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tiptrippy
The Mechanic



Registered: 09/09/20
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90% colonization PF tek jars pinning early?
#26964982 - 10/01/20 11:11 PM (3 years, 3 months ago) |
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Hey guys!
PF tek first timer. I inoculated these jars on 9/11. They seemed to be stalling out 13 days later so I was instructed to flip them upside down and wait another week or so. I flipped them upside down on 9/24.
Here is where I'm at today, 21 days after inoculation.
     
Two of the jars are starting to pin around the lid area. Is this a contamination problem or are they still healthy? Should I birth them now and dunk and roll or wait another week? This is my first grow like I mentioned before but 21 days seems extremely fast. HELP please!!
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Rafiikii



Registered: 11/17/10
Posts: 2,891
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26964997 - 10/01/20 11:18 PM (3 years, 3 months ago) |
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let them finish colonizing, looks fine.
-------------------- "You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."  
Edited by Rafiikii (10/01/20 11:19 PM)
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lookintolearn
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Re: 90% colonization PF tek jars pinning early? [Re: Rafiikii]
#26965134 - 10/02/20 12:58 AM (3 years, 3 months ago) |
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Pinning before 100% colonization is a sign of contamination. That being said the jars will probably fruit just fine even if you use the SGFC. I personally would take them to bulk though.
By the way next time you do BRF you should use half pint jars as they colonize quicker and have less a chance of stalling out.
You should also take some of those pins and put them to agar if you can. Great way to get some viable plates.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Mateja


Registered: 07/14/16
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Re: 90% colonization PF tek jars pinning early? [Re: lookintolearn]
#26965141 - 10/02/20 01:08 AM (3 years, 3 months ago) |
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Why on earth would you use supposedly contaminated cakes to bulk? That doesn't make any sense if the cakes are contaminated then surely fruiting them as cakes would be the best option for optimizing the chances of getting fruits. It's hard to tell at this point if the cakes are pinning because they are contaminated or because of some other reason but I'd definitely birth the cakes that are pinning and just scrape off the uncolonized parts
-------------------- Cakes inside Water Tub
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lookintolearn
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Re: 90% colonization PF tek jars pinning early? [Re: Mateja]
#26965150 - 10/02/20 01:21 AM (3 years, 3 months ago) |
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I suppose birthing them to a SGFC is viable for sure I just find putting them to bulk works wonders for getting more bang for your buck as far as BRF cakes go. But you are the cake god so who am I to say you're wrong.
If I get cakes that are about 90% colonize and start pinning I just scrape the uncolonized part like you mentioned and take to bulk and hasn't failed me yet.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
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Re: 90% colonization PF tek jars pinning early? [Re: lookintolearn]
#26965185 - 10/02/20 02:35 AM (3 years, 3 months ago) |
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Quote:
lookintolearn said: Pinning before 100% colonization is a sign of contamination. That being said the jars will probably fruit just fine even if you use the SGFC. I personally would take them to bulk though.
By the way next time you do BRF you should use half pint jars as they colonize quicker and have less a chance of stalling out.
You should also take some of those pins and put them to agar if you can. Great way to get some viable plates.
Yes I was so excited to start this new hobby I just used the first set of jars I could find. Shortly after inoculating them I purchased a couple cases of the wide mouth 8oz jars. Got another 9 jars going now.
I wanted to get a few good BRF runs under my belt before jumping into agar and bulk substrates. I am also very limited on space.
So is the general concensus I should go ahead and birth the jars that are pinning, remove the pins, dunk and roll then fruit them in the SGFC?
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tiptrippy
The Mechanic



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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26965760 - 10/02/20 11:31 AM (3 years, 3 months ago) |
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Thoughts?
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Mateja


Registered: 07/14/16
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26965769 - 10/02/20 11:40 AM (3 years, 3 months ago) |
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Yes you should always fruit cakes as cakes if you're looking to optimize yield and raise chances of success. I would personally never advise spawning cakes to bulk if they were made from bacterial spore solution (spore solution is almost always bacterial and that's why fruiting cakes as cakes works like a charm every time even tho inoculated with bacterial spore solution but spawning them to bulk is asking for reduced yield and possible contamination resulting in nothing)
I would just scrape the uncolonized parts of under tap water just rinse everything off and you don't have to be gentle with the cakes at all, make sure you scrape everything off that isn't colonized. check out my signature and the link for the Water Tub to further increase your chances for success assuming you at this point don't have a solid understanding of how to properly create and maintain optimal fruiting conditions in a SGFC. At least fruit half of the cakes in a Water Tub and half in your SGFC so you can find out what works best for you GL
-------------------- Cakes inside Water Tub
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
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Re: 90% colonization PF tek jars pinning early? [Re: Mateja]
#26965991 - 10/02/20 01:59 PM (3 years, 3 months ago) |
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Quote:
Mateah said: check out my signature and the link for the Water Tub to further increase your chances for success assuming you at this point don't have a solid understanding of how to properly create and maintain optimal fruiting conditions in a SGFC. At least fruit half of the cakes in a Water Tub and half in your SGFC so you can find out what works best for you GL
I try to take all advice I possibly can at this point. Read up on both of your HC teks and they seem much easier and require less maintenance. I have a couple tubs I have already drilled holes in for SGFC but I think I'll just lay some heavy duty plastic down and try out the Water Tub tek at least once. Thanks a bunch for your help!
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tiptrippy
The Mechanic



Registered: 09/09/20
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26974477 - 10/07/20 08:30 PM (3 years, 3 months ago) |
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UPDATE - - - -
Decided to wait a few more days to see what would happen since there seemed to be some mixed feelings on how to proceed. Also the jars were still growing and I didn't have a FC ready yet.
Is this mold? Will there be a point of no return if I continue to let these jars finish colonizing?
  
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Rafiikii



Registered: 11/17/10
Posts: 2,891
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26974612 - 10/07/20 10:18 PM (3 years, 3 months ago) |
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looks fine, fruit very soon
-------------------- "You didn’t come into this world. You came out of it, like a wave from the ocean. You are no stranger here."  
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Boodoo
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26974733 - 10/08/20 12:14 AM (3 years, 3 months ago) |
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Well the answer to your question is sort of inside the question. Why is it fruiting? Conditions suitable for fruiting have been met! This is commonly caused by not storing them in a dark place. Early pinning is not an issue per se, though you would want to avoid it to as it will be wasted (there's nothing wrong with squished shrooms)
I'm curious how it's meeting fruiting conditions. Particularly CO2 levels, do your jars have holes in them?
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lookintolearn
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Re: 90% colonization PF tek jars pinning early? [Re: Boodoo]
#26974813 - 10/08/20 02:04 AM (3 years, 3 months ago) |
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That's a misconception, mushrooms will fruit when they are ready. But also, if contamination is present they will fruit in an attempt to release spores before they get taken over by the contamination. You can have jars sitting in light that's been proven time and time again.
Pinning early is an issue. Doesn't mean they won't fruit more after they are birthed. But it does signify a problem occurring inside the jar.
That being said some of those jars are definitely ready to be let out.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Boodoo
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Re: 90% colonization PF tek jars pinning early? [Re: lookintolearn]
#26974862 - 10/08/20 02:57 AM (3 years, 3 months ago) |
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Quote:
lookintolearn said: That's a misconception, mushrooms will fruit when they are ready. But also, if contamination is present they will fruit in an attempt to release spores before they get taken over by the contamination.
That's true, but it's not a misconception it is a fact about growing mushrooms. I did say I was wondering why it is in fruiting conditions, you can have them in light while colonising so long as other fruiting conditions aren't met. I think his jars might have had too much FAE, too little heat and too much light. Certainly the thermometer and bright lights makes me think that.
It could be contam, I don't see any other fungi in there so maybe bacteria. Those shrooms look good all things considered so I think it's all good. Happy to be proven wrong!
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Mateja


Registered: 07/14/16
Posts: 7,948
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Re: 90% colonization PF tek jars pinning early? [Re: Boodoo]
#26975010 - 10/08/20 07:13 AM (3 years, 3 months ago) |
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Quote:
Boodoo said: Why is it fruiting? Conditions suitable for fruiting have been met! This is commonly caused by not storing them in a dark place.
Quote:
Early pinning is not an issue per se, though you would want to avoid it
"early pinning" isn't really a thing in this hobby and certainly isn't something growers are trying "to avoid". With cakes it sometimes happens that parts of the substrate are unable to be colonized and the colony considers the rest as 'full colonization' and thus fruits. But one doesn't have to wait for invitro pinning for this, just rinse off the uncolonized parts and fruit as normal and there are no issues
-------------------- Cakes inside Water Tub
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tiptrippy
The Mechanic



Registered: 09/09/20
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Re: 90% colonization PF tek jars pinning early? [Re: Mateja]
#26975042 - 10/08/20 07:47 AM (3 years, 3 months ago) |
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Yes this was my first set of jars ever and I would say there is too much FAE. The lids each have 4 holes in them and I used micropore tape to cover the holes, BUT... (because I'm a complete rookie) during inoculation I shoved the needle right through the tape and it created large holes and I did not cover them back up 🤣🤣😅. That being said, you can see my second set of jars in the background were done properly. I used large SFDs on them and just took the lids off in the SAB to inoculate.
I will birth the cakes this weekend at the latest.👍
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26975289 - 10/08/20 11:11 AM (3 years, 3 months ago) |
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The second set of jars in the background look really good, be sure to update on those
-------------------- Cakes inside Water Tub
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tiptrippy
The Mechanic



Registered: 09/09/20
Posts: 1,131
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Re: 90% colonization PF tek jars pinning early? [Re: Mateja]
#26975297 - 10/08/20 11:18 AM (3 years, 3 months ago) |
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Quote:
Mateah said: The second set of jars in the background look really good, be sure to update on those 
Yes they are muuuuch better. Will do for sure. Ill birth the first set this weekend to my makeshift water tub and update with some good pics and info on the second set. I also have another 15 half pint jars lined up for inoculation so ill do those as well.
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Mateja


Registered: 07/14/16
Posts: 7,948
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Re: 90% colonization PF tek jars pinning early? [Re: tiptrippy]
#26975411 - 10/08/20 12:33 PM (3 years, 3 months ago) |
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What even a "makeshift water Tub"? Lol, that sounds like some sort of container filled with a lil bit of water, which by definition would become an authentic functioning Water Tub
-------------------- Cakes inside Water Tub
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tiptrippy
The Mechanic



Registered: 09/09/20
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Re: 90% colonization PF tek jars pinning early? [Re: Mateja]
#26975419 - 10/08/20 12:38 PM (3 years, 3 months ago) |
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Its a 15 or 20 quart tote that I made into a SGFC but I realized that the SGFC needs to be a very specific size in order for it to work properly. So I lined the tote with clear plastic drop cloth to cover the holes and Ill follow your tek instructions for the rest of it. Do you think that will work ok?
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