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OfflineMycoTricho
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Agar inoculation question
    #26965007 - 10/01/20 11:26 PM (3 years, 3 months ago)

I will be making up my first agar plates tomorrow night, per Pasty's Easy Agar TEK. I would like to do all of my work at night after my family goes to bed, so that I can make sure all airflow stops in the house. So here's my question, is there any reason I can't just leave the plates in the PC through the day on Saturday and then move them straight to my SAB that night? There shouldn't be any detriment to them just sitting in there for a day, right?


--------------------
"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             


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Offlinelookintolearn
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Re: Agar inoculation question [Re: MycoTricho] * 1
    #26965100 - 10/02/20 12:26 AM (3 years, 3 months ago)

I've done that before it won't kill them or anything although I can say the condensation will probably be a lot more as that's what happened to me. However you can flip the plates over and let them sit awhile then just open them upside down and dump the pooling water out before you inoculate them.


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Offlineverum subsequentis
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Re: Agar inoculation question [Re: lookintolearn]
    #26965110 - 10/02/20 12:36 AM (3 years, 3 months ago)

I can't speak to the condensation because i fucking hate those things and don't use them but, otherwise everything will be fine.


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Offlinelookintolearn
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965117 - 10/02/20 12:42 AM (3 years, 3 months ago)

If you are going to use no pours pasty plates are viable but the holy grail containers are wayyyy better. However pasty plates are very useful for tiger drop and dropping the whole plate into LI bottle.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


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Offlineverum subsequentis
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Re: Agar inoculation question [Re: lookintolearn]
    #26965120 - 10/02/20 12:44 AM (3 years, 3 months ago)

i hate them both. But i agree.


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Offlinelookintolearn
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965130 - 10/02/20 12:55 AM (3 years, 3 months ago)

Eventually petri dishes will be the move however I just love the fact I can sterilize the no pours and whip them out whenever. I know i'm gonna love petri but I have not yet built up the reasoning to buy a shit ton when I have so many no pours available.

...That being said I do hate cleaning those little bastards.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
Offlineverum subsequentis
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Registered: 03/22/16
Posts: 8,732
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Re: Agar inoculation question [Re: lookintolearn]
    #26965146 - 10/02/20 01:14 AM (3 years, 3 months ago)

you might be over thinking the cleaning. All you really need to do is scoop the agar puck out and reuse.


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Offlinelookintolearn
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Registered: 06/02/20
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965148 - 10/02/20 01:17 AM (3 years, 3 months ago)

Yeah you're probably right. I always scoop the puck out and wash the dish and lids but since it's about to go right back into the PC that's probably unnecessary.. Good food for thought.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar inoculation question [Re: lookintolearn]
    #26965623 - 10/02/20 10:27 AM (3 years, 3 months ago)

I'm not probably right. I am right. I'm also sure that sounds egomaniacal but it's true.


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Offlinelookintolearn
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965809 - 10/02/20 12:09 PM (3 years, 3 months ago)

Honestly I like that attitude haha if you know you're right no reason to argue against it. Guess i'll just be puck chuckin with no cleanup after from now on :thumbup: Thanks man saves me some time. I guess I was just being overly cautious tbh.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar inoculation question [Re: lookintolearn]
    #26965846 - 10/02/20 12:31 PM (3 years, 3 months ago)

Unless something really strange has happened, it'll just come out nice and clean and the pc will take care of anything that remains. I'd only rinse them if there was something abnormal going on. Like if you accidentally dropped it in dog shit or something.


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Offlinelookintolearn
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Registered: 06/02/20
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965890 - 10/02/20 01:03 PM (3 years, 3 months ago)

Damn so you're telling me my dog shit agar culture is not gonna work!?



I knew something was off with that plate...


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: Agar inoculation question [Re: lookintolearn]
    #26965929 - 10/02/20 01:23 PM (3 years, 3 months ago)

honestly, i've always wanted to try making a plate out of shit. I think it'd work. I'd probably never get around to it but it'd be interesting.


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Offlinelookintolearn
Stranger becoming Strangest
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Registered: 06/02/20
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Re: Agar inoculation question [Re: verum subsequentis]
    #26965966 - 10/02/20 01:42 PM (3 years, 3 months ago)

Take a swab of your anus after a nice shit and see what spawns on the plate. Might just come up with something worth documenting.

I'm sure that at least someone has done it somewhere lol.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
OfflineMycoTricho
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Registered: 08/31/20
Posts: 100
Last seen: 3 years, 13 days
Re: Agar inoculation question [Re: lookintolearn]
    #26965981 - 10/02/20 01:50 PM (3 years, 3 months ago)

First of all, sorry for not responding. Posted before bed last night and just got off work.

Quote:

lookintolearn said:
Eventually petri dishes will be the move however I just love the fact I can sterilize the no pours and whip them out whenever. I know i'm gonna love petri but I have not yet built up the reasoning to buy a shit ton when I have so many no pours available.

...That being said I do hate cleaning those little bastards.




When you say "sterilize and then whip them out whenever", are you saying that you store the already made plates in the fridge or something? I know Pasty's write up said you could do that, but I wasn't sure if I should make just enough to use. I have limited space so I will be doing at most 3-4 jars of WBS to Shoebox for now. But if I can reliably make a bunch and store them for later use, I would rather do that. That way if I get one with contam, I can do a quick transfer with an already made up plate, instead of having to make more. Also, when you take them out of the fridge, do you let them get to room temp before using them?


--------------------
"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             


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Offlinelookintolearn
Stranger becoming Strangest
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Registered: 06/02/20
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Re: Agar inoculation question [Re: MycoTricho] * 1
    #26966710 - 10/02/20 10:29 PM (3 years, 3 months ago)

You can make no-pours and have them at room temp for months. I would say 2 comfortably but up to around 4 months or more if you pour them thicker. No need to store plate in the fridge unless you have a culture on there that you want to stall the growth until you are ready to use it.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI


Extras: Filter Print Post Top
OfflineMycoTricho
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Registered: 08/31/20
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Re: Agar inoculation question [Re: lookintolearn]
    #26966720 - 10/02/20 10:45 PM (3 years, 3 months ago)

Oh cool, that'll make them way easier to store. Thank you.


--------------------
"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             


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Offlineverum subsequentis
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Re: Agar inoculation question [Re: MycoTricho]
    #26966732 - 10/02/20 11:05 PM (3 years, 3 months ago)

i've had thickish poured petris last over a year at room temp


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OfflineMycoTricho
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Re: Agar inoculation question [Re: verum subsequentis]
    #26966738 - 10/02/20 11:12 PM (3 years, 3 months ago)

Quote:

verum subsequentis said:
i've had thickish poured petris last over a year at room temp




How do you tell if they've gone bad?


--------------------
"...do you think God made the world to amuse himself because he was bored? Because if so he would have to be mean." - Jack Kerouac

                             


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Agar inoculation question [Re: MycoTricho] * 1
    #26966745 - 10/02/20 11:17 PM (3 years, 3 months ago)

if they've dried up (it's obvious) or contaminated (also obvious).


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