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OfflineTreycherry
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Spore syringe to agar
    #26960844 - 09/29/20 06:46 PM (3 years, 3 months ago)

A few days ago I put some Fiji spores to agar via multi spore syringe.
My question is where do I even start when it comes to isolation? What should I look for to use as my first transfer. I see some stringy mycelium and also some very puffy kinds too.



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Offlineseand04
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Re: Spore syringe to agar [Re: Treycherry]
    #26960953 - 09/29/20 07:46 PM (3 years, 3 months ago)

Both plates have some nice mycelium you can transfer to a new plate


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InvisibleMateja
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Re: Spore syringe to agar [Re: Treycherry]
    #26961011 - 09/29/20 08:26 PM (3 years, 3 months ago)

Quote:

Treycherry said:
What should I look for to use as my first transfer?



For the first transfer I'd try to only grab the myc growth and avoid grabbing parts of the inoculation spot itself which will contain spores and what not. Just make sure the parts that you grab look like they contain only one kind of organism on it. Try to avoid parts that have two or more different growth characteristics. If my agar puck is kind of thick I'd try to 'scoop up' the myc with the tip of the blade, if my agar puck is relatively thin is try to just cut out a rice grain sized wedge. Gl


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InvisibleRafiikii
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Re: Spore syringe to agar [Re: Mateja]
    #26961072 - 09/29/20 08:55 PM (3 years, 3 months ago)

wait a few more days and pick a promising spot, can definitely transfer now but waiting will give you a better visual


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