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Offlinepitriot
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MSS to Agar?
    #26962457 - 09/30/20 05:06 PM (3 years, 4 months ago)

When putting MSS to Agar is it best to use just a tiny drop and if so why? And is it better to inject multiple spots on the agar or only one ?


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OfflineBig_Dub
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Re: MSS to Agar? [Re: pitriot]
    #26962462 - 09/30/20 05:09 PM (3 years, 4 months ago)

it is best to use a tiny drop, to minimize contamination. There are millions of spores in there. Just do one drop right in the center. make new plates for transfers.


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Offlinepitriot
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Re: MSS to Agar? [Re: Big_Dub]
    #26962475 - 09/30/20 05:17 PM (3 years, 4 months ago)

Is there any tip/trick how to get get only a tiny drop out of the syringe? Usually for me it is hard to control it and have it only eject a tiny drop.


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Offlinepitriot
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Re: MSS to Agar? [Re: pitriot] * 1
    #26962488 - 09/30/20 05:22 PM (3 years, 4 months ago)

Also does it make a difference if inject spores or culture?


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InvisibleMunchauzen
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Re: MSS to Agar? [Re: pitriot] * 1
    #26962524 - 09/30/20 05:48 PM (3 years, 4 months ago)

shoot a small bit out of the syringe onto the bottom of your sab. after you break the initial seal that can form in the needle, its easier to push individual drops out


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Offlineroarkell
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Re: MSS to Agar? [Re: Munchauzen]
    #26962559 - 09/30/20 06:07 PM (3 years, 4 months ago)

Also, swabs can be very beneficial for spore germination... they absorb a lot of the excess water and you can run them over multiple plates to really spread those spores thin.


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Invisiblesh4d0ws
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Re: MSS to Agar? [Re: roarkell]
    #26962580 - 09/30/20 06:20 PM (3 years, 4 months ago)

Yeah I quite like squirting spore solution onto a sterile swab and then doing a zig-zag pattern for germ plates

You can try to just put a small drop but I would more often than not squirt too much and then it makes a mess of the dish


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Offlineroarkell
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Re: MSS to Agar? [Re: sh4d0ws]
    #26962586 - 09/30/20 06:26 PM (3 years, 4 months ago)

I never had any luck with spore syringes to agar... they all went bacterial on me before I could even get myc to grow... swabbing was a game changer for me...


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Offlineseand04
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Re: MSS to Agar? [Re: sh4d0ws]
    #26962591 - 09/30/20 06:32 PM (3 years, 4 months ago)

Quote:

sh4d0ws said:
Yeah I quite like squirting spore solution onto a sterile swab and then doing a zig-zag pattern for germ plates

You can try to just put a small drop but I would more often than not squirt too much and then it makes a mess of the dish



Thats how I do it. I also invert the syringe so that the spores go to the top of the syringe


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Offlinepitriot
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Re: MSS to Agar? [Re: roarkell]
    #26962716 - 09/30/20 07:40 PM (3 years, 4 months ago)

Quote:

roarkell said:
Also, swabs can be very beneficial for spore germination... they absorb a lot of the excess water and you can run them over multiple plates to really spread those spores thin.




Do you mean to use the syringe to wet the swab? When using a swab how would you get the equivalent of only using a tiny drop from a syringe?


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Offlineseand04
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Re: MSS to Agar? [Re: pitriot]
    #26962907 - 09/30/20 09:26 PM (3 years, 4 months ago)

Quote:

pitriot said:
Quote:

roarkell said:
Also, swabs can be very beneficial for spore germination... they absorb a lot of the excess water and you can run them over multiple plates to really spread those spores thin.




Do you mean to use the syringe to wet the swab? When using a swab how would you get the equivalent of only using a tiny drop from a syringe?



Yes remember spores are microscopic.  So a lil bit of solution with spores will soak up the excess solution so more of the spores hit the plate. I like to do a Z pattern. GL


Edited by seand04 (09/30/20 09:29 PM)


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InvisibleMtbromo
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Re: MSS to Agar? [Re: seand04]
    #26962918 - 09/30/20 09:32 PM (3 years, 4 months ago)

No pour agar jars tek. Inject iso and flamed MSS into the FAE hole inside a SAB into the middle of the plate, then re tape hole with Micro pore tape. After a few days flip jars upside down.



If you wanna be a gangsta make a injection port with gasket glue or silicone on your lid as well as a FAE hole. (A bit over kill but up to you)

Full proof newb agar coming your way :smile:


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Offlineseand04
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Re: MSS to Agar? [Re: Mtbromo]
    #26962928 - 09/30/20 09:37 PM (3 years, 4 months ago)

Quote:

Mtbromo said:
No pour agar jars tek. Inject iso and flamed MSS into the FAE hole inside a SAB into the middle of the plate, then re tape hole with Micro pore tape. After a few days flip jars upside down.



If you wanna be a gangsta make a injection port with gasket glue or silicone on your lid as well as a FAE hole. (A bit over kill but up to you)

Full proof newb agar coming your way :smile:



Dope AF, probably might help with condensation :rockon:


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InvisibleMtbromo
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Re: MSS to Agar? [Re: seand04]
    #26962967 - 09/30/20 09:59 PM (3 years, 4 months ago)

Apparently if you let the jars cool in the PC overnight it reduces condensation.

Slower temperature change over longer time I guess


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Offlineseand04
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Re: MSS to Agar? [Re: Mtbromo]
    #26962990 - 09/30/20 10:07 PM (3 years, 4 months ago)

Quote:

Mtbromo said:
Apparently if you let the jars cool in the PC overnight it reduces condensation.

Slower temperature change over longer time I guess



I do that and still get condensation.  But it only effects a small portion,  but I am gonna start pouring it out in my SAB then use a sterile wipe to clean it up.


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InvisibleMtbromo
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Re: MSS to Agar? [Re: seand04]
    #26963018 - 09/30/20 10:21 PM (3 years, 4 months ago)

Like open the jar and wipe inside?!?! :mad::ooo:

Danger!


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Offlineseand04
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Re: MSS to Agar? [Re: Mtbromo]
    #26963029 - 09/30/20 10:24 PM (3 years, 4 months ago)

I have been thinking like you said, just turn them over. Then when I open em just pour out the moisture. From the lid


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Offlineseand04
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Re: MSS to Agar? [Re: Mtbromo]
    #26963031 - 09/30/20 10:26 PM (3 years, 4 months ago)

Quote:

Mtbromo said:
Like open the jar and wipe inside?!?! :mad::ooo:

Danger!



No never. That would be plate suicide


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Offlinepitriot
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Re: MSS to Agar? [Re: seand04]
    #26963394 - 10/01/20 07:41 AM (3 years, 4 months ago)

I have done no pour agar and gotten condensation but I let then sit around (not upside down) and eventually the condensation went away. I used pp5 containers (mostly Target generic or Dollar Tree containers).


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Offlinepitriot
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Re: MSS to Agar? [Re: seand04]
    #26963396 - 10/01/20 07:43 AM (3 years, 4 months ago)

>>Yes remember spores are microscopic.  So a lil bit of solution with spores will soak up the excess solution so more of the spores hit the plate. I like to do a Z pattern. GL

Wouldn't that be equivalent to injecting a lot of MSS as oppose to just a tiny drop?


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