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InvisibleJosex
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Dem Dicks! GROWLOG. * 3
    #26968160 - 10/03/20 08:03 PM (3 years, 3 months ago)

A GROW FROM SWABS


:wave:

Never made a growlog before but suddenly got the itch so here it is.

Very recently I finished some MS PE grows from a PE syringe (SW) that was gifted to me by an awesome member. They had a special look to them so I felt I had to take some swabs. Some pics I posted in POTD:



I took some nice dark swabs from these fruits:


So without further ado, let's get the show on the road!


4 October.


I used 2 swabs to inoculate those 5 PF-tek pucks in the pic. You can learn more about them here.

To inoculate the pucks I did the following procedure (from the tek above) that has worked really well for me in the past:

Quote:

Josex said:
  • Use a little filtered container to sterilize enough water to cover the bottom half an inch.
  • In the SAB, take the swab and dunk the tip in the sterile water a few secs to soften up the cotton fibers and to rinse it off a bit.
  • Take your scalpel and use the tip of the blade to remove a tiny, spore-laden piece of cotton from the swab.
  • Bury the piece of cotton in the PF-tek puck.




Now the waiting game, will be updating this regurlarly. :cheers:


7 October.



Woke up today to some germination on 3 plates out of 5, nice. It's difficult to see in the pic but we have some dick myc right in the middle.


12 October.



It's really hard to take a good pic through the glass. All the pucks have some beautiful rhizo growth, pics don't make it justice.

Growth looks healthy enough for me so I decided to do something with them pucks. I took needle biopsies from 4 pucks and inoculated 4 little LC jars.



I know right?  :smugjerry:

If my regular LC jars are somewhat ridiculous, those above are just too much, I know. Made them 2 years ago as "LC starter" jars (to inoculate LC with LC), but this is actually the first time I'm using them.

I know you perverts want to see some cock asap so that's why I decided to use these.
In each jar there's only 60ml of broth (.25% LME) and also threw a stir bar in each to speed things up, so I'm gonna be taking the dust off my magnetic stirrer for this one as soon as I see the first tiny puffs of myc in the liquid.

In case someone is wondering how the fuck I inoculated the jars, I just stuck the needle through that sophisticated self healing injection port called polyfil.




15 October.



We've got life in all the 4 little LC's. I started to stir them today, just a few minutes each.



18 October.




20 October.



These are 3 of the germination plates. All were inoculated with tiny pieces of the swab except the one in the first pic that got a big piece (you can actually see a piece of cotton sticking out if you enlarge the pic). Made the first transfers today and also made a couple LC's.

On another note, I'm a little disappointed on the 4 little 60ml LC's I started. They look good but they're taking more time than I was expecting.


23 October.



Out of the four 60ml LC's this is the only one I stirred when I felt like it and it looked done today. First and last time I'm using these little jars, fuck em. I thought they were going to be done far sooner.



I aspirated 50ml of LC and inoculated 8 jars of wheat like the one below, which has all sorts of shit mixed in that I didn't put there. :lol:





One of the couple LC's I inoculated 3 days ago. It has .1% agar, hence the turbidity. I think I'm going to be adding agar to my LC's from now on, I'm liking it so far.


27 October.



These are the eight jars inoculated with the small LC four days ago. Time for a shake!



This is the 250ml LC (.2% LME and .1% agar) that was inoculated 7 days ago, but it was ready 2 days ago. The combination of stirring it and adding a bit of agar has shown to have a huge impact on the speed of colonization of the broth and it has also produced a substantial mycelial mass.



This is the LC resting. It won't settle because it can't settle. All the broth is colonized completely, top to bottom.
I inoculated 8 jars of wheat. There's a lot of leftover LC yet.



This one was inoculated the same day (200ml LC, .2% LME and .1% agar). This one didn't get stirred but was swirled a couple times a day instead. Not bad.
I inoculated another 8 jars with this one.


8 November.



These are the 8 jars inoculated with the mini LC after a recovery shake at 100%. Made a tub today, 4 qts of spawn to CVG (1:2). The reason I didn't spawn the other 4 jars is because I don't trust the wheat I got and I'm working with some millet now that I have a good feeling about, so I inoculated some jars of millet with an LC from one of the cultures from swabs that looked particularly good.



They were inoculated on November 5 and this pic was taken 72 hours after inoculation. Haven't had that recovery in a while. Myc seems to be loving the millet and if myc loves it I love it too. Gave 'em a shake today.


17 November.



I let the millet jars consolidate a bit and spawned 3 of them to CVG (1:3).

I'm interested in seeing how the millet will do. I just wished I had used it from the beginning of the growlog, because the wheat I'm using is definitely sketch and I'm having problems with it with other grows.
Well at least it's going to make for an interesting side by side, millet vs wack wheat.

Since my RH is pretty high I figured I could do with 3 extra holes at the top of each short side for the ez dial.


28 November.



Wheat tub with the casing mostly colonized. Me thinks we'll see pins in a matter of a few days.



The round wheat bucket getting there.

I have a bunch of tubs coming from different cultures that I got started from those 2 swabs I mentioned at the start of the growlog. Cultures look really good on the PF-tek pucks and so aggressive they start to blob up right upon hitting 100% colonization, which I take it to be a good sign.


5 December.



This is a first for me shit's almost embarrassing, shrooms growing on the sides and nothing on the surface yet. This culture definitely didn't like that super thick casing. Too many things I tried for the first time here and too many things that went wrong as well.

But the game continues, have some more stuff from those swabs coming along, all cased by now;




17 December.



I've got several tubs that are blobbing right now, no pins. Fortunately I have 2 millet tubs from a different swab culture that are starting to pin.



This is the side pinning bastard today. Shitty grain, shitty prep and shitty spawn, there's little more to it. I'm not tossing this culture just yet because it's putting out some cool looking monsters and want to see what it can do with better spawn.

Same culture, same spawn, being weird and blobbing a bit in a bucket:



20 December.



Two different tubs of culture #5 from swab. These might turn out nice I think.



Culture #1 blobbing all over the place.



Culture #3, which is the same one from the tub I posted earlier which put out some 3 digit stuff. Pics after harvest yesterday;




22 December.



Dem peens today. At least we know culture #5 delivers. Going to clone something from that clusterfuck when the tubs are ready.



Culture #3 (the same culture as the tub posted earlier that was side pinning). Seems to keep pulling out some phatties. I want to see if the bucket can produce some 3 digit stuff too.


24 December.



In these LC's are the last of the cultures that were still waiting their turn to be fruited out. Culture #6 looks amazing too but I just didn't have a free LC jar for it, so that one it's going to be last to be fruited.

From each jar I inoculated 5 jars of barley spawn today.

If anyone is wondering what the hell is "cloneT", here's the story:

Quote:

Josex said:
Quote:

Josex said:

On another note,

Quote:

Josex said:


Not an update but something curious. These are the glass containers with sterile water that I used for dunking the swabs before using them and now they've turned into little LC's. :cool:

Just now I was going through my old plates to see what I could toss and found  these. I think there's enough nutes there  for the spores to germinate because these containers were used as PF-tek pucks previously and they weren't washed thoroughly at all.

Life finds a way...
:Awemazing:




I dipped my inoc loop into both jars and streaked a couple agar plates.
In one of them I got mold but the other jar tested clean.



I was expecting some bacteria from the water I dunked the swabs in but was surprised I got clean growth.






Found this little round phat guy today in the test plate. Guess who's gonna be cloned today.

:hereyougo:





26 December.

Culture #5, tub 1:


The chunkiest of the lot:


Final weight from tub 1, can't complain from MS and 3 quarts of millet:


Culture #5, tub 2 which I could've let it ride a bit longer (was spawned 2 days after tub 1), but needed to harvest today:


The chunkiest of the lot:


Final weight from tub 2:



Culture #3, bucket:


Sum crazy shit:


Culture #1, decided it was time to pin after all those blobs:



4 January.

Time to harvest culture #1 from the bucket:


Some moar freaks, it's not even funny anymore:


Mutant family

:inbred:

I'm not even sure if these are safe to eat :lol:

Current tale of the tape:
  • Culture #5: keeper.
  • Culture #3: worth giving another try imo.
  • Culture #1: would like to keep it for weird but it's terribly slow and yields poorly.


Coming next cultures: #2, #4 and #7. Culture #6 will be last to be grown.


28 January.

Culture #7 (spawned on 1/13), colonizing the casing aggressively:


Culture #4 (spawned on 1/10), this one likes it slower:


Culture #2 (spawned on 1/13):


Also, just inoculated a bunch of jars with culture #6 to finish the growlog.



Edited by Josex (01/28/21 10:25 AM)


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Josex] * 2
    #26968161 - 10/03/20 08:03 PM (3 years, 3 months ago)

THE LONE SURVIVOR


This is a second growlog that is closely related to the one above.

On July 7th I inoculated 5 PF-tek pucks with the same PE syringe I mentioned in the OP. The spores germinated fast and I made 2 transfers from every germ plate to new pucks. I have to admit I like to take clones from these pucks because they usually pin heavily. Even APE pins very well on these without a casing, some pics from a couple years ago:




The problem is that these PE pucks didn't want to pin no matter what. I wasn't too surprised by this, though. Too bad because I was hoping to snatch some easy clones.

40 days in and the pucks didn't even have some sad knots to show so I took the bait, dunked the pucks a few minutes and then applied a crappy casing layer by sprinkling some verm straight out of bag on all pucks and then misted the verm down until dark.

NOTHING... 3 weeks went by after casing and still the pucks didn't want to pin at all. I didn't want to toss them just yet so I just forgot about them.

Lo and behold, on September 12th I was preparing to toss them all when I found this in a puck:

Big phat pin. The only one out of 15 pucks. The Lone Survivor. :cool:


4 October.

So of course I cloned that bastard. Two transfers in and looking pretty good so I made an LC today. Nothing to show but some liquid in a weird jar.



12 October.



That's the 300ml LC eight days in. I could use it right now but I'd rather let it go a bit longer so there's more myc. I think it's gonna be ready in 5-6 days.


16 October.



On the left, the 300ml LC I've been posting since the beginning and on the right, a 250ml LC (.2% LME and .1% agar) that I inoculated with the same clone 2 days after I inoculated the first LC.

I wasn't planning on making a second LC so I didn't even posted it. I don't know what I was thinking putting all the eggs in one basket for the growlog so I made the second LC as a backup in case something went wrong with the first one. And thank god I did because shit did indeed happen.

I was trying to aspirate the LC when I realized the tube was completely stuck. It almost never happens but it does happen sometimes and it had to happen for the growlog, right? :grin:


Making the backup LC was not in the original plan but now that I had it I was planning on doing a side by side (regular LC versus agar LC) but now it's only going to be the agar LC, bummer. :sad:

Have to say it's the first time I'm adding agar to an LC (was inspired by Nate's TEK, although I used half the agar he says in the tek) and was greatly surprised to see how thoroughly myc colonizes the broth with the help of a bit of agar. There were not uncolonized parts of liquid to be seen like it always happens with regular LC's and myc seems to colonize the broth faster too.
The only drawback is that the agar clouds the liquid big time and I think it would be downright impossible to notice the usual visual signs of contamination in the LC.

Made some nice syringes and inoculated a bunch of jars of wheat.


I ordered the wheat online because the grain I can get locally is horrible stuff. But take a look at this online shit...


Doesn't look good at all and it's so difficult to hydrate. This is the best I could do.


I'm a little bummed with the grain situation but if this wheat doesn't work I will try another.


27 October.



This wheat is giving me shit as I expected.
These are eleven days in and they're not done yet. Shook them at inoculation (I never do that, just wanted to check something out) and then shook again when the LC recovered, and they have received today what I hope will be the last shake.

I don't trust this wheat at all so I'm going to be making another LC with this clone and will use the same wheat I'm using for the first growlog.
Regardless, I hope it works out because I inoculated all those jars you can see in the pic. Don't know what I was thinking going so big with a suspect grain.


3 November.



These are some jars after a last recovery shake. I'm not happy how they're looking. Spawned only 4 jars to CVG (1:2) and made my first ez dialed tub ala Pasty.



Depending on how the spawn behaves in the next few days I'll decide whether or not to spawn the rest of the jars.


8 November.



This is the tub 5 days in. It isn't looking bad but it could look better and it gives off a slightly bacterial smell. I'm going to case it soon.

In the meantime, I'm still holding the rest of the jars in the pic. I'm still not liking the spawn and I'm not very happy how it's performing in the tub, so I may toss these eventually and make some millet spawn instead.




11 November.



Cased it,



Since I can't seem to find plain peat without any additives, I've been using some potting soil for cactus for all my PE grows.
For all of you having the same problem, know that you can use this.
I always get such a nice coverage of the casing and very good surface conditions, myc loves it. The prep is the same as the usual 50/50 peat/verm casing, with lime and gypsum too.
This is the stuff,



Also made a bucket with this clone today, 3qts to CVG (1:2). And another bucket for the first growlog which I won't bump for such a small update.



Also noc'd some millet jars today with the LS clone.


28 November.


I know it's nearly impossible to see them in the pic but we have the first pins starting to pop up. :popcorn:


5 December.



The pinset ain't precisely breathtaking but neither was the spawn, nonetheless I have the impression these fruits are going to be cool when grown out. I still have faith in this clone.

Here's a tub I cased recently, same clone;




8 December.



They have a long way to go yet.
Yield's gonna be shitty but man aren't they purdy.

For reference, this is a 60L tub.


11 December.



First tub of the LS clon done.

Some 3 digit monsters:




17 December.



Here's the LS clone on millet spawn. I think we're going to see pins very soon.


11 January.

So the tub looked and smelled fine before casing it and after casing it too (at first) but something weird happened:



The sparse few fruits took too long to pop up and growth is obviously stunted. There's also some fuzz on the caps that looks like some kind of mold, just kept it this long out of curiosity to see what'd do.

These last days the tub was giving off an earthy smell so I was expecting trich at any moment. No trich but some kind of mold started to pop up on the surface (circled in red in the pic), so I had to toss the tub. Broke the sub apart to inspect and it looked and smelled fine. The earthy smell seemed to come from the casing only.

Same issue with the same clone, this time in a bucket:


I'm suspecting the casing. I got lazy and used some jars with casing material that had been sitting for way over a month on a shelf after pasteurization. Had the exact same issue with a tub of KSSS that also got cased with the same old casing material.

These tubs were looking great from the beginning so I was stoked to see how they'd perform from what I thought was better spawn than the spawn used for the previous grow. Guess I'll have to start over.



Edited by Josex (01/13/21 06:04 AM)


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InvisiblemushboyMDiscord
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26968167 - 10/03/20 08:08 PM (3 years, 3 months ago)

:waitwhat:

A double grow log?


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
    #26968175 - 10/03/20 08:12 PM (3 years, 3 months ago)

:hehehe:


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InvisiblemushboyMDiscord
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Re: Dem Chodes! GROWLOG. [Re: Josex] * 2
    #26968183 - 10/03/20 08:16 PM (3 years, 3 months ago)

Burying swab fibers in the pf puck is a sweet idea too.

Nicely done stuff mang


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InvisibleTormatoMFacebookDiscordReddit
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Re: Dem Chodes! GROWLOG. [Re: mushboy]
    #26968194 - 10/03/20 08:26 PM (3 years, 3 months ago)

Nice!! :rockon:

:amusedapplause:


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InvisiblePastywhyteMDiscord
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Re: Dem Chodes! GROWLOG. [Re: Tormato]
    #26968222 - 10/03/20 08:54 PM (3 years, 3 months ago)

:drunkdriver:


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InvisiblePsicomb
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26968223 - 10/03/20 08:55 PM (3 years, 3 months ago)

Great stuff Josex, I always love your threads.  Always some weird shit goin on (and I mean that in the best way possible)


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Psicomb]
    #26968277 - 10/03/20 09:34 PM (3 years, 3 months ago)

Thanks guys, let's see how this shit turns out! I'm all stoked like a fucking noob over their first cake.


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OfflineCamera93
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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26968285 - 10/03/20 09:40 PM (3 years, 3 months ago)

:bongload::camping:


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Re: Dem Chodes! GROWLOG. [Re: Camera93]
    #26968339 - 10/03/20 10:21 PM (3 years, 3 months ago)

:popcorn:


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Re: Dem Chodes! GROWLOG. [Re: JHOVA]
    #26969977 - 10/04/20 11:53 PM (3 years, 3 months ago)

:winnersurprise:


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: Tankie_J]
    #26973137 - 10/07/20 02:34 AM (3 years, 3 months ago)



Woke up today to some germination on 3 plates out of 5, nice. It's difficult to see in the pic but we have some dick myc right in the middle.


Edited by Josex (10/07/20 08:42 AM)


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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26973156 - 10/07/20 03:12 AM (3 years, 3 months ago)

:lookslucrative:


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: alaskappalachian]
    #26978348 - 10/10/20 11:03 AM (3 years, 3 months ago)



Not really an update. This is the puck where I put that fat PE pin I posted in the second growlog. Just now I found this little pin growing right off its resting father. I don't know if I should bother to clone it since it's already a clone.
The thing is it's only taken 27 days to pin and that's faster than lots of non PE varieties I've had on pucks.

On another note, temps have gone down quite a bit here and it's really slowing things down. All 5 pucks I swabbed germinated in 3 days and they have what appears to be nice clean growth but just too little to work with yet. They're taking their sweet time.


Edited by Josex (10/10/20 11:09 AM)


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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26978405 - 10/10/20 11:40 AM (3 years, 3 months ago)

Nice josex! Did you ever see that burma chose i had?


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InvisibleJosex
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Re: Dem Chodes! GROWLOG. [Re: eatyualive]
    #26978412 - 10/10/20 11:44 AM (3 years, 3 months ago)

No Eats, show me the porn don't be shy. :lol:


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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26979494 - 10/11/20 05:49 AM (3 years, 3 months ago)

:ohwow:

how do they compare potency wise to reggie cubes?


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Re: Dem Chodes! GROWLOG. [Re: LizardWizard]
    #26979505 - 10/11/20 06:05 AM (3 years, 3 months ago)

I ate 2 grams and they were strong. The people I gave them away to aren't complaining either. I've had APE and KSSS that were stronger though. Just a matter of finding a nice potent clone I think.


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Re: Dem Chodes! GROWLOG. [Re: Josex]
    #26979514 - 10/11/20 06:23 AM (3 years, 3 months ago)

:popcorn:


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