1:3).

I'm interested in seeing how the millet will do. I just wished I had used it from the beginning of the growlog, because the wheat I'm using is definitely sketch and I'm having problems with it with other grows.
Well at least it's going to make for an interesting side by side, millet vs wack wheat.

Since my RH is pretty high I figured I could do with 3 extra holes at the top of each short side for the ez dial.


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27061915 - 11/28/20 04:59 PM (3 years, 1 month ago)

Hey! I didn't quit or anything, it's just that penis matters go slow. Here's some little updates. Some stuff is about to happen.

First growlog,

Quote:

Josex said:


28 November.



Wheat tub with the casing mostly colonized. Me thinks we'll see pins in a matter of a few days.



The round wheat bucket getting there.

I have a bunch of tubs coming from different cultures that I got started from those 2 swabs I mentioned at the start of the growlog. Cultures looks very good on the PF-tek pucks and so aggressive they start to blob up right upon hitting 100% colonization, which I take it to be a really good sign.




Second,

Quote:

Josex said:



28 November.



I know it's nearly impossible to see them in the pic but we have the first pins starting to pop up. :popcorn:





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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #27065763 - 12/01/20 04:37 AM (3 years, 1 month ago)

Quote:

Josex said:

On another note,

Quote:

Josex said:


Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. :cool:

Just now I was going through my old plates to see what I could toss and found  these. I think there's enough nutes there  for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.

Life finds a way...
:Awemazing:




I dipped my inoc loop into both jars and streaked a couple agar plates.
In one of them I got mold but the other jar tested clean.



I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.






Found this little round phat guy today in the test plate. Guess who's gonna be cloned today.

:hereyougo:


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