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OfflineMeNtALDeMoNZ
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Registered: 05/09/04
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newbie question 'bout CC
    #2695977 - 05/18/04 12:44 PM (19 years, 10 months ago)

Can one use a "finished" pf cake to spawn a jar of Cracked Corn???
or it that asking for contams???
Thanx, MD

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InvisiblePrisoner#1
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Re: newbie question 'bout CC [Re: MeNtALDeMoNZ]
    #2695986 - 05/18/04 12:45 PM (19 years, 10 months ago)

you may be able to use some from the center of the cake, you'll need a flow hood and peroxide will be a must....

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OfflineMeNtALDeMoNZ
#1 Dung SproutConsumer

Registered: 05/09/04
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Last seen: 19 years, 8 months
Re: newbie question 'bout CC [Re: Prisoner#1]
    #2695990 - 05/18/04 12:46 PM (19 years, 10 months ago)

whats the peroxide for???

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OfflineMeNtALDeMoNZ
#1 Dung SproutConsumer

Registered: 05/09/04
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Re: newbie question 'bout CC [Re: Prisoner#1]
    #2695996 - 05/18/04 12:47 PM (19 years, 10 months ago)

no flow hood but i got a glove-box?!?

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InvisiblePrisoner#1
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Re: newbie question 'bout CC [Re: MeNtALDeMoNZ]
    #2696009 - 05/18/04 12:50 PM (19 years, 10 months ago)

a glovebox can also work pretty well... follow normal cleanroom steps, use a sterilized knife (alcohol) and slice away the outside of the cake... I usualy cut it square... after you break up the cake dip the pieces in H2O2 (peroxide) and drop them into your fresh substrate jars...

you have a PC for the cracked corn?

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OfflineMeNtALDeMoNZ
#1 Dung SproutConsumer

Registered: 05/09/04
Posts: 78
Loc: InYourFace!!!
Last seen: 19 years, 8 months
Re: newbie question 'bout CC [Re: Prisoner#1]
    #2696025 - 05/18/04 12:53 PM (19 years, 10 months ago)

no pc prisioner#1 but i took the nessasary step of fracual sterilization,,,, thanx for the info

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OfflineMeNtALDeMoNZ
#1 Dung SproutConsumer

Registered: 05/09/04
Posts: 78
Loc: InYourFace!!!
Last seen: 19 years, 8 months
Re: newbie question 'bout CC [Re: Prisoner#1]
    #2696331 - 05/18/04 01:46 PM (19 years, 10 months ago)

aaa-right prisoner#1 he did everything you said,hopefully it will work out, butchered 2 cakes just to speed things up a bit, didnt look like there was enough myc. in there!
thanx for all the info, MD

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