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OfflineTreycherry
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Registered: 07/30/20
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Mixing verses layering
    #26959971 - 09/29/20 09:16 AM (3 years, 3 months ago)

I used a full agar tray on 2 wbs jars.
Once they fully colonized I put them in 2 separate tubs.
One was mixed and one was layered.
The layered one showed signs of colonization way faster initially and still has more than the mixed tub but I will say I’ve noticed the mixed tub is beginning to catch up very fast.

Here are both tubs after only 3-4 days of being moved to the fruiting tubs.



First picture- layered
Second picture - mixed
Third picture -  mixed and then layered
Fourth picture - mixed and then layered


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OfflineKmacmo
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Registered: 08/14/19
Posts: 1,675
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Re: Mixing verses layering [Re: Treycherry]
    #26960051 - 09/29/20 10:08 AM (3 years, 3 months ago)

They look great, I've never tried layering them but I would think it doesn't make too much difference


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OfflineTreycherry
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Re: Mixing verses layering [Re: Kmacmo]
    #26960149 - 09/29/20 11:16 AM (3 years, 3 months ago)

Some people swear by both. I always hear mixing is best but with my trial run of both the layering was best


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OfflinePearl
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Registered: 05/28/20
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Re: Mixing verses layering [Re: Treycherry]
    #26960259 - 09/29/20 12:29 PM (3 years, 3 months ago)

How thick were the layers - especially the final layer?

You can't really tell but it kinda looks like the mixed is colonizing faster through the sub given the area of the front we can see. But the layered took the top faster. Who knows how those tubs compare on the inside?

Probably just personal preference as long as it's evenly mixed OR layered really...

But look good regardless :smile:


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InvisibleRoger Clemency
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Registered: 03/23/20
Posts: 2,005
Re: Mixing verses layering [Re: Pearl]
    #26960287 - 09/29/20 12:41 PM (3 years, 3 months ago)

I think the most frustrating thing in this hobby is the insane difference in what works the best for who, when, and for what.

You might do clone testing for coir vs hpoo, and coir wins out on several tests with that clone. For someone else hpoo will rock the coir because their clone likes hpoo more for whatever reason. Everyone’s grow room conditions are different so one hole setup will be perfect for this person and suck for another.

It’s all about what works best for you, with the thing that you’re growing. I don’t think there’s a right answer anywhere except that you should clean cultures on agar before going to spawn. It’s all advice to get your in the ball park and then it turns individual.


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Sour grapes, sweet revenge
Heaven starts right where hell ends


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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Mixing verses layering [Re: Roger Clemency]
    #26960421 - 09/29/20 02:22 PM (3 years, 3 months ago)

It's best to mix evenly IMO with a lower spawn ratio.  When you're approaching 1:1 it won't matter one bit.  If your spawn is clean and the ratio isn't ridiculously low, it doesn't matter much.  Logic dictates that mixing spawn evenly spreads out inoculation points more evenly, resulting in slightly faster total colonization.  You'd have to run multiple isolates in a controlled environment to get any real breakdown.
Clean spawn/a healthy culture and good sub prep matters way,  way more though.  Just my 2 cents.


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"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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