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Offlinefungi-funguy
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Liquid culture contaminated? Pictures
    #26967953 - 10/03/20 05:13 PM (3 years, 4 months ago)

Hello shroomerites! I have a few different Liquid Cultures going, it is my first time doing it but I have a ton of growth that might be mycelium on the surface of the Liquid. However idk if they could be contaminated. Chicken of the woods in particular, has what almost looks like orange yeast growing on top. I included pictures. I do plan on testing them on agar but have been swamped with work. I would a appreciate any feedback!

Thank you!


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OfflineForresterM
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Re: Liquid culture contaminated? Pictures [Re: fungi-funguy] * 1
    #26967971 - 10/03/20 05:21 PM (3 years, 4 months ago)

I can look at those and tell you they all look fine, which they do, but it means absolutely nothing as you can't see bacteria and ungerminated mold spores in liquid culture :shrug:

That's why we use agar.


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinefungi-funguy
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Re: Liquid culture contaminated? Pictures [Re: Forrester]
    #26968089 - 10/03/20 06:58 PM (3 years, 4 months ago)

You think even the one with orange looking mycelia looks okay? I guess I will just try the agar this week. Only reason I want to work with liquid culture is because I think it would colonize jars quicker since it can slide down the jar instead of agar only sitting on top.


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OfflineHarry Manbach
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Re: Liquid culture contaminated? Pictures [Re: fungi-funguy]
    #26968225 - 10/03/20 08:56 PM (3 years, 4 months ago)

It's hard for me to see what you're calling orange,but as Forrester said agar, it saves no time to have a perfectly good looking culture and put it to grains, untested, only later to find slimy or yeasty jars!

Ps. I have had pink oyster lc look off color, much darker/thicker, almost a tan/light brown? and turned out fine on grain/agar :justdontknow:  I have no experience with chicken of the woods, but I wonder if the mycelium colors with age like pinks..Or even reishi yellowing?


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OfflineForresterM
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Re: Liquid culture contaminated? Pictures [Re: fungi-funguy]
    #26968521 - 10/04/20 04:04 AM (3 years, 4 months ago)

It's not uncommon for mushroom mycelium to turn the color of the mushroom.  Like Harry Manbach (is that a Tool reference? lol) said, pinks will turn pink and I wouldn't be surprised if chickens was a bit yellow/orange.

Quote:

fungi-funguy said:
Only reason I want to work with liquid culture is because I think it would colonize jars quicker since it can slide down the jar instead of agar only sitting on top.




That's the reason I like to use it as well, it will be faster.  Just gotta test on agar first to make sure it's clean or you end up losing more time waiting for contams to show up in grain jars or, even worse, later on in subs.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineHarry Manbach
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Re: Liquid culture contaminated? Pictures [Re: Forrester]
    #26969065 - 10/04/20 12:51 PM (3 years, 4 months ago)

Quote:

Forrester said:
It's not uncommon for mushroom mycelium to turn the color of the mushroom.  Like Harry Manbach (is that a Tool reference? lol) said, pinks will turn pink and I wouldn't be surprised if chickens was a bit yellow/orange.

Quote:

fungi-funguy said:
Only reason I want to work with liquid culture is because I think it would colonize jars quicker since it can slide down the jar instead of agar only sitting on top.




That's the reason I like to use it as well, it will be faster.  Just gotta test on agar first to make sure it's clean or you end up losing more time waiting for contams to show up in grain jars or, even worse, later on in subs.





..it is...I was stuck on aenima when finally making an account.
  It's especially invaluable to test a couple plates before using the lc, considering how far a jar will last/make!  I run each quart jar with only 250-300ml of broth just so I can swirl it around real good later(no magnetic stirrer yet) and a single clean jar will last me months!


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