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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,588
Loc: Up Over
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Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 3
#26964707 - 10/01/20 08:15 PM (3 years, 4 months ago) |
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Here is an overdue log of a rare find that was sent to me by Doc9151 over a year ago of the first Psilocybe tampanensis to be discovered in Florida since Steven Pollock's single specimen was discovered in Tampa, Florida back in the late 1970's. Doc's finds were also the first recorded wild finds with actual sclerotia attached to a few of the specimens. It was logged on mushroom observer with DNA confirmed by Alan Rockefeller here - https://mushroomobserver.org/317458?q=12BpO
Here are some of Doc's photos of some of the finds -


For an interesting insight into Steven Pollock's life, death and for the story of his only find of the sclerotia producing Psilocybe he named 'tampanensis' in Florida in 1977, I highly recommend taking the time to read through this amazing article by Hamilton Morris - https://harpers.org/archive/2013/07/blood-spore/
The link to Doc1951's original finds and subsequent follow up threads can be found here - https://www.shroomery.org/forums/showflat.php/Number/25225634/fpart/1/vc/1, here - https://www.shroomery.org/forums/showflat.php/Number/25244269 and here - https://www.shroomery.org/forums/showflat.php/Number/25406226
A huge thank you to Doc1951 for the honour of working with these rare finds!
              
I received the spore print of two specimens, labelled -
#1= Psilocybe tampanensis, location perdido river usa, #2= Psilocybe tampanensis location Beulah fl usa, this is (from) the specimens Alan did the DNA analysis on.
I got around to plating up several dishes from each specimen on the 14th November 2019 -


Noticed the first signs of growth on the 19th of November -

And these were some photos I uploaded of the plates on the 3rd of December -

Transfers were taken to fresh plates of some of the fastest looking growth from each specimen -

I used a small transfer from a dark patch of agar from specimen #2 to start a small jar of sorghum. The assumption was that this may have been the start of a sclerotia and I was trying to isolate some sclerotia producing genetics. One week later the jar had colonised and growth looked very clean so was transferred to a large spawn bag of sorghum on the 11th December 2019 -

I then chose multiple clean sectors away from the inoculation sites from each multispore plate of specimen #1 to a grain jar, and repeated for specimen #2 - to capture as much genetic diversity as possible from each specimen to be able to hunt for the best looking sclerotia from each for cloning. I made 3 jars in total... two from specimen #1, and one from specimen #2 -

Im aware of the risk of working with T1's of wild plates, but i had clean transfers on fresh plates as back ups and am just using these jars to look for the best truffles for cloning. For that reason using lots of sectors and capturing a larger sample range of MS genetics was beneficial for a range of sclerotia, and all growth looked clean. I also isolated away from the inoculation sites to minimise any piggy back contaminations from the point of inoculation, but all T1 jars colonised fine.

The large spawn bag colonised in 3 days -

I noticed the first sclerotia forming in one of the jars after about 2 weeks -

After this I left the jars and spawn bag to do their thing in the back of a cupboard and forgot about them.
              
This is how things were progressing as of the 17 February 2020 -

And the bag -

Life got very busy and they were left to mature over winter and do their thing.
              
The extra time seemed to help as they really plumped up and developed a lot over the course of winter and here is how the bag is looking now -

The colonised grain jars are looking very mature and I am hoping to crack them open very soon to find the best sclerotia for cloning. I plan to grow out the best looking sclerotia from each specimen and then try and fruit each of those clones and take prints. This is how the grain jars are looking now -
Specimen #1 -

Specimen #2 -

Another jar of specimen #1 -

I'll also try and fruit what is leftover after harvesting the truffles from each jar with the aim of getting some early fresh prints of each specimen for preservation.
I'll update with pictures as soon as I get time to harvest and take clones of the best looking sclerotia and with a bio-assay result. 
Thanks again to Doc9151 for the chance to work with these amazing and rare finds! 
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 12 minutes, 16 seconds
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: _OttO_]
#26964719 - 10/01/20 08:22 PM (3 years, 4 months ago) |
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Wow so awesome. Thank you for sharing. They look amazing ๐
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: seand04]
#26964731 - 10/01/20 08:35 PM (3 years, 4 months ago) |
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-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Tormato]
#26964740 - 10/01/20 08:42 PM (3 years, 4 months ago) |
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Nice! I still have mine! I just made a bunch of agar so now that you've posted this I'll get to work on growing mine out
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MrTheseThings


Registered: 06/21/20
Posts: 51
Last seen: 3 years, 3 months
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26964749 - 10/01/20 08:52 PM (3 years, 4 months ago) |
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Wow! I can't believe more were found ! History in the making !
-------------------- These things infected me... and now I have to grow them.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: MrTheseThings]
#26965429 - 10/02/20 08:07 AM (3 years, 4 months ago) |
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So amazing
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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nogoodnamesleft
Stranger than average


Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 9 days, 18 hours
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: filthyknees]
#26965537 - 10/02/20 09:20 AM (3 years, 4 months ago) |
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In for the historical relevance
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: nogoodnamesleft]
#26965545 - 10/02/20 09:26 AM (3 years, 4 months ago) |
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Otto! ๐ค
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Psicomb



Registered: 01/13/18
Posts: 4,662
Loc: the womb
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: _OttO_]
#26965547 - 10/02/20 09:29 AM (3 years, 4 months ago) |
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Oh wow this is really friggin cool...!
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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Psicomb]
#26966434 - 10/02/20 06:03 PM (3 years, 4 months ago) |
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I'm embarrassed to say I haven't even opened my envelopes since they arrived in the mail. I'm such an asshole. That ends tonight!
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26966452 - 10/02/20 06:15 PM (3 years, 4 months ago) |
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Yeah shame on you dude.
J/k, I'm here for the popcorn.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 5 hours, 47 minutes
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Josex]
#26966456 - 10/02/20 06:21 PM (3 years, 4 months ago) |
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Asura]
#26966663 - 10/02/20 09:15 PM (3 years, 4 months ago) |
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oh ya I forgot I have two prints. is there another TC that wants one? only TCs per Doc's request.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 5 hours, 47 minutes
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26966667 - 10/02/20 09:26 PM (3 years, 4 months ago) |
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Well this is weird. I have a print from this same time frame but mine is dated 9/27/18.
I can't for the life of me remember how I got it. I'll give it a go.
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26967039 - 10/03/20 06:51 AM (3 years, 4 months ago) |
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Quote:
Munchauzen said: oh ya I forgot I have two prints. is there another TC that wants one? only TCs per Doc's request.

Right Here!!!
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Munchauzen


Registered: 06/22/11
Posts: 14,343
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Tormato]
#26967047 - 10/03/20 06:56 AM (3 years, 4 months ago) |
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sent
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Tormato  
The Goddess Kali Meh ๐




Registered: 07/01/17
Posts: 6,067
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26967071 - 10/03/20 07:15 AM (3 years, 4 months ago) |
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Quote:
Munchauzen said:
sent
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
Last seen: 9 days, 22 hours
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Tormato]
#26967085 - 10/03/20 07:25 AM (3 years, 4 months ago) |
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I feel kinda silly knowing that a lot of mushroom names begin with the place they were sourced or someone's name, and that i never realized Tampanensis comes from Florida... seems so obvious now!
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Puduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 1,666
Last seen: 2 days, 1 hour
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Munchauzen]
#26967099 - 10/03/20 07:37 AM (3 years, 4 months ago) |
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Wow wow wow.. like mentioned before. History in the making 
Cannot wait to see updates and eventually get my hands on some spores later Look at my avatar.. tampanensis loves me and I them 
Seriously tho.. sclerotia producers don't get enough love. It's so cool that we can get truffles and fruits in the same grow and the mycilium looks beautiful.. could save a grain jar for months until you feel ready to fruit 
Will be great to see the community spread these spores around the globe. Love this shit!
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Trade List LAGM2021
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Grimsweeper
don't fear the sweeper


Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 25 days, 7 hours
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Re: Doc9151's first Florida tampanensis find since Steven Pollock's in 1977 [Re: Puduwoke]
#26967146 - 10/03/20 08:27 AM (3 years, 4 months ago) |
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-------------------- When you clean a vacuum cleaner you are a vacuum cleaner Build yourself a Flow Hood in these 99 simple steps
 
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