|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Dem Chodes! GROWLOG. [Re: LemonTekno]
#26981834 - 10/12/20 04:06 PM (3 years, 3 months ago) |
|
|
Some updates. Brace yerselves for the WEIRDNESS...
From the first growlog:
Quote:
Josex said:
12 October.

It's really hard to take a good pic through the glass. All the pucks have some beautiful rhizo growth, pics don't make it justice.
Growth looks healthy enough for me so I decided to do something with them pucks. I took needle biopsies from 4 pucks and inoculated 4 little LC jars.

I know right?
If my regular LC jar are somewhat ridiculous, those above are just too much, I know. Made them 2 years ago as "LC starter" jars (to inoculate LC with LC), but this is actually the first time I'm using them.
I know you perverts want to see some cock asap so that's why I decided to use these. In each jar there's only 60ml of broth (.25% LME) and also threw a stir bar in each to speed things up, so I'm gonna be taking the dust off my magnetic stirrer for this one as soon as I see the first tiny puffs of myc in the liquid.
In case someone is wondering how the fuck I inoculated the jars, I just stuck the needle through that sophisticated self healing injection port called polyfil.

From the second growlog:
Quote:
Josex said:
12 October.

That's the 300ml LC eight days in. I could use it right now but I'd rather let it go a bit longer so there's more myc. I think it's gonna be ready in 5-6 days.
Edited by Josex (10/12/20 04:19 PM)
|
Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
|
Re: Dem Chodes! GROWLOG. [Re: Josex] 2
#26981845 - 10/12/20 04:13 PM (3 years, 3 months ago) |
|
|
I saw in another thread you were telling some guys that you were going to post some "new and improved" LC lid build design. Did that ever happen? I would love to see it if so.
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
|
No, I'm onto it though. Currently waiting for the parts that I ordered more than a month ago. This covid BS is really slowing things down.
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982081 - 10/12/20 06:21 PM (3 years, 3 months ago) |
|
|
Chillin here just because of your little jab
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
|
Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982119 - 10/12/20 06:51 PM (3 years, 3 months ago) |
|
|
Quote:
Josex said:

That's the 300ml LC eight days in.
Homogenous in growth appearance tells me that there's only one type of filamentous organism in there. The lack of turbidity implies that there's no microbial activity present in the uncolonized parts of the broth. I like where this is going
-------------------- Cakes inside Water Tub
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 58 minutes, 13 seconds
|
Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982159 - 10/12/20 07:16 PM (3 years, 3 months ago) |
|
|
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982242 - 10/12/20 07:47 PM (3 years, 3 months ago) |
|
|
Quote:
Mateah said:
Quote:
Josex said:

That's the 300ml LC eight days in.
Homogenous in growth appearance tells me that there's only one type of filamentous organism in there. The lack of turbidity implies that there's no microbial activity present in the uncolonized parts of the broth. I like where this is going 
Yeah I think it looks good too. My dumbass didn't hear the timer and cooked that LC ten minutes more than usual, that's why it's so dark, LME is delicate like that. It was a .3% LME broth, a tad more than usual, but it should have come out way clearer.
|
Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982253 - 10/12/20 07:51 PM (3 years, 3 months ago) |
|
|
I did a 0.2 concentration of LME and got TONS of sediment.
Why?
I am worried it's gonna clog the needle. Will the myc devour it? Or does it just attach itself to the outside of said sediment, and I am going to have to contend with chunks when I go to draw it into the syringe?
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
|
Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982298 - 10/12/20 08:08 PM (3 years, 3 months ago) |
|
|
Yeah I was wondering why your LC was so tan also I was reading this discussion group for microbiology students where one guy is asking for tips on growth media with antibacterial properties that also promotes fungal growth and I learned that malt is one of the least suited ones for cultivating bacteria on so that's kind of nice
-------------------- Cakes inside Water Tub
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
|
Yeah myc kinda "grabs" the sediment and your LC will clear up as it grows, taking that it's not contaminated.
In my pic you can see a dark spot, that's myc holding onto sediment. You gotta be careful with PC times for LME, there's always more sediment in my jars if I overcook them and they darken the fuck up too. Sediment has never been an issue for me though and it won't clog a needle.
Also, maybe this is just me but I seem to get less sediment if I mix the LME with very hot water instead of cold mixing it.
A 0.2% broth should come out almost as clear as water out of the PC if you did everything right. Like this:
|
Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
|
Re: Dem Chodes! GROWLOG. [Re: Mateja]
#26982313 - 10/12/20 08:12 PM (3 years, 3 months ago) |
|
|
Quote:
Mateah said: ...I learned that malt is one of the least suited ones for cultivating bacteria on so that's kind of nice 
Quote:
Josex said: Yeah myc kinda "grabs" the sediment and your LC will clear up as it grows, taking that it's not contaminated.
In my pic you can see a dark spot, that's myc holding onto sediment. You gotta be careful with PC times for LME, there's always more sediment in my jars if I overcook them and they darken the fuck up too. Sediment has never been an issue for me though and it won't clog a needle.
Also, maybe this is just me but I seem to get less sediment if I mix the LME with very hot water instead of cold mixing it.
A 0.2% broth should come out almost as clear as water out of the PC if you did everything right. Like this:

Good to know!

XOXOX
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
|
Btw, when cooking LME broths, take them out of the PC as soon as the pressure dial hits zero. They'll come out darker too if you wait too long to take them out.
|
Becky G. Spot
Good Necky Becky

Registered: 10/12/20
Posts: 111
Loc: The Sunny Side
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26982337 - 10/12/20 08:22 PM (3 years, 3 months ago) |
|
|

Many thanks!
-------------------- - Becky G. Ditch the double standards boys. Girls like to have fun as much as you do.
Sisters if you find yourself hating, read my bio.
|
van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
|
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Dem Chodes! GROWLOG. [Re: van hatton] 1
#26987268 - 10/15/20 03:07 PM (3 years, 3 months ago) |
|
|
A little update from the first growlog.
Quote:
Josex said:
15 October.

We've got life in all the 4 little LC's. I started to stir them today, just a few minutes each.
|
Dert
Stranger



Registered: 10/10/20
Posts: 32
Last seen: 1 year, 10 days
|
Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#26987293 - 10/15/20 03:24 PM (3 years, 3 months ago) |
|
|
lol
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Dem Chodes! GROWLOG. [Re: Dert]
#26987297 - 10/15/20 03:29 PM (3 years, 3 months ago) |
|
|
Heeey man! That's a nice lil white cock right there, you're so gay right now I know. Seriously, I'm so glad you pulled it off. Now it's my turn to try grow some cock. Welcome!
|
Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
|
Re: Dem Chodes! GROWLOG. [Re: Josex] 1
#26988718 - 10/16/20 03:43 PM (3 years, 3 months ago) |
|
|
16 October.
 
On the left, the 300ml LC I've been posting since the beginning and on the right, a 250ml LC (.2% LME and .1% agar) that I inoculated with the same clone 2 days after I inoculated the first LC.
I wasn't planning on making a second LC so I didn't even posted it. I don't know what I was thinking putting all the eggs in one basket for the growlog so I made the second LC as a backup in case something went wrong with the first one. And thank god I did because shit did indeed happen.
I was trying to aspirate the LC when I realized the tube was completely stuck. It almost never happens but it does happen sometimes and it had to happen for the growlog, right? 

Making the backup LC was not in the original plan but now that I had it I was planning on doing a side by side (regular LC versus agar LC) but now it's only going to be the agar LC, bummer. 
Have to say it's the first time I'm adding agar to an LC (was inspired by Nate's TEK, although I used half the agar he says in the tek) and was greatly surprised to see how thoroughly myc colonizes the broth with the help of a bit of agar. There were not uncolonized parts of liquid to be seen like it always happens with regular LC's and myc seems to colonize the broth faster too. The only drawback is that the agar clouds the liquid big time and I think it would be downright impossible to notice the usual visual signs of contamination in the LC.
Made some nice syringes and inoculated a bunch of jars of wheat.

I ordered the wheat online because the grain I can get locally is horrible stuff. But take a look at this online shit...

Doesn't look good at all and it's so difficult to hydrate. This is the best I could do.
 
I'm a little bummed with the grain situation but if this wheat doesn't work I will try another.
|
gizmodo
Woodland Creature




Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
|
Re: Dem Chodes! GROWLOG. [Re: Josex]
#26989951 - 10/17/20 11:27 AM (3 years, 3 months ago) |
|
|
-------------------- One must be mindful to maintain their balance on the slippery earth. Bod's Comprehensive Agar Resource Gizmodo's Market Stall Say No To Grow Kits
|
Yeetusdeetus


Registered: 11/23/19
Posts: 1,242
Last seen: 4 days, 3 hours
|
Re: Dem Chodes! GROWLOG. [Re: gizmodo]
#26989992 - 10/17/20 11:57 AM (3 years, 3 months ago) |
|
|
How’d you hydrate em?
--------------------

|
|