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InvisibleTFI
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Best way to store mason jar agar dishes/plates
    #26962213 - 09/30/20 02:47 PM (3 years, 3 months ago)

Alright everyone I have finally got my agar technique down and have  6 sets of isolates But can only use one at a time.

What’s the best way to store the jars without them drying or hurting the mycelium. It took forever to get here I don’t wanna waste it lmao.

Also how many times can I transfer the same isolate before I see negative effects. Thanks guys


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OfflineOne of Us
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Re: Best way to store mason jar agar dishes/plates [Re: TFI]
    #26962254 - 09/30/20 03:04 PM (3 years, 3 months ago)

Stick em in the fridge to slow their growth. They'll be good for at least a month or two depending on how thick you poured. Just be careful of unintentional freezing. Couple of weeks ago I got some plates out of the back of my fridge, and they were encased in ice :facepalm: Most surprisingly grew back, but I wouldn't recommend it :lol:

To answer your other question, are you sure they are true isolates? It takes dozens of transfers to get an isolate from MS. It takes much less transfers if you start with a clone however.

You should look into keeping master and slave plates to avoid expanding a culture too much. Here's a good thread verum wrote on the subject.


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Re: Best way to store mason jar agar dishes/plates [Re: One of Us]
    #26962311 - 09/30/20 03:29 PM (3 years, 3 months ago)

Yeah took a clone then let it recover then took a sector then let that grow out then took another sector after that. So far it’s very uniform like picture perfect round. With the mason jars it’s a little hard to see exactly what’s isolating and what not but I believe I have it down I get all the light I can and I can’t see where it’s truly tight and ropey vs a section that’s whispy.

As for the depth of agar I would say I have between 1/16-1/4 in depth between plates. Just need em to slow down for about a month maybe 2


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