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OfflineBucknaztee
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Registered: 11/21/09
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Liquid Culture jar issues
    #26960673 - 09/29/20 04:36 PM (3 years, 3 months ago)

Hey all,
    Just went to make 2 LC's from spores, and I had an issue that keeps popping up. I have been using plastic lids with SHIP's, 1-8 ports respectively, lol different story. There is also a 1 cm. hole in the middle with a tyvek filter on the underside of the lid. The jars themselves are 1/2 pint wide mouths filled with 150 ml. of H2o and 6 gm. honey/karo. This means the liquid is close to the filter/top of jar, and though I tested this last night with plain H2o in a dummy jar,injecting 3 cc.'s of solution in them today and liquid bubbles through a port or through the filter, and at that point I can only assume this isn't going to work out. I do it this way so I can extract the LC without opening the jar, as i use old spore syringes to extract.
    So is there a longer needle that I can find to fit on the syringe, or can I use a vessel that is larger,with more space between the top of the liquid and the lid, and just open it without fear of contamination?
Do I need a filter on these even? Any help would be appreciated, I'm getting really frustrated with LC's and agar lately. Thank You.


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Offlineshroomshine
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Re: Liquid Culture jar issues [Re: Bucknaztee]
    #26960697 - 09/29/20 04:51 PM (3 years, 3 months ago)

I am not an expert but made a handful (or two) LCs myself which I have used with great success..so here are a couple thoughts which might help you out...

- spores to LC is a bad idea, since spores are rarely sterile. you mentioned agar in your post so I would recommend using Josex poke tek (biopsy method) to inoculate your LC
- I am seeing faster growth with LME (2-4g/L) vs. Karo/potato flakes(2g/L each) but once colonized both works fine ime, honey actually (aahktuaalie) has anti fungal properties
- you can use any size jar you want
- LCs need less GE than you think
- test your LC on agar before use
- pour your LC (in SAB or in front of hood) vs using a syringe (it's kinda hard to suck liquid out of a closed jar plus you add a contamination factor by using a port)
- yes, there are longer (3") needles
- don't give up -> LCs are freaking awesome when they work out


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Edited by shroomshine (09/29/20 04:56 PM)


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OfflineBucknaztee
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Re: Liquid Culture jar issues [Re: shroomshine]
    #26960710 - 09/29/20 05:04 PM (3 years, 3 months ago)

Concise and to the point, unlike this post lol, thank you. Yeah I just started my foray into agar because I know LC's are unpredictable using MS solutions (Though when I DO get them to work, they are awesome). Tried live tissue in LC jar via biopsy, but no growth. And I don't want to pour LC in PF jars...Or do I (I use PF tek but with tyvek filters and SHIPs instead of traditional verm. barrier). Thanks for all your tips friend, no giving up yet!


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InvisiblemushboyMDiscord
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Re: Liquid Culture jar issues [Re: Bucknaztee]
    #26960717 - 09/29/20 05:08 PM (3 years, 3 months ago)

Spores to lc is predictable:lol:

I've poured lc to pf style jars they actually suck it up nicely:thumbup:


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OfflineBucknaztee
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Re: Liquid Culture jar issues [Re: mushboy]
    #26960785 - 09/29/20 06:04 PM (3 years, 3 months ago)

Mushboy what you mean? What is this pouring? Do you makes PF jars with no verm barrier like me and then just dump like a little bit in and seal her back up? Thanks.


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Offlineseand04
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Re: Liquid Culture jar issues [Re: Bucknaztee]
    #26960813 - 09/29/20 06:33 PM (3 years, 3 months ago)

I made a LC from a PE clone onto agar. It colonized up nicely.  Then I put it into syringes. Working nicely.  The LC mix I used distilled water, turbinado sugar, and brown rice flour, I added a little food coloring. I inoculated 50 jars about 5 days ago, looking awesome. Almost ready for coir. I changed from metal lids. To plastic lids. I don't like the rust.


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InvisiblemushboyMDiscord
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Re: Liquid Culture jar issues [Re: Bucknaztee]
    #26960815 - 09/29/20 06:33 PM (3 years, 3 months ago)

Yup you got it:thumbup:

Click the go insane with lc link below:awesomenod:


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OfflinePinkey
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Re: Liquid Culture jar issues [Re: Bucknaztee]
    #26960823 - 09/29/20 06:36 PM (3 years, 3 months ago)

I pour LC to grain jars.



Some PE jars roughly a week or so after pouring.





I go agar>LC in media bottle> grain jars. It is my preferred method.

Mushboy has a whole thread on it...that's were I got the idea.

Thanks Mushboy !!!


--------------------
"Looky here, baby... You're hittin' them cornaz too goddamn fast.  You need to slow this muthafucka down.  Ya understand?  I almost spilt my yack on this $200 suit, nicca.  C'mon, baby, get it togetha."

Pinky



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InvisiblemushboyMDiscord
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Re: Liquid Culture jar issues [Re: Pinkey]
    #26960827 - 09/29/20 06:37 PM (3 years, 3 months ago)

I got the idea from josex who I think got the idea from an og named TL.


Edited by mushboy (09/29/20 06:39 PM)


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OfflinePinkey
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Re: Liquid Culture jar issues [Re: mushboy]
    #26960833 - 09/29/20 06:40 PM (3 years, 3 months ago)

Quote:

mushboy said:
I hot the idea from josex who I think got the idea from an og named TL.




It's da bomb...super effective...and super simple.


--------------------
"Looky here, baby... You're hittin' them cornaz too goddamn fast.  You need to slow this muthafucka down.  Ya understand?  I almost spilt my yack on this $200 suit, nicca.  C'mon, baby, get it togetha."

Pinky



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InvisiblemushboyMDiscord
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Re: Liquid Culture jar issues [Re: Pinkey]
    #26960834 - 09/29/20 06:41 PM (3 years, 3 months ago)

Hells ya:rockon:


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Offlineseand04
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Re: Liquid Culture jar issues [Re: mushboy]
    #26960846 - 09/29/20 06:46 PM (3 years, 3 months ago)

This is one of the LC syringes. The mycelium stays in the middle like a log:aweohyou:


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OfflineFripp
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Re: Liquid Culture jar issues [Re: seand04]
    #27105927 - 12/24/20 02:19 PM (3 years, 1 month ago)

did you mean it was a liquid culture with flour? does it work like lme light malt extract?


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