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nektar61
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CRISPR for editing shroom DNA
#26959697 - 09/29/20 03:54 AM (3 years, 3 months ago) |
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I'm not the person to do this. I like reading about science and watching videos about it, but I don't know much about the practice of doing science.
But from reading posts here, there might be someone who can do it.
but there's a DIY CRISPR gene editing kit for 160 USA dollars: https://www.the-odin.com/diy-crispr-kit/
This was brought up here 3 years ago. But pinging because the tek has gottn better, cheaper, and there is a lot more info online now. Plus there might be someone new here who could do it who wasn't here 3 years ago.
That kit might be just an educational thing for young people. But I think the real tek is doable for someone with a little money, maybe a job.
Possibilities? Maybe actually crossing two species. Maybe making yeast create psilocybin so it's easy to grow. I heard that's a thing in a lab somewhere at a pharmaceutical company.
I don't know. I'm not the scientist here. But some of you are.
I believe some of you can do it!
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MolecularConcept
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Re: CRISPR for editing shroom DNA [Re: nektar61] 1
#26959873 - 09/29/20 08:14 AM (3 years, 3 months ago) |
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if i remember i thought they already got a yeast to produce psilocybin. it would be awesome if i could do it from home!
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nektar61
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Quote:
MolecularConcept said: if i remember i thought they already got a yeast to produce psilocybin. it would be awesome if i could do it from home!
It has been done with both yeast: https://newatlas.com/science/psilocybin-synthesis-yeast-bacteria/
and e-coli: https://psilocybin.net/e-coli-ppsilo16-psychedelic-drug-strain/
Anyway, I think they said it cost way more than just growing shrooms.
But I'm thinking "Why does it have to?" and "Maybe that's because a large pharmaceutical company WANTS it to cost way more than growing shrooms."
...And then the company could use their lobbyists to force laws to make that the only legal version that version....Like happened with Marinol (pharmaceutical synthetic marijuana for AIDS wasting, and glaucoma). https://www.drugs.com/marinol.html
Marinol is still the only legal marijuana medicine a doctor can prescribe in some places. Costs more than pot and doesn't have any CBD or entourage effect either, because is missing the other plant components.
But I'm thinking if DNA alteration has been used to make psilocybin yeast AND from e-coli, two very different organisms, it can't be that hard for someone with genetic science experience and a little money for gear to pull it off.
Or just the gear, smarts, and an internet connection.
I'm not the lady to do this, but I will cheer on anyone who can do it.
Could really make psilocybin unstoppable. Might do with it what they said nuclear power plants would do with power. "Make it too cheap to meter." lol.
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j.dob
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Re: CRISPR for editing shroom DNA [Re: nektar61] 1
#26964584 - 10/01/20 07:08 PM (3 years, 3 months ago) |
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Yeast is easier to grow. After rapid climate warming there are areas left with no leftover 20 degree fruitation temperature. Like California when there's 50 degree celsius in hottest season. Therefore yeast is easier way to do so.
I think it has been university research not pharmaceutical company. They would synthesize it from indole by microwave chemistry.
My opinion in general is that we should somehow, or somebody if not we, but I think it counts, should engineer it into gut bacteria and deliver it as probiotics to general population.
According to my calculations, it would be like permanent microdosing.
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nektar61
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Re: CRISPR for editing shroom DNA [Re: j.dob]
#26964659 - 10/01/20 07:43 PM (3 years, 3 months ago) |
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Quote:
j.dob said: Yeast is easier to grow. After rapid climate warming there are areas left with no leftover 20 degree fruitation temperature. Like California when there's 50 degree celsius in hottest season. Therefore yeast is easier way to do so.
I think it has been university research not pharmaceutical company. They would synthesize it from indole by microwave chemistry.
My opinion in general is that we should somehow, or somebody if not we, but I think it counts, should engineer it into gut bacteria and deliver it as probiotics to general population.
According to my calculations, it would be like permanent microdosing.
I like it.
A pre-pro-drug. or A pro-pro-drug.
Sounds good, since I am pro-drug.
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nmd_myco
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Re: CRISPR for editing shroom DNA [Re: j.dob] 1
#26964697 - 10/01/20 08:11 PM (3 years, 3 months ago) |
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Quote:
j.dob said:
My opinion in general is that we should somehow, or somebody if not we, but I think it counts, should engineer it into gut bacteria and deliver it as probiotics to general population.
I make probiotic salt-brine pickles. Teaming with lactos. Somebody needs to make my pickles team with psilocybin. Let me know when you figure it out.
Trippy Pickles (tm) here I come.
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nektar61
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Re: CRISPR for editing shroom DNA [Re: nmd_myco]
#26964789 - 10/01/20 09:15 PM (3 years, 3 months ago) |
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Quote:
nmd_myco said:
Quote:
j.dob said:
My opinion in general is that we should somehow, or somebody if not we, but I think it counts, should engineer it into gut bacteria and deliver it as probiotics to general population.
I make probiotic salt-brine pickles. Teaming with lactos. Somebody needs to make my pickles team with psilocybin. Let me know when you figure it out.
Trippy Pickles (tm) here I come.
lol.
Or call it "Pickle Trip." (Rick and Morty fans will get it.)
Have you pickled shrooms yet? Doesn't sound inviting to me, but it might have its place somewhere in the world.
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CouchShroom
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Re: CRISPR for editing shroom DNA [Re: nektar61]
#26976205 - 10/08/20 09:49 PM (3 years, 3 months ago) |
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You actually don't need CRISPR at all to make the psilocybin producing E. coli, those experiments were all done with traditional molecular cloning techniques and expression plasmids.
The question of why you would bother doing this at home is definitely something to think about here. This sort of development is important for pharmaceutical companies because they can get these bacteria to pump out a bunch of psilocybin, which presumably can then be purified and aliquoted into precise and consistent doses. That's an extremely important point whenever you're talking about some kind of natural product being transitioned into a quality controlled pharmaceutical product. Doctors aren't going to be happy giving their patients the kind of dosing guidelines that are often posted here... we all know that different batches of mushrooms can have different potencies. What happens if you prescribe a patient a light dose of "2 grams of cubensis", but they happen to get an extremely potent batch of mushrooms their first time around? Now your patient is on Saturn with the sand worms and is extremely unhappy with you . It's a liability for them.
Unless you care about having that kind of precision (and you're able to purify and quantify the final product from the E. coli culture) its much simpler and cheaper to just grow the mushrooms.
One project I've been thinking about that could potentially use CRISPR is comparing the genomes of P. cubensis and P. cyanescens, and trying to identify what's different about the psilocybin biosynthesis gene cluster in the Pans that makes those mushrooms so much more potent. If you could figure that puzzle out, maybe you could find a way to edit the cubensis genes to be more like the cyanescens ones. End result would ideally be a mushroom with the potency of P. cyanescens, but be as easy to grow as any other cubensis strain.
Both genomes have been published, so the comparative genome analysis part is definitely doable: https://onlinelibrary.wiley.com/doi/full/10.1002/evl3.42. Still, it would be a lot of time and money invested into solving a pretty trivial problem... Pans aren't THAT difficult to grow.
Edited by CouchShroom (10/08/20 09:51 PM)
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ZionsMane
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Re: CRISPR for editing shroom DNA [Re: CouchShroom]
#26978426 - 10/10/20 12:01 PM (3 years, 3 months ago) |
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As per E.Coli producing psilocybin; I think that there is something to the presence of psilocin as well as other compounds in a natural mushroom that assist with some of the experiences we like from these organisms.
I'd be interested to see what type of an experience one would have with isolated psilocybin. There is a 'product' I see pop up occasionally where I live called "goo" and it comes looking like a tootsie pop. I think it is some sort of extraction technique from the cannabis industry but I personally feel that it is different than eating whole fruits, I dunno.
Does anyone know about this 'goo' and how it is made?
Or if my theory (based on absolutely no scientific research) about psilocin makes sense?
The times I've tried goo feels like a diet trip.
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mycot
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Re: CRISPR for editing shroom DNA [Re: ZionsMane]
#26982034 - 10/12/20 05:56 PM (3 years, 3 months ago) |
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I'm really not keen on genetic modification for many reasons because of possible unforseen dangerous consequenses.
That said I still approve of scientific research in genetics. Just not its hasty commercialization and application to food and exposure to the environment.
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