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OfflineRondo49
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G2A pooling ?
    #26967440 - 10/03/20 11:29 AM (3 years, 3 months ago)

Getting into agar and whenever I do a grain to agar transfer or rhizo transfers I get a lot of this weird bubbly secretions coming either out of the grain or whatever transfer I'm doing. This was from an peroxide agar Tek too.



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InvisibleMunchauzen
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Re: G2A pooling ? [Re: Rondo49]
    #26967460 - 10/03/20 11:43 AM (3 years, 3 months ago)

Thats bacteria. Do not put peroxide in your agar.


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OfflineCocaineBuffet
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Re: G2A pooling ? [Re: Rondo49]
    #26967475 - 10/03/20 11:53 AM (3 years, 3 months ago)

Quote:

Rondo49 said:This was from an peroxide agar Tek too.




:freshwtf:


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OfflineRondo49
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Re: G2A pooling ? [Re: Munchauzen]
    #26967479 - 10/03/20 11:57 AM (3 years, 3 months ago)

Why not peroxide? I can't seem to find the source of this bacteria. Using distilled water everything is clean 🥴


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InvisibleMunchauzen
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Re: G2A pooling ? [Re: Rondo49]
    #26967484 - 10/03/20 12:01 PM (3 years, 3 months ago)

Because we don't use selective media. We want anything and everything to germinate, including baddies. That is so we can see them, and either avoid using plates that possess them, or transfer away from them to clean it up. The bacteria can come from anywhere, including bad technique when having the plate open. Or it could have been bacterial grain. Its hard to say where it came from. All that matters is now cleaning it or avoiding plates with it.

If we use selective media, there could be ungerminated contaminates on the agar, and when you goto put the agar on grains, it will then germinate and ruin your grains. So we want to be able to visually inspect agar for totally cleanliness before putting it to grains.


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OfflineRondo49
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Re: G2A pooling ? [Re: Munchauzen]
    #26967493 - 10/03/20 12:05 PM (3 years, 3 months ago)

I get the part of it killing the spores but I don't wanna go that route. Only using it for transfers but I get what you are saying.


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