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Offlinenektar61S
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Spores INSIDE stipe at 100X?
    #26965987 - 10/02/20 01:56 PM (3 years, 3 months ago)

Took these cell phone pix of 100 x under microsocope.

Is sliver of stipe from mature B+ cube. Had removed cap for print. Walked into other room, rinsed off with hot water. Put in lunch box, took to work and used work microscope.

Split stipe (stalk) at work, pulled off a little slice, squished between two clean slides.

Looks like spores inside stipe?

Could be spore transfer from cap, but I washed hands between, and moved to different location.

I guess I'm wondering this: does anyone know if, during the life cycle of a shroom (or specifucally a cube), do the spores go through the stipe? Pretty sure they're made in the cap. Do they get re-absored back into the ground for food or re-planting in the ground?

Is pic, then close up of pic:


Close up of same pic:


=-=-

I took the above photos. I did not take the below photo, is from Wikipedia. Is cube spores at 1000x. I can't do 1000x at work, requires oil immersion, and I would get caught if I did that there.

Just including to show that what I see in mine look the right way and size to be spores:



-=-=

post Moved from Advanced Mycology, got zero replies. Also haven't had luck posting on microscopy board, so figured someone here might know.)


Edited by nektar61 (10/02/20 02:51 PM)


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Re: Spores INSIDE stipe at 100X? [Re: nektar61]
    #26966093 - 10/02/20 03:18 PM (3 years, 3 months ago)

They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol


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Offlinenektar61S
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Re: Spores INSIDE stipe at 100X? [Re: bodhisatta]
    #26966130 - 10/02/20 03:34 PM (3 years, 3 months ago)

Quote:

bodhisatta said:
They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol




OK. Thank you. Sounds right.

I thought oil immersion is getting oil on the objective? Sounds like a mess I couldn't clean up quick.

I work night shift in a trucking warehouse, we have a scope for QC.

I'm largely there alone most nights, but my supervisor has been known to drop in. I'd have about 45 seconds of knowing he's coming in to clean up.


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Re: Spores INSIDE stipe at 100X? [Re: bodhisatta]
    #26966225 - 10/02/20 04:14 PM (3 years, 3 months ago)

Quote:

bodhisatta said:
They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol




The oil goes between the coverslip and the objective, touching both.

https://en.wikipedia.org/wiki/Oil_immersion#/media/File:Immersion_microscopy.jpg

Do you do it? Do you do it between the slide and cover slip? that's not oil immersion, as far as I know.

Doesn't it require special objectives? That's what I'm reading.

I know you have a huge font of knowledge on shrooms that I don't. And I think you are probalby right that the spores were not inside the stipe.

But I think you may be confused about oil immersion. I'm no expert on microscopes, total hobbyist, but it sounds like you're telling me to do something wrong that would wreck the scope.

The one at work only goes up to 400 x anyway. I just read that you can ruin a non-immersion objective by putting oil on it.

They're not sealed and oil gets inside.

That's how I would get caught. lol.


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Re: Spores INSIDE stipe at 100X? [Re: nektar61] * 1
    #26966356 - 10/02/20 05:11 PM (3 years, 3 months ago)

For oil immersion you would need a scope with an oil immersion objective lens. 
If you have that, it only takes a wipe with lens paper to clean up.

If you're worried about being caught playing with the scope, you could make some slides of pond water, moss, and random wild mushrooms.
Probably be less of a big deal if he walks in and you've got tardigrades to show him instead of just illegal drug spores.

Morel ascospores:


Vorticella


Some cubes to keep this all relevant:
Cap and upper gill


4 spored basidia


Edited by foragedfungus (10/02/20 05:30 PM)


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Offlinenektar61S
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Re: Spores INSIDE stipe at 100X? [Re: foragedfungus]
    #26966502 - 10/02/20 06:57 PM (3 years, 3 months ago)

Quote:

bodhisatta said:
They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol



Quote:

foragedfungus said:
For oil immersion you would need a scope with an oil immersion objective lens. 
If you have that, it only takes a wipe with lens paper to clean up.

If you're worried about being caught playing with the scope, you could make some slides of pond water, moss, and random wild mushrooms.
Probably be less of a big deal if he walks in and you've got tardigrades to show him instead of just illegal drug spores.

Morel ascospores:


Vorticella


Some cubes to keep this all relevant:
Cap and upper gill


4 spored basidia




Quote:

bodhisatta said:
They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol





Quote:

foragedfungus said:
For oil immersion you would need a scope with an oil immersion objective lens. 
If you have that, it only takes a wipe with lens paper to clean up.

If you're worried about being caught playing with the scope, you could make some slides of pond water, moss, and random wild mushrooms.
Probably be less of a big deal if he walks in and you've got tardigrades to show him instead of just illegal drug spores.

Morel ascospores:


Vorticella


Some cubes to keep this all relevant:
Cap and upper gill


4 spored basidia





Thank you, foragedfungus.

Love those images / video.

Did you use a DLSR? What adapter? I'm just using an iPhone held up to the scope.

Great idea on having other samples on the table. I didn't think of that. I didn't even think of looking at them. Sounds fun.

Did you use oil for those? Are the 1000x?

Scope I have access to only goes to 400x and doesn't have an oil immersion objective.

Is what I'm showing in / on the stipe actually spores?

If so, do you agree with bod's assessment that the spores got there accidentally from me removing the cap and splitting the stipe? Or is there a way that could happen organically within the stipe?

I did wash hands in between, but I know these fuckers are tiny.

It also makes me think how anyone growing or handling fresh shrooms is probably covered with spores all the time. lol.

We are carriers.


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Re: Spores INSIDE stipe at 100X? [Re: nektar61]
    #26966539 - 10/02/20 07:18 PM (3 years, 3 months ago)

Quote:

nektar61 said:
Quote:

bodhisatta said:
They got there when you made the slide.
Also oil immersion is literally putting a drop of slide oil on the coverslip :shrug: get caught doing what lol




The oil goes between the coverslip and the objective, touching both.

https://en.wikipedia.org/wiki/Oil_immersion#/media/File:Immersion_microscopy.jpg

Do you do it? Do you do it between the slide and cover slip? that's not oil immersion, as far as I know.

Doesn't it require special objectives? That's what I'm reading.

I know you have a huge font of knowledge on shrooms that I don't. And I think you are probalby right that the spores were not inside the stipe.

But I think you may be confused about oil immersion. I'm no expert on microscopes, total hobbyist, but it sounds like you're telling me to do something wrong that would wreck the scope.

The one at work only goes up to 400 x anyway. I just read that you can ruin a non-immersion objective by putting oil on it.

They're not sealed and oil gets inside.

That's how I would get caught. lol.



On the coverslips. Would be between the objective and the top of the slip.
On the slide would be between the coverslips and slides


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Re: Spores INSIDE stipe at 100X? [Re: nektar61]
    #26966583 - 10/02/20 07:42 PM (3 years, 3 months ago)

Yes, those are spores in the OP. And I'd agree, they got there at some point during handling.

The morel and cubes were 1000x(which uses immersion oil) 
The vorticella I found in some algae in a puddle. That video was 400x, it's cropped alot to make the gif small enough to upload.

I have a trinocular microscope, two eyepieces and a tube for a camera. It came with a  5 megapixel camera. Shooting through the eyepiece with my phone takes a clearer picture, but it's a lot tougher to do. And it is nice to have the images displayed on my laptop in real time.

I've been watching this youtube series about microorganisms and microscopy. https://www.youtube.com/c/microcosmos


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Offlinenektar61S
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Re: Spores INSIDE stipe at 100X? [Re: foragedfungus]
    #26966621 - 10/02/20 08:18 PM (3 years, 3 months ago)

Quote:

foragedfungus said:
Yes, those are spores in the OP. And I'd agree, they got there at some point during handling.

The morel and cubes were 1000x(which uses immersion oil) 
The vorticella I found in some algae in a puddle. That video was 400x, it's cropped alot to make the gif small enough to upload.

I have a trinocular microscope, two eyepieces and a tube for a camera. It came with a  5 megapixel camera. Shooting through the eyepiece with my phone takes a clearer picture, but it's a lot tougher to do. And it is nice to have the images displayed on my laptop in real time.

I've been watching this youtube series about microorganisms and microscopy. https://www.youtube.com/c/microcosmos





Kickass. All of it, and thanks for the youtube tip! That's amazing stuff. I saw that before, but hadn't been back since he upgraded to the 10,000 USD price DIC. WOW!

I was just shocked to even see spores at 100x, and by accident too.

I'd tried at 400x and didn't see any. But that was a pretty sporicious cap that my pic's stipe was from.


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Re: Spores INSIDE stipe at 100X? [Re: bodhisatta]
    #26966624 - 10/02/20 08:21 PM (3 years, 3 months ago)

Quote:

bodhisatta said:

....On the coverslips. Would be between the objective and the top of the slip....




Yes. We don't have an oil immersion objective where I work. Adding oil with this setup would probably ruin the objective, which would absolutely get noticed by the PTB.

As foragedfungus said, you need an oil-specific objective for that.

Ones that are not like that are not sealed, oil leaks in and wrecks them.


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