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Doctor Mario
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Critique My Technique 2
#26959523 - 09/28/20 10:21 PM (3 years, 3 months ago) |
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I believe that there's always room to improve and sometimes it's easier to see while looking from the outside in. I think this thread could be beneficial for anyone looking for advice. Cultivators with great experience are always trying to diagnose what exactly went wrong with another person's grow and help them succeed but lets be honest, pictures can only show so much. If you've hit a rock that you just cant get past or decide you want someone to critique your technique, please feel free to comment with a video of your process.
I normally have two or three out of 20 plates contam while pouring and another two or three while transferring (video to come). Making this video made me realize that I occasionally get shaky hands. Besides that, is there anything else that I'm doing subpar?
What isn't shown is everything before the video. What I did prior was: lay a wet towel (soapy water) down on the table, and spray soapy water on the inside of the SAB. Next, I sprayed a few mists of the same soapy water into the air of the SAB. After a few minutes, I took a few stacks of blank petri dishes, sprayed them with ISO and carefully put them in position on the towel. Afterwards, I sprayed a new sleeve of petris with ISO, moved it into the SAB and carefully opened the sleeve and placed the stacks ontop of the blank dishes. Finally, I sprayed a few more mists of soapy water into the air of the SAB and went to work. Heres the video. What can I do better next time?
Edited by Doctor Mario (09/28/20 11:05 PM)
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NothingsChanged
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Way to go brother.
Is that a fan running in back ground?
May want to loose the sweat shirt/long sleeves?
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Doctor Mario
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Thank you. All windows are closed and AC is shut off an hour prior to working. What youre likely hearing is the noise of the plates rattling from my ridiculously shaky hands. I'm not usually this shaky while working. I set the box up a little bit too far away from me this time making the pour sesh feel a little awkward. I think I was also a bit nervous knowing that I was making a video for everyone to see.
I've only just started wearing long sleeve shirts because the contams I see the most are bacterial. Sometimes I get mold but its a far lower rate than the off white/yellow blobs that I see. I assumed it's probably from skin cells coming off of my arms.
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Inthepit
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Quote:
NothingsChanged said: Way to go brother. Is that a fan running in back ground? May want to loose the sweat shirt/long sleeves?
I'm confused about security, YouTube, and arm tats, so sleeves. But only agar is being poured...still makes me nervous.
So noob comment, when I pour, I don't bring the bottle all the way back. I'm thinking sloshing and airflow going in and out of the media bottle.
Looks great, I really would like to video, but...YouTube/Google.
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Doctor Mario
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Re: Critique My Technique [Re: Inthepit]
#26959762 - 09/29/20 06:04 AM (3 years, 3 months ago) |
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Quote:
Inthepit said: I'm confused about security, YouTube, and arm tats, so sleeves. But only agar is being poured...still makes me nervous.
So noob comment, when I pour, I don't bring the bottle all the way back. I'm thinking sloshing and airflow going in and out of the media bottle.
Looks great, I really would like to video, but...YouTube/Google. 
I wasn't going to mention it but that's also why I wore sleeves. Im literally covered in tats. I'm less paranoid about a governmental agency seeing my vids and more paranoid that someone I know will see them.
I honestly didn't even think about what could happen by bringing the bottle back... Thank you! Comments like that are exactly what I'm looking for.
I'm not sure how important it probably doesn't hurt to strip the metadata from your videos before uploading to YouTube. Everything I'm doing here is for fun and personal experience. It will never be to make a profit and that's what really matters. The government doesn't give a shit what you do unless your making money and they aren't getting a piece of the pie. Even with metadata being stripped from videos and photos, there's always a way they could find you if they wanted to. At that point, we're already fucked anyway. I think you're much more likely to have a bad time if someone that you know says something or sees you in a video or pictures.
Edited by Doctor Mario (09/29/20 06:20 AM)
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MLPismyOPSEC
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Technique looks pretty good, i really only have minor suggestions so here's what i would change. Use a smaller paper towel to wipe down the bottle, like half the size you used. That might be stirring up the air. Use gloves one size smaller, the bunched up ridges could be messing with your tactile sense, and using a slightly too small size would help. I wasn't real clear on the video, but pay attention to where your fingers are on the lid vs where they hover over the plate. I try to grab the lid somewhere between 12-and-6 and 11-and-7, and move it strictly towards 9 o'clock so my fingertips aren't over the open plate, pour at 3 o'clock. Think of everything like you were looking top-down, and limit any non-sterile item going over any sterile item as much as you can possibly manage. Eg. when i pour, i barely have the drip ring hanging over the open plate.
Rereading your post, A) do you spray your petris with iso after opening the sleeve? B) do you spray your SAB with stacks of petris in it? Theoretically that shouldn't cause issues, but practically, i wouldn't recommend that. Petris are sterile in the sleeve (i know you know this, not being condescending) so introducing anything, even iso, is making them less sterile and spraying iso inside the box is definitely stirring the air.
I started out hovering around 14-16% contam rate, and worked down to <1%, took me about 750 petris. I know how frustrating it can be, but if you keep track of your contam rate and little changes in your technique, it's extremely satisfying to get that rate down. Good luck man! You've got this
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Doctor Mario
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Quote:
MLPismyOPSEC said:
Rereading your post, A) do you spray your petris with iso after opening the sleeve? B) do you spray your SAB with stacks of petris in it? Theoretically that shouldn't cause issues, but practically, i wouldn't recommend that. Petris are sterile in the sleeve (i know you know this, not being condescending) so introducing anything, even iso, is making them less sterile and spraying iso inside the box is definitely stirring the air.
I dont spray the plates with iso but I do spray a few mists of soapy water in the box after I put them in there. I take the stack out of the sleeve inside the box and it creates a lot of air movement. I was concerned with stuff floating around and decided that a few mists probably wont hurt but I could very well be wrong.
I like the description you've given on the proper way to hold the plate. I'm taking notes on all advice that is being contributed in this thread and I will definitely do some practice plates tonight. I've only poured 90 plates up to this point and I appreciate the fact that just maybe with your help, it wont take 750 to see a 1% or less contam rate.
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tryptkaloids
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I would omit the spray after removing the plates from the sleeve. Just let the air settle naturally.
Is there any reason you didn't pour the whole stacks?
It might sound counter intuitive, but when I pour i try to keep the empty plates on top completely off to the side, not over the plate im pouring, that way when I shake anything that made it on the outside doesn't fall into the plate
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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dirtybirdx
getting better

Registered: 07/28/20
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If you have stacks of just 9-10 like that - pour from the bottom up. I know it seems crazy at first to balance 10 plates but the point is that your hand goes on the stack one time, and then comes off it once the stack is done - minimal handling.
Once you do it a few times itβs not even difficult and when youβre going quickly it only takes a few seconds to get through those first few plates to where the stack feels secure in your hand again.
And you need a rack to raise your work area off the floor of the SAB, especially when working with plates, because their sides are only 15mm high. Not as necessary if youβre only working with jars.
As far as your sleeves go, hereβs what I do: Iβm always sleeveless, but I have the bowl of soapy water I use to prep my SAB next to me. Whenever I work, I make sure my forearms are soaped up to my elbows. Now when I put my arms in, any airborne contams that come into contact with them get stuck, and donβt fall into my work.
Every 15 minutes when my arms dry, I reapply. Just like I reapply soapy water to the inside of the SAB every 35 mins or so once it dries, if Iβm doing a longer session.
Thatβs just my .02, small stuff.
Looks good π
-------------------- Need help?

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tryptkaloids
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Re: Critique My Technique [Re: dirtybirdx]
#26961286 - 09/30/20 12:25 AM (3 years, 3 months ago) |
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Yea a cookie cooling rack is a good idea
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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NedRise
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Quote:
Doctor Mario said: Finally, I sprayed a few more mists of soapy water into the air of the SAB and went to work.
What do you hope to gain from that procedure? If you want to wash the air, wouldn't Listerine be more fitting as it also works for breath?
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Inthepit
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Quote:
tryptkaloids said: Yea a cookie cooling rack is a good idea
Ya, son of a gun missed it. And I'll plug my new deal  I would have a clorox damp towel under the rack too, but I was just demonstrating.
Edited by Inthepit (09/30/20 05:54 AM)
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Doctor Mario
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Re: Critique My Technique [Re: NedRise]
#26961508 - 09/30/20 06:36 AM (3 years, 3 months ago) |
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Thats all solid advice. I appreciate all the input. I havent found a cooling rack thats quite the length and width I'm looking for. Instead, I've just been using a few blank petri dishes to keep my work a bit higher than ground level. That's why it looks like I'm not pouring the whole stack. Those dishes were used once, cleaned out and wrapped with saran wrap to hold them together.
Quote:
NedRise said:
Quote:
Doctor Mario said: Finally, I sprayed a few more mists of soapy water into the air of the SAB and went to work.
What do you hope to gain from that procedure? If you want to wash the air, wouldn't Listerine be more fitting as it also works for breath?
The theory behind spraying a few mists of water in the SAB is to catch any mold spores that are still floating around and bring them to the floor. Mold spores cant be killed by IPA so I doubt that listerine would either. IPA can actually become contaminated. Just somethin to think about.
I like the idea of keeping your scalpel in the box and heating the blade from the outside. Looks like I'll be putting another hole in my SAB tonight. Thank you!
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Inthepit
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Quote:
I like the idea of keeping your scalpel in the box and heating the blade from the outside. Looks like I'll be putting another hole in my SAB tonight. Thank you!
Oh hot dang! Yes I'm hoping more peeps try it. I spent some time going thru the motions of where the scalpel felt best to exit the SAB.
Then I almost did not allow for the distance from the torch to the actual hot spot of the flame. So far it feels comfortable and with practice I think it'll be a winner. The main point being less air disturbance going in and out of the SAB.
Wow! Wish I had tried the hot coffee can before. The little tomato paste can went thru like butter!
Edited by Inthepit (09/30/20 07:07 AM)
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NothingsChanged
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Re: Critique My Technique [Re: Inthepit]
#26961700 - 09/30/20 09:50 AM (3 years, 3 months ago) |
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Couple thought, iso vapors very flammable coming out of your new hole is a possibility.
Blowing hot air across your new hole could be pulling air in through your arm holes.
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Inthepit
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Thanks NC, I'll take your valid concerns and start a hole in the SAB thread. side hole This is the good Doctor's show.
Uh, if I don't blow up first !
Edited by Inthepit (09/30/20 07:18 PM)
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Doctor Mario
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Re: Critique My Technique [Re: Inthepit]
#26962473 - 09/30/20 05:15 PM (3 years, 3 months ago) |
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Quote:
Inthepit said: Thanks NC, I'll take your valid concerns and start a hole in the SAB thread. This is the good Doctor's show.
Uh, if I don't blow up first !
Nonsense, anyone is welcome to carry on conversation here as long as it pertains to the title. You shared, your idea and someone critiqed it. Although, you will probably get more feedback by starting a thread about it.
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Inthepit
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CocaineBuffet
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Re: Critique My Technique [Re: Inthepit]
#26962651 - 09/30/20 07:07 PM (3 years, 3 months ago) |
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Your technique looks good to me! Steady and slow movements. The way I work in my SAB I have similar success rate but I have to admit I am heavy handed with most things in this hobby.
I think you may be over sanitizing perhaps. I think most members buy sterilized petri dishes (come in vacuum sealed package). I just cut them out of the package and put them into the SAB and don't spray them. Also if you clean your SAB and go to work immediately you may have not allowed enough time for the air to settle and have too much floating around in the space.
If nothing else hopefully my suggestion can help you cut down on using too much on soap or ISO.
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brad81684
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Re: Critique My Technique [Re: Inthepit]
#26962655 - 09/30/20 07:13 PM (3 years, 3 months ago) |
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I use a couple of old petris seran wrapped together as my floor for fresh plates. Pour bottom to top, also I wipe the bottle down outside of the box and put it in. Wiping inside is going to move some air. Sleeves are optional, I dont wear any or soap my arms, to each their own. Your movement seems fine and you'll get better as you go. Before you know it, you'll be doing stacks of 15 in minutes.
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Doctor Mario
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Re: Critique My Technique [Re: brad81684]
#26963015 - 09/30/20 10:18 PM (3 years, 3 months ago) |
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More suggestions added to the list. 
Thank you.
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Inthepit
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Speaking of lists Doc here's mine...so far:
Washed 90 Qrt SAB, rack, and table, w/Clorox & towel Books on top of SAB to anchor it down and reduce effects of bumping. Nitrile gloves, mask, glasses, hat, iso gloves, (forearms soaped). Wet towel under 3β high rack. Lysol wipes under plates iso the scalpel handle before Hold scalpel toward the end try flaming the entire blade all the way down to where it locks on. Move slowly, donβt disturb the air Lift petri dish cover very slowly Move stack completely away from being over the bottom plate so fingertips aren't over the open plate. don't bring the media bottle all the way back when pouring. Don't bother cooling the blade, be the sizzle! work at the back of the SAB
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Doctor Mario
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Re: Critique My Technique [Re: Inthepit]
#26977304 - 10/09/20 04:44 PM (3 years, 3 months ago) |
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Just popping in here for an update on those plates I poured. 16 out of 20 grew mold, bacteria or both.
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JHOVA
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Try no pour.
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π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Doctor Mario
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Re: Critique My Technique [Re: JHOVA]
#26977339 - 10/09/20 05:06 PM (3 years, 3 months ago) |
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Thats my next plan of action. I enjoy pouring and really want to get it right.
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JHOVA
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I recommend the holy grail containers. The cheaper ones for $20 on amazon are better bang for your buck.
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Doctor Mario
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Re: Critique My Technique [Re: JHOVA]
#26977359 - 10/09/20 05:22 PM (3 years, 3 months ago) |
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Noted. Thank you, sir. On a more positive note, I came back today from a week long business trip and have some oat jars that I believe are 80-100% colonized.

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NothingsChanged
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Something must be up, seems like a high contam rate?
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Doctor Mario
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Quote:
NothingsChanged said: Something must be up, seems like a high contam rate?
I agree. This is the highest contam rate yet. Usually its only 2 or plates that contam from a pour session. Havent had that many go wrong since my first pours. Theres a lot of mold in this area. The dirt outside has a green hue and algae/mold grows on everything. Im sure we bring it in the house with is everything we go inside. Maybe I'm not moving slow enough in the SAB?
I dropped a wedge on accident and it fell on the wet towel. I grained it anyway and the jar went massive trich. Im gonna stop using the towel and see if things get any better.
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tryptkaloids
Learner



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Wash your carpets, scrub your sab. Pc your tools.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Doctor Mario
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I clean my SAB before every use. No carpets, just hardwood floors and it probably wouldn't hurt to PC some tools.
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TheBoJim
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Quote:
Doctor Mario said: Just popping in here for an update on those plates I poured. 16 out of 20 grew mold, bacteria or both.
I am about to start pouring my own for the first time. I have good luck with clones and transfers so far using premade plates. Lost only 3 to contam out for 40.
I don't use a towel or soapy water. Just the lid of the sab an a bunch of iso.
However lately I have been having trich issues in my tubs. I believe the cause is me doing G2G using quart jars to quart jars. Too big for my small SAB. I end up bumping the top of the box by the jar. Bigger box and/or smaller master jars are needed.
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Funky Monkey
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This is a great idea for a general help thread for everyone!
A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.
Visual learners rejoice!
If you don't start it Mario, I will. Say the word.
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Doctor Mario
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Quote:
Funky Monkey said: This is a great idea for a general help thread for everyone!
A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.
Visual learners rejoice!
If you don't start it Mario, I will. Say the word. 
That was the intention of the thread but it never kicked off. A lot of people will be concerned about metadata on YouTube and what not.
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psycho_nauticus
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I just wipe my entire SAB with 70% iso and set everything in and wait for any air to settle and whatever alcohol to dry on the sides, and then I just work and run it dry... It's worked every time for me, and I believe sterile technique is the other most important key. If you have your sterile technique down then having to spray down your SAB, soapy water, wet towel at the bottom, etc. doesn't matter I find. Wipe down SAB with 70% isopropyl alcohol + sterile technique = works every time.
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Doctor Mario
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Quote:
psycho_nauticus said: I just wipe my entire SAB with 70% iso and set everything in and wait for any air to settle and whatever alcohol to dry on the sides, and then I just work and run it dry... It's worked every time for me, and I believe sterile technique is the other most important key. If you have your sterile technique down then having to spray down your SAB, soapy water, wet towel at the bottom, etc. doesn't matter I find. Wipe down SAB with 70% isopropyl alcohol + sterile technique = works every time.
Agreed. Im sure I'm screwing something up somewhere in the process. I've got some more prints on the way and then its back to the SAB but for now, sterile tek practice is on hold. Spawning 1 shoebox tomorrow and 5 more in the next few days. π
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Funky Monkey
Human Suppository


Registered: 05/14/19
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Quote:
Doctor Mario said:
Quote:
Funky Monkey said: This is a great idea for a general help thread for everyone!
A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.
Visual learners rejoice!
If you don't start it Mario, I will. Say the word. 
That was the intention of the thread but it never kicked off. A lot of people will be concerned about metadata on YouTube and what not.
Okay, well those people can chill in the background then lol
I just got out of the shower and my wheels were spinning the whole time with parameters for a thread like that to maximize the productive nature of it. I am going to write up a thread header for it with the guidelines/goals for the thread, and get a similar thread popping for the general cult community on here for all to participate in if they are of a mind to.
Thanks for posting this, it was a great start to the idea!
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Doctor Mario
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Thank you, sir. I'll be an avid poster in that thread for a while lol.
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Inthepit
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I'd be interested in participating if I knew how to strip meta data. I looked at YouT and it seems like it could be fairly anonymous.
I'm more eager to show off my hole in the SAB trick. Getting the wife to do a decent filming...uh well...
Edited by Inthepit (10/10/20 01:53 PM)
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Doctor Mario
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Re: Critique My Technique [Re: Inthepit]
#26978576 - 10/10/20 01:56 PM (3 years, 3 months ago) |
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I did a quick check on YTs metadata and couldn't find a straight answer. I'm not too concerned about it.
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MLPismyOPSEC
That One Ponyfucker


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Not to mention agar culturing and technique is not illegal, no matter the spores. Once active-species spores hit grain, THEN it's illegal I understand people would still think that having videos of agar could possibly/probably become probable cause, which is a fair point, but shit if some police officer is able to track me down based on a video or two and some posts here, they put in a shit load of work for basically zero payoff so they deserve to put me away.
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