Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Next >  [ show all ]
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: starbones]
    #26987489 - 10/15/20 06:28 PM (3 years, 3 months ago)

Yeah sorry about misnaming you, I went back earlier and changed that.

It might be time to be generally nicer now though.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 15 hours
Re: Room air purification [Re: Pastywhyte]
    #26987490 - 10/15/20 06:28 PM (3 years, 3 months ago)

Also, just to point out as I did in the other thread, the fact that there may be aerosolized mycelium in the air does not change the results of actual experiences of most people here.

Experimental data (experience)is much more valuable than theoretical ideas.

In theory, you would think aerosolized mycelium will definitely affect a grow, but that doesn't change the actual experience people like pasty and many others have had.

Also, trich wasn't specially mentioned in that paper. Not saying it doesn't aerosolize, it most likely does,  but it isn't exactly truth to say it does


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: One of Us]
    #26987497 - 10/15/20 06:31 PM (3 years, 3 months ago)

What it did mention specifically was aspergillis versicolor, the main mold I struggled with.

My point about not everyone having those same experiences is apparently worth repeating as well.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: Violet]
    #26987515 - 10/15/20 06:47 PM (3 years, 3 months ago)

Don't be sorry man/ma'am I know you weren't doing it to get my goat.

As for aersolized or not I just won't take risks with my lungs anymore. Respirator filters cost nothing and it syncs with my belief about cleanliness in a home. An ounce of prevention is worth a pound of cure. I would not like this to be twisted into me saying people should wear respirators as a rule. I just know how FUBAR my lungs are and wish back a decade and a half ago I wore proper PPE instead of dust masks when dealing with all the lovely shit that grows in the dark.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Room air purification [Re: Violet]
    #26987524 - 10/15/20 06:54 PM (3 years, 3 months ago)

Quote:

Violet said:
You’re totally right of course, which is why I’ll clarify that none of us have said we actually believe environmental mold is surely OP’s problem and not the spawn, simply contesting a questionable absolute made by others frequently on the forum.

I went and looked at what Reddit had going for mushcult precisely one time lol.


Also, I went and found the study Munchausen referenced. It’s pretty good shit so far, just from the abstract at least.

Here it is for anyone interested.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC126767/




I’m definitely interested, thanks


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 15 hours
Re: Room air purification [Re: Pastywhyte]
    #26987570 - 10/15/20 07:27 PM (3 years, 3 months ago)

Quote:

Violet said:
What it did mention specifically was aspergillis versicolor, the main mold I struggled with.

My point about not everyone having those same experiences is apparently worth repeating as well.




I understand people have other experiences. I personally have no experience with aspergillis, so I can't comment on that.

I do have plenty of experience with trich and all of my posts in both these threads were with regards to my experience and knowledge of trich. Trich is the topic of both threads and definitely the most common contam experienced here.

I never new aspergillis could be so pervasive. Asp. outbreaks are rarely talked about here, so thank you for opening my mind to that fact.  I always see crazy colors in new peoples first grows, but I honestly had no idea that experienced growers had major problems with mold other than trich. I feel it is important to emphasize that I consider you an experienced grower, not a noob. I mean no disrespect, I honestly just never heard of someone with experience really struggling with something other than trich (probably because its so relatively rare). Please excuse my ignorance


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: One of Us]
    #26987597 - 10/15/20 08:06 PM (3 years, 3 months ago)

I'd say there's a major overrepresentation of "trichoderma" when it comes to people and their experiences with contamination.
Plenty of molds out their common to the household that people could believe was trich when it's really not because a second opinion wasn't chased after and in their minds mold=trich.

Trichoderma is the Kleenex of the mushroom cultivation world now.

I keep a cabinet full of horrors and here are some of the latest to join the family. Keep in mind I juggle 244-380 jars at a time so it's not common I get these but when I do they fascinate me.  Only reason I keep them around.



I got some weird fuckers too. Jars where the oats turned jet black, agar plates with red ooze. Couldn't begin to tell you what any of them are.


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: starbones]
    #26987599 - 10/15/20 08:09 PM (3 years, 3 months ago)

It's totally cool of course One Of Us :thumbup:

I misremembered a bit. I definitely dealt with one variety or another of Aspergillus now and then, but I'm thinking now that my more consistent problem was actually Penicillium. I'm glad it has been long enough since I had the consistent issues for me to forget.

It was also mentioned in the study too.


And I agree with starbones that "trich" sometimes ends up said as a catch-all here for mold contam.


I went and pulled this since you mentioned not being familiar with Asp. as a contaminant mold in mush cult
Quote:

Kizzle said:
Aspergillus
Odors: Musty, Oily
Sources: Soil, Wood, Dust, Air

Also very common indoor air, like Penicillium, Aspergillus species produce tiny spores which can travel relatively large distances before settling. Many species which are common contaminants of mushroom substrates. Mycelium is usually light grey with linear threadlike growth and may be mistaken for mushroom mycelium. Some colonies may appear ringlike with denser mycelium near the edges. Aspergillus produces it's spores on large sporophores which change color as they mature. Color and size of sporophores varies by species and substrate with yellow, black, green, blue and grey all being common, making it easy to mistake with other mold species. Aspergillus is a common contaminant of grain spawn and can be recognized by it's very grainy appearance as spore production begins.



Known contaminant species include:
A. flavus, a yellow to green mold, can produce large amounts of toxins
A. niger, dark brown pins on a white-yellow mycelium
A. fumigatus, blue/grey to green/grey, highly pathogenic to people with suppressed immune systems
A. versicolor, produces a wide a variety of colors




--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: Violet]
    #26987607 - 10/15/20 08:12 PM (3 years, 3 months ago)

Oh I recognize that bright green shit right there, oh boy do I ever because that's the rotten shit that was growing on 1/2-3/4 of the 40+ tubs I lost during my hiatus from my basement. Greys, browns, every flavour of the rainbow but that neon green is burned into my eyebones.


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 15 hours
Re: Room air purification [Re: starbones]
    #26987616 - 10/15/20 08:21 PM (3 years, 3 months ago)

Yea, penicilium is much more common. I see it all the time on my plates. I don't see it in my tubs tho.

Haven't experienced aspergillis tho. Maybe on my petris, but if I did I tossed the plate before it sporulated


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Room air purification [Re: starbones]
    #26987779 - 10/15/20 11:34 PM (3 years, 3 months ago)

Why not use bags if you’re juggling 300 quart jars?


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: Pastywhyte]
    #26987795 - 10/16/20 12:01 AM (3 years, 3 months ago)

I have 500 of the XL 0.5 micron unicorn bags but despite my best efforts thus far I have not been able to have consistent success. Same LC I use in my jars, tests clean on agar. The majority of my bags go to shit, some don't but even those I don't trust and I bag fruit those.



At first it was mentioned to me that I could be getting a bad seal due to a sealer having trouble going through the folded gussets so I moved over to a larger sealer so I could expand the gussets. Kept my oats dry. These bags sat un-inoculated for weeks and didn't seem to have any issues. A week into being knocked up and well.. always the same. Haven't figured out what I'm doing wrong and didn't want to bother anyone here with it. I keep plugging away at them though.

But yeah, same LC which tests clean. Dryer than normal oats, rolled to get the air out prior to pc. Sealed, PC vented for 10 minutes then bags are placed accordion folded with jar ring spacers to make sure as much steam gets on them as possible. Filter patches always come out dry. I tried moving from 3 hours to 4 hours per run but I'm sure the failure mode here is something I'm doing wrong during inoculation/colonizing. Each bag gets a fresh 18g needle, poked above the grains and given a 10ml spritz, all done infront of the flowhood I do plates and agar to LC's.

I'll figure out where I'm going wrong eventually, I doubt it's the 0.5 micron patch as many Shroomery users claim to have no problems with those. The odd bag or two I'll find the plastic went super thin during PCing in a spot or two and who knows maybe they're drawing in air just enough through a pinhole.

All that aside jars are really pretty.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Room air purification [Re: starbones]
    #26987800 - 10/16/20 12:12 AM (3 years, 3 months ago)

If you’re using a flowhood poking a needle through the bag is ham fisted IMO. I wouldn’t even use a needle for LC with bags, just pour a bit in like G2G if I had use of a flowhood. I used to make up a bunch of small LCs and let them get thick. Then I would just pour it right into the bags, seal (I use zip ties to seal) and bam! Lots of the time the poured LC recovers far faster than when it’s shredded by the needle.

Course I also make LC very differently than most people and use more complex nute sources. It gives an edge to the pour method.



Extras: Filter Print Post Top
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: Pastywhyte]
    #26987806 - 10/16/20 12:18 AM (3 years, 3 months ago)

^^^^ I was about to say nearly the same thing.
If you have sterile airflow, don't seal your bags before sterilizing them, just be careful with the tops until you close them! Put reusable tyvek suit pieces in the tops of the bags from nearly sub level to out the top so that no vacuums are created, let them cool with them in, and pull the pieces out immediately before inoculation.  Even when I might use a needle to draw up LC I still just squirt it down the bag's opening. I also don't bother using a sealer - I just zip-tie the top, usually around a fold, careful to close the top in a way that won't let spores get in first.

I couldn't say for sure that your terrible contams all come from fresh needles through fresh sterilized inoc ports pulled into sterile airflow, and I'm a bit curious about the reason for your contams too, but that's definitely something you could do regardless and it might make a difference for you.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: Pastywhyte]
    #26987815 - 10/16/20 12:26 AM (3 years, 3 months ago)

I will have to give that a try might start with G2G or squirt it in a hole. I seal my bags before PC so I assume there's no problems to cut them open in front of the flowhood with a sterile blade then just make a neck and zip-tie the shit out of it like you did?

Can they be zip-tied before PCing? My brain is telling me that shouldn't be a problem because I use zip-ties that sit next to piss-red hot diesel exhaust manifolds but better to ask.

I'll give it a shot because more spawn more better. Thanks a bunch.

No inoculation ports on these bags, I mark where I aim to poke with a sharpie, clean it with 70% iso, throw on a fresh needle and squirt then cover the hole with tape. I've tried everything from electrical tape to tuck tape to masking tape. Mystifies me as well because in the same inoculation run I could do 6 or so bags followed up by 50 jars and won't lose a jar but will lose 5/6 bags. Electric stove not gas because I read folks had a problem with gas melting bags.

Is that a definite no-go on pre-sealing? Shroomery informations all over the map but the most current information I could find is that 0.5 micron patches were fine for pre-sealing. Even if I could get away with a zip tie I'd feel oodles better if I knew it was like one of my jars coming out of the PC, sterile (as we can get them) until I fuck with it. The accordion shennigans just felt like something I'd fuck up since I'm a visual learner not so handy with the word learnin'


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Room air purification [Re: starbones] * 1
    #26987816 - 10/16/20 12:29 AM (3 years, 3 months ago)

I only ever tried presealing once. Didn’t like it, felt impossible to get a decent plenum.


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: Pastywhyte]
    #26987819 - 10/16/20 12:32 AM (3 years, 3 months ago)

+1

(also had some bursts)


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Offlinestarbones
I'm an alien, I eat uranium.
I'm a teapot


Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
Re: Room air purification [Re: Violet]
    #26987833 - 10/16/20 12:51 AM (3 years, 3 months ago)

Shit on a shingle well I guess come morning I will attempt to source tyvek (sleeves?) and dig deep for a pictorial on their usage so I can comprehend their usage.

Gracias.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,809
Loc: Canada Flag
Trusted Cultivator
Re: Room air purification [Re: starbones]
    #26987868 - 10/16/20 01:32 AM (3 years, 3 months ago)

I’ve used aluminum foil instead of sleeves myself and actually prefer that to tyvek. I’m mostly just looking to prevent the bag from basically melting shut. You just need a barrier, not filtration material despite RR’s claims otherwise.


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery

Registered: 12/06/11
Posts: 4,205
Re: Room air purification [Re: Pastywhyte]
    #26987869 - 10/16/20 01:35 AM (3 years, 3 months ago)

Yeah I figured it's not a filter so much as something to prevent a de facto seal that could create a pressure difference. Never thought about aluminum though! I'll remember that, because my tyvek pieces don't look so hot these days lmao


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Next >  [ show all ]

Shop: North Spore Bulk Substrate   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* experimental air purification stinkbuttdog 562 5 04/24/07 02:18 PM
by RoachMan
* water purification? dressel11 484 2 01/04/05 02:59 PM
by KaptKid
* Grass Clippings Instead of Rice Flower cen_tex_looker 1,246 1 07/21/02 10:00 AM
by Anno
* Using spores instead of spawn for dung Land_Crab 1,226 9 04/06/05 11:34 PM
by scatmanrav
* just an airstone and a jar of water? (instead of perlite) tokey666 871 5 09/15/05 05:38 PM
by Pixie1420
* brf cakes spawned to bulk instead of birdseed shroomer68 1,664 4 12/01/05 10:30 AM
by kungpow
* Using pint jars instead of half Juice7 454 2 04/30/03 10:41 PM
by Skikid16
* Perilite instead of verm in brf cakes?
( 1 2 all )
habitat0789 2,319 25 01/08/07 09:46 PM
by Implosive

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,401 topic views. 26 members, 238 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.011 seconds on 15 queries.