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OfflineEp1429
Stranger
Registered: 10/10/18
Posts: 800
Last seen: 2 years, 11 months
Re: Help with bacterial contaminant [Re: Bloo]
    #26935505 - 09/14/20 04:13 PM (3 years, 4 months ago)

Quote:

Bloo said:
I think it's bacterial, it grows on bacterial screening media.

Fully colonized quarts, grown on oats, are spawned into plastic tubs (dishwashered and wiped down with isopropyl, allowed to dry in a flow hood), and broken up with a sterile tool. I never touch anything with bare hands, never add just oats, only colonized grain. Sometimes there's a casing layer, sometimes not, there's no difference.

The cubensis grows just fine, but there's large blocks of oats that refuse to colonize, they turn dark brown, look kind of wet, and never fully colonize- again, this is from fully colonized quarts.

Once I grabbed a few of the suspect patches and put the grains on bacterial screening media and they grew bacteria, sure enough, I forget the odor but it wasn't remarkable.

The grains kind of colonize but they don't colonize completely, leaving large patches like this one without fungal growth. The containers never fruit, they never contract and pull away from the walls, they just sit there forever, doing nothing.

It doesn't matter if I add water or not after spawning them, they're pretty dry when the grain comes out of the quarts already.

What am I doing wrong?





</font>




You’re describing the same problem I’m having.  I’m running a grow log on it in that section, feel free to follow along.


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OfflineBloo
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Registered: 02/24/20
Posts: 56
Last seen: 1 year, 9 months
Re: Help with bacterial contaminant [Re: Lieutenant Pan]
    #26958226 - 09/28/20 01:50 AM (3 years, 4 months ago)

Quote:

Lieutenant Pan said:
Try pasteurizing some organic coco coir from the large gardening bags.  Basically mix 1 cup of colonized grain to 4 cups of coir.  With B+ a casing won't matter.




OK, I tried this (and a bunch of other stuff), and- holy shit.



That's just 11 days after mixing colonized grain with PC'd coir.

I'm sure I got the original idea of colonized grain + PC'd grain from a tek somewhere or one of the books I read, but now I think I get it. Anyway, thank you, everyone for your patience with me, and particularly Lieutenant Pan whose advice was spot on. I honestly didn't think it would work, but tried it anyway in duplicate and that one is doing good too.

And I think I figured out the contaminant.


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OfflineLieutenant Pan
Stranger

Registered: 09/11/20
Posts: 339
Last seen: 4 months, 30 days
Re: Help with bacterial contaminant [Re: Bloo]
    #26959646 - 09/29/20 01:47 AM (3 years, 4 months ago)

That's good news what you did is "spawn to bulk" so read up on that and you'll know what's up.  Once you master these look into wood and dung lovers. 


--------------------
I never thanked ya fer saving my life.


Edited by Lieutenant Pan (09/29/20 02:02 AM)


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