Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Offlinemboehm
 User Gallery

Registered: 01/20/14
Posts: 95
Last seen: 1 year, 14 days
master slant fruited, best spot to transfer?
    #26965470 - 10/02/20 08:39 AM (3 years, 3 months ago)

I've had this flammulina velutipes culture for a few years on slants in storage at 1-2C. I had stepped away from the hobby for a while so the last master had gone ~18 months or so in storage by the time I recently pulled it out to try to save it. I have a few other gourmet cultures of the same age. I've gotten live tissue going from all of them and though I am skeptical of their vitality I figured I'd try to rescue them if possible.

This one fruited while in storage so I'm left with the question of where the best spot to transfer would be in this case. Would you still try to pull from the leading edge at the top of the slant if possible? Or would you "clone" as I've experimented with below (lower portion of a stipe covered in pins)?

Related: If this culture is likely an isolate, would there even be a genetic difference between mycelial and fruitbody tissues?



Edited by mboehm (10/02/20 08:46 AM)


Extras: Filter Print Post Top
OfflineForresterM
aspiring sociopath
Male User Gallery

Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA Flag
Last seen: 24 days, 19 hours
Trusted Cultivator
Re: master slant fruited, best spot to transfer? [Re: mboehm]
    #26966034 - 10/02/20 02:33 PM (3 years, 3 months ago)

Quote:

mboehm said:
Related: If this culture is likely an isolate, would there even be a genetic difference between mycelial and fruitbody tissues?





I don't believe so.  What you do need to worry about is if you get a spore in there, then that would be a different culture entirely.  So maybe take from a part of the agar that couldn't have been hit with a spore, in case there were any?  Although the culture SHOULD outrun the spore germinating, it's gonna be hard to tell for sure.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Top
Offlinemboehm
 User Gallery

Registered: 01/20/14
Posts: 95
Last seen: 1 year, 14 days
Re: master slant fruited, best spot to transfer? [Re: Forrester]
    #26966088 - 10/02/20 03:18 PM (3 years, 3 months ago)

That's an interesting point I hadn't considered. I'm not sure but I don't think they sporulated. You can see the caps in the first two pictures. They are only 2-3mm probably didn't reach maturity? Spores are white so I dunno.

Assuming the genetics are the same, I'm left wondering if one or the other is closer to senescence or at least likely to be more vigorous. I might try to transfer some of the mycelial mat to see how it compares to the first transfer I made.


--------------------
"If it smells like miso and tastes like miso,
                          ...it is probably not the best miso."


Edited by mboehm (10/02/20 04:00 PM)


Extras: Filter Print Post Top
OfflineForresterM
aspiring sociopath
Male User Gallery

Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA Flag
Last seen: 24 days, 19 hours
Trusted Cultivator
Re: master slant fruited, best spot to transfer? [Re: mboehm]
    #26966186 - 10/02/20 03:59 PM (3 years, 3 months ago)

I don't think there's gonna be much difference but try both and see what you can see!


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Early Primordia forming on agar slant (pic) ragadinks 1,683 4 11/18/03 03:22 AM
by ragadinks
* Tips or tricks for agar slants? Basidiocarp 3,456 9 01/27/04 11:50 PM
by Basidiocarp
* Pleurotus O. Fruiting felonious 2,743 17 12/30/09 06:02 AM
by Cordyceps
* Reishi fruiting from corn
( 1 2 all )
MycoFactory 7,461 22 01/26/05 09:06 PM
by chocbruce
* SHIITAKE GRAIN MASTER BAG PURCHASED FROM FUNGIPERFECTI.COM MickeyMsi 6,148 15 12/02/03 10:11 AM
by psilocyber
* Transferring from Psilos to Edibles Lindt 1,372 3 06/27/04 12:54 PM
by YidakiMan
* Wild Pleurotus Ostreatus Fruiting (NEW PICS, LESS ANTLERS) YidakiMan 4,789 18 12/26/04 05:33 PM
by lonestar2004
* Species that will fruit off of grains? Baby_Hitler 3,252 9 05/02/03 10:32 PM
by Workman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
109 topic views. 0 members, 9 guests and 0 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 14 queries.