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Amazon Shop for: ½ Pint Jars, Vermiculite

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Offlinesinsemillia
Stranger
Registered: 04/22/04
Posts: 8
Last seen: 12 years, 6 months
First time contamination
    #2696447 - 05/18/04 04:13 PM (12 years, 6 months ago)

A friend of mine prepared his jars using the pf tek for simple minds and sterilized with a pot of water to steam for 90 minutes. He then waited 2 days and injected 1 cc of eq spore solution into each jar at four spots. He sterilized with a lighter and then wiped the syringe with rubbing alcohol on a cotton ball. 3-5 days later all jars had a green contam but only at the point of injection so there was four green spots on each jar. He was wondering if the lighter had something to do with it or maybe the syringe was contamed. Did not use a glove box but thourghly sprayed the whole room with lysol.
Any input would be greatly appreciated.


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InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: First time contamination [Re: sinsemillia]
    #2696546 - 05/18/04 04:27 PM (12 years, 6 months ago)

The lighter had nothing to do with it.Most likely the syringe if the green is mostly around the innoculation holes.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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Offlinepeleg
Gypsy
Registered: 10/03/03
Posts: 535
Loc: Christ Light
Last seen: 12 years, 4 months
Re: First time contamination [Re: ButterNut]
    #2697348 - 05/18/04 06:05 PM (12 years, 6 months ago)

bad syringe, i've used lighter many times, what i've found to be better though is taking a shot glass and fill with rubbing alchol, set a flame to it and presto, you have a clean burning flame,peace Gypsy


--------------------
"Well the first days are the hardest days." When life looks like easy street there is danger at your door.....


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Offlinesinsemillia
Stranger
Registered: 04/22/04
Posts: 8
Last seen: 12 years, 6 months
Re: First time contamination [Re: ButterNut]
    #2698169 - 05/18/04 08:37 PM (12 years, 6 months ago)

thanks for the info.my friend ordered 4 syringes from spores 101 during the buy 1 get one free deal they had going so he should have recieved 8 total but when the package arrived he recieved only 4.He then contacted 101 and they said the 4 others would ship out on monday, well, that was 3 weeks ago and they never came. Well i guess will see if all 4 syringes are contamed.


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InvisibleButterNut
Squash

Registered: 04/17/04
Posts: 1,466
Loc: Throughout the world
Re: First time contamination [Re: sinsemillia]
    #2698178 - 05/18/04 08:39 PM (12 years, 6 months ago)

Contact them again and explain it,or PM him here.


--------------------
Butternut squash are softer than acorn or other types of squash, which makes them easier to cut and prepare.


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Offlinesinsemillia
Stranger
Registered: 04/22/04
Posts: 8
Last seen: 12 years, 6 months
Re: First time contamination [Re: sinsemillia]
    #2698179 - 05/18/04 08:39 PM (12 years, 6 months ago)

thanks for the info.my friend ordered 4 syringes from spores 101 during the buy 1 get one free deal they had going so he should have recieved 8 total but when the package arrived he recieved only 4.He then contacted 101 and they said the 4 others would ship out on monday, well, that was 3 weeks ago and they never came. Well i guess will see if all 4 syringes are contamed.


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OfflineRebirtha
I really like bread
Male User Gallery

Folding@home Statistics
Registered: 09/22/03
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Re: First time contamination [Re: sinsemillia]
    #2698294 - 05/18/04 09:14 PM (12 years, 6 months ago)

very interesting double post.


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Offlinesinsemillia
Stranger
Registered: 04/22/04
Posts: 8
Last seen: 12 years, 6 months
Re: First time contamination [Re: Rebirtha]
    #2700011 - 05/19/04 06:39 AM (12 years, 6 months ago)

yeah, sorry bout that, i hit reply and it said post allready exist so i hit quick reply and it went through so it must have went twice.


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Offlinehawksapprentice
Yearns to Snowboard
 User Gallery

Registered: 06/06/03
Posts: 3,193
Loc: Oregon
Last seen: 2 months, 27 days
Re: First time contamination [Re: sinsemillia]
    #2700017 - 05/19/04 07:02 AM (12 years, 6 months ago)

If your looking for a good supplier Hawkseye have never done me wrong...always good service and never poor or contamed syringes.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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OfflineGeckoKing
Not a Wookie

Registered: 08/31/01
Posts: 30
Loc: The Plains
Last seen: 12 years, 4 months
Re: First time contamination [Re: sinsemillia]
    #2723182 - 05/24/04 04:12 PM (12 years, 6 months ago)

what strains did you buy?

I also bought the deal from Spores 101 and my PESA and EQ syringes are bad. Green mold at the innoculation points withing 4 days.

Caused me about 2lbs worth of flour and equal vermiculite.


--------------------
People are Strange When You're a Stranger


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Amazon Shop for: ½ Pint Jars, Vermiculite

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