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gabbk
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Pan cyan first time! [COMPLETED]
#26962587 - 09/30/20 06:28 PM (3 years, 4 months ago) |
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Hey guys. I've made some plates of pan cyan. Here's a germination plate (there are a few snags on the plastic, sorry about that). There are 3 spots with a little area of bacteria in the middle.
I'm needing directions of which sectors are best to transfer. I've read that pan cyan is always tormentose and wispy, but what takes my attention is that whiter and brighter mycelium of the bottom. Is that healthy mycelium or contaminated with mold? Which sectors do you suggest to transfer?
And here's another pic, these two were already transfered once from another two different germination plates. Any opinion on these two, on which sectors to transfer?

Any feedback is very much appreciated!
Edited by gabbk (11/19/20 06:18 PM)
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Asura
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#26962672 - 09/30/20 07:20 PM (3 years, 4 months ago) |
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cyans is wispy...anything tomentose you should toss. In the second pic (first dish), you want to transfer from the top right from about 8-10 o'clock.
It's the fastest mover and those bits have the look you want. I would personally toss everything else.
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: Asura]
#26962708 - 09/30/20 07:35 PM (3 years, 4 months ago) |
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In going to take two good samples of that sector then! Thanks Asura!
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#26962808 - 09/30/20 08:29 PM (3 years, 4 months ago) |
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So those white and brighter bits of the first pic aren't healthy right? Do you know what could it be? Maybe a mold mixed with the myc?
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Edited by gabbk (09/30/20 08:29 PM)
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99.99
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: Asura]
#26962856 - 09/30/20 08:52 PM (3 years, 4 months ago) |
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Quote:
Asura said: cyans is wispy...anything tomentose you should toss. In the second pic (first dish), you want to transfer from the top right from about 8-10 o'clock.
It's the fastest mover and those bits have the look you want. I would personally toss everything else.
Is the area highlighted in yellow what you mean ?? I am just starting pan cyan also
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Asura
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#26962969 - 09/30/20 09:59 PM (3 years, 4 months ago) |
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Quote:
99.99 said: Is the area highlighted in yellow what you mean ?? I am just starting pan cyan also

I mean the first dish in this pic. Top right corner. It's in the best overall shape I think.
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99.99
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: Asura]
#26963090 - 09/30/20 11:18 PM (3 years, 4 months ago) |
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Thank you for clearing that up for me your help is greatly appreciated I’ve never been able to master the pan Cyan
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: 99.99]
#26966301 - 10/02/20 04:46 PM (3 years, 4 months ago) |
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Well, I made some transfers, so in the next few days I'll post some more pics showing how the selection is developing.
I've been using the search button looking for G2G vs LC specifically for Pans and couldn't find any solid info. Why you guys (Asura, Mary) use LC directly? Would it be improper/are there any disadvantages in doing a few different jars with different agars and then expanding by G2Ging them?
I do not have experience with LC, but definitely a lot with G2G. I wouldn't mind learning to G2G though.
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Asura
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#26966401 - 10/02/20 05:43 PM (3 years, 4 months ago) |
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I like LC because it works for me 
I would just make a bunch of jars and skip the g2g bit. Spawn ratios are low with pans 1:4, even 1:6. I think cultures fruit better when they are younger, so I think the g2g could end up being a bust. But hey, I've never done it so you may want to give it a go.
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A.k.a
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: Asura]
#26967040 - 10/03/20 06:52 AM (3 years, 4 months ago) |
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This is myc from Cambo clones, it’s a little thicker and brighter than I normally get from germ plates.
 
The plate on the right in the first picture is more like what I normally look for, especially the growth around the edge which is wispier.
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LAGM2020     
Edited by A.k.a (10/03/20 06:53 AM)
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99.99
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: A.k.a]
#26969352 - 10/04/20 03:52 PM (3 years, 4 months ago) |
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After reading this thread I went back and isolated what I thought was similar what do you think
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A.k.a
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: 99.99]
#26969357 - 10/04/20 03:54 PM (3 years, 4 months ago) |
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Looks good to me. Seems to have recovered and spread quickly which is perfect.
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LAGM2020     
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: A.k.a]
#27006668 - 10/27/20 04:19 PM (3 years, 3 months ago) |
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Hey guys, I'm back again! I did a few couple transfers and this is what I got. I think I catched some good growth finally:
 (Sorry for the plates with condensation. They were poured not long ago, but they all look very similar tough).
And also when doing these transfers I tried making my very first LC with an agar wedge on oat water diluted 1:4 with tap water. I suspect it's bacterial. What's your opinion on it? I might do another LC these days if it's dirty:
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A.k.a
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#27006859 - 10/27/20 06:12 PM (3 years, 3 months ago) |
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Nice! Plates look good for sure.
The lc definitely looks dirty to me but I’ve never used grainwater. How long did you cook it?
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LAGM2020     
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: A.k.a]
#27006931 - 10/27/20 06:44 PM (3 years, 3 months ago) |
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Thanks! Crossing fingers here for the grows I made 2 LCs parallely, one of Pan LC the other APEU. Both of them were sterilized 20 minutes in the same PC with the exact same recipe. The APEU one looks clean to my untrained eye; it looks a lot clearer than the Pan LC:
 I had to ask because I thought maybe Pans could behave differently being in LC. So we can conclude that the wedge had some bacteria in it. Now I can discard it with peace of mind haha
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Edited by gabbk (10/27/20 06:44 PM)
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99.99
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#27006995 - 10/27/20 07:15 PM (3 years, 3 months ago) |
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Quote:
gabbk said: Hey guys, I'm back again! I did a few couple transfers and this is what I got. I think I catched some good growth finally:
 (Sorry for the plates with condensation. They were poured not long ago, but they all look very similar tough).
And also when doing these transfers I tried making my very first LC with an agar wedge on oat water diluted 1:4 with tap water. I suspect it's bacterial. What's your opinion on it? I might do another LC these days if it's dirty:

Nice I’m right behind you I’m going to transfer my plate that looks equivalent to yours and try to get a few more before I take it to wheat
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Roundabout
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: 99.99]
#27007242 - 10/27/20 09:35 PM (3 years, 3 months ago) |
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I've only got one pan grow under my belt but it went pretty well. Those plates all look pretty good to me.
I tried various LCs and in the future I'm just going to stick to karo I think. The grain water was way too cloudy. LMEA was good for the first jar I poured it into but the subsequent ones ended up with gunk that hadn't dissolved. Karo is cheap and stays clear.
Thankfully my pan cyan culture went into the LMEA/Karo mix that stayed clear:

Super filtered store honey might work. I tried with honey from the bees I keep and it was barely filtered, there was just too much gunk in it.
Edited by Roundabout (10/27/20 09:35 PM)
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gabbk
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: Roundabout]
#27026501 - 11/07/20 10:15 AM (3 years, 3 months ago) |
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I'm back once more with some more few pics! How is this looking?

 I did all of these following JOC tek at 5cm deep (2in). I certainly know I won't achieve a top notch grow in this first try with this method lol, but I'd be super happy with just a couple of grams  As I don't have a greenhouse or any sistem with air extraction and high humidity, I thought wrapping them up with saran wrap and making a few holes for colonizing stage, and then, after casing, fruiting them in a bag with holes, just as bodhisatta's shoebox style with cubensis. Do you think it's gonna somewhat work with this specie?
I have some other questions too if it isn't too much bother. I would really appreciate if you could answer these 1) I suspect they need more FAE, is that right? How would I notice if so? 2) What's the visual cue to mist? Is it is just as cubes, when the droplets dry out? 3) Is there any visual cue also for knowing when to case and fruit? (They have 4/5 days from spawn right now, idk if I should wait or do it now)

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Edited by gabbk (11/07/20 11:03 AM)
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coversall
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: gabbk]
#27026631 - 11/07/20 11:23 AM (3 years, 3 months ago) |
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My pan agar is just coming along so I'm following your grow! Exciting stuff!
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A.k.a
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Re: Pan cyan agar first time, trouble recognizing healthy myc [Re: coversall]
#27026746 - 11/07/20 12:30 PM (3 years, 3 months ago) |
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Those look good so far.
I’d give it another day or two and case the circle ones.
You’ll definitely get pins but keeping them alive can be tricky, they need to be wet. Like you can’t possibly go too wet as long as there’s good airflow.
The set up I use for mine is similar to what you’re talking about with with the Saran Wrap only I’ve got a fogger going in. You might have trouble getting enough air and evaporation though, it’s extremely dry here so it works fine for me.
Pans take a ton of tuning initially but then once you get everything balanced they’re just like cubes.
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LAGM2020     
Edited by A.k.a (11/07/20 12:33 PM)
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