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Offlineshr00mFer
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Genetics question & possible contamination id
    #26963634 - 10/01/20 10:46 AM (3 years, 4 months ago)

Hi. Recently I bought some Ecuator spores and sucessfully grow shrooms using monotub (bulk). I had a couple of nice flushes but the potency wasnt good enough I believe. I cloned one big mushroom using agar and got some fluffy mycelium growing. But there is some light green stuff that i need to id (probably mold):



There is also some rizomorphic grow on the right (i put a piece from the stem on the left) which I dont know what it is??

Question about genetics is: if mycelium (on agar or elsewhwre) os strictlly tomatose (fluffy), is that the sign of bad genetics? I read it in the Stamets book, absence of rizomorphic mycelium is sign of bad vigor (loss of vigor). Either through cloning too much (maybe I got spores from mushroom that is previously cloned too much) or simply genetics are bad.

Probably thats why this strain is not so good in potency, therefore I ordered some additional variety of cubensis to compare.


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Offlineshr00mFer
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Re: Genetics question & possible contamination id [Re: shr00mFer]
    #26965009 - 10/01/20 11:26 PM (3 years, 4 months ago)

Anyone?


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Offlinesporecap
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Re: Genetics question & possible contamination id [Re: shr00mFer]
    #26965038 - 10/01/20 11:41 PM (3 years, 4 months ago)

The yellow/green blob in the middle is a bacteria colony, and the right white mycelium looks like mold. You should make a transfer asap to a new plate.
Rizomorphic/tomentose depends a lot on your agar recipe, and is usually not a good indicator for viability.


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OfflineForresterM
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Re: Genetics question & possible contamination id [Re: shr00mFer]
    #26965200 - 10/02/20 02:56 AM (3 years, 4 months ago)

Quote:

shr00mFer said:
Question about genetics is: if mycelium (on agar or elsewhwre) os strictlly tomatose (fluffy), is that the sign of bad genetics? I read it in the Stamets book, absence of rizomorphic mycelium is sign of bad vigor (loss of vigor). Either through cloning too much (maybe I got spores from mushroom that is previously cloned too much) or simply genetics are bad.





No, tomentose mycelium is not a sign of bad genetics.

If you start from spores, it doesn't matter if the mushroom they came from is cloned, you're starting over anyway so that's not an issue.

Potency is all genetics which vary in every spore.


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlineshr00mFer
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Re: Genetics question & possible contamination id [Re: Forrester]
    #26966231 - 10/02/20 04:18 PM (3 years, 4 months ago)

Quote:

Forrester said:
Quote:

shr00mFer said:
Question about genetics is: if mycelium (on agar or elsewhwre) os strictlly tomatose (fluffy), is that the sign of bad genetics? I read it in the Stamets book, absence of rizomorphic mycelium is sign of bad vigor (loss of vigor). Either through cloning too much (maybe I got spores from mushroom that is previously cloned too much) or simply genetics are bad.





No, tomentose mycelium is not a sign of bad genetics.

If you start from spores, it doesn't matter if the mushroom they came from is cloned, you're starting over anyway so that's not an issue.

Potency is all genetics which vary in every spore.






Well here is the part of the book where it is written:



Are you saying Stamets legend would lie about it๐Ÿ˜‰?


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InvisibleJosex
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Re: Genetics question & possible contamination id [Re: shr00mFer]
    #26966271 - 10/02/20 04:35 PM (3 years, 4 months ago)

Quote:

shr00mFer said:

Are you saying Stamets legend would lie about it๐Ÿ˜‰?




Stick around and you'll see that Stamets is not precisely loved around these parts. He talks mostly about edibles/medicinals and his info is often regarded as outdated as far as cultivating cubensis go.
Do yourself a favor and stop reading that guy, it's confusing and misleading you already.

The guys above me were both right and were giving you good advice. Also, you have bigger problems than having tomentose or rhizomorphic mycelium, namely, you have a lot of nasty on that plate you have to transfer from.

Tomentose vs rhizomorphic growth has more to do with conditions than anything else. Genetics also play a bit of a role. Tomentose growth is not an indication of bad genetics at all and it can perform and fruit as well or better than any rhizomophic culture. Tomentose mycelium can turn rhizomorphic if conditions change and viceversa.


Edited by Josex (10/02/20 04:44 PM)


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Offlineshr00mFer
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Re: Genetics question & possible contamination id [Re: Josex]
    #26966315 - 10/02/20 04:55 PM (3 years, 4 months ago)

Quote:

Josex said:
Quote:

shr00mFer said:

Are you saying Stamets legend would lie about it๐Ÿ˜‰?




Stick around and you'll see that Stamets is not precisely loved around these parts. He talks mostly about edibles/medicinals and his info is often regarded as outdated as far as cultivating cubensis go.
Do yourself a favor and stop reading that guy, it's confusing and misleading you already.

The guys above me were both right and were giving you good advice. Also, you have bigger problems than having tomentose or rhizomorphic mycelium, namely, you have a lot of nasty on that plate you have to transfer from.

Tomentose vs rhizomorphic growth has more to do with conditions than anything else. Genetics also play a bit of a role. Tomentose growth is not an indication of bad genetics at all and it can perform and fruit as well or better than any rhizomophic culture. Tomentose mycelium can turn rhizomorphic if conditions change and viceversa.





I didnt want to sound disrescpectfull. Didnt know people here dont like Stamets. I am having doubt about growing from that clone. Potency is not good. I ordered some new strains.


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InvisibleJosex
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Re: Genetics question & possible contamination id [Re: shr00mFer]
    #26966335 - 10/02/20 05:02 PM (3 years, 4 months ago)

If you made a print from that grow you could try your luck again starting from those spores instead of ordering new spores online. Starting from spores is a bit of a reset button, so even if those spores came from a tub of bunk shrooms the potential for good potent genetics is still there.


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Offlineshr00mFer
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Re: Genetics question & possible contamination id [Re: Josex]
    #26967457 - 10/03/20 11:42 AM (3 years, 4 months ago)

Quote:

Josex said:
If you made a print from that grow you could try your luck again starting from those spores instead of ordering new spores online. Starting from spores is a bit of a reset button, so even if those spores came from a tub of bunk shrooms the potential for good potent genetics is still there.




I have some spores lwft from original order. And I also made print from the grow (using that spores). And I cloned one big mushroom. Probably using spores is a better option, as ypu said.


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