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InvisibleDoctor Mario
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Re: Critique My Technique [Re: brad81684]
    #26963015 - 09/30/20 10:18 PM (3 years, 3 months ago)

More suggestions added to the list. :takingnotes:

Thank you.


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OfflineInthepit
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Re: Critique My Technique [Re: Doctor Mario] * 1
    #26963322 - 10/01/20 05:54 AM (3 years, 3 months ago)

Speaking of lists Doc here's mine...so far:

Washed 90 Qrt SAB, rack, and table, w/Clorox & towel
Books on top of SAB to anchor it down and reduce effects of bumping.
Nitrile gloves, mask, glasses, hat, iso gloves, (forearms soaped).
Wet towel under 3” high rack.
Lysol wipes under plates
iso the scalpel handle before
Hold scalpel toward the end
try flaming the entire blade all the way down to where it locks on.
Move slowly, don’t disturb the air
Lift petri dish cover very slowly
Move stack completely away from being over the bottom plate so fingertips aren't over the open plate.
don't bring the media bottle all the way back when pouring.
Don't bother cooling the blade, be the sizzle!
work at the back of the SAB


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: Inthepit]
    #26977304 - 10/09/20 04:44 PM (3 years, 3 months ago)

Just popping in here for an update on those plates I poured. 16 out of 20 grew mold, bacteria or both.


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InvisibleJHOVA
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Re: Critique My Technique [Re: Doctor Mario]
    #26977335 - 10/09/20 05:02 PM (3 years, 3 months ago)

Try no pour.


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: JHOVA]
    #26977339 - 10/09/20 05:06 PM (3 years, 3 months ago)

Thats my next plan of action. I enjoy pouring and really want to get it right.


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InvisibleJHOVA
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Re: Critique My Technique [Re: Doctor Mario]
    #26977352 - 10/09/20 05:16 PM (3 years, 3 months ago)

I recommend the holy grail containers. The cheaper ones for $20 on amazon are better bang for your buck.


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: JHOVA]
    #26977359 - 10/09/20 05:22 PM (3 years, 3 months ago)

Noted. Thank you, sir. On a more positive note, I came back today from a week long business trip and have some oat jars that I believe are 80-100% colonized.



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InvisibleNothingsChanged
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Re: Critique My Technique [Re: Doctor Mario]
    #26977887 - 10/10/20 02:38 AM (3 years, 3 months ago)

Something must be up, seems like a high contam rate?


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: NothingsChanged]
    #26978062 - 10/10/20 07:24 AM (3 years, 3 months ago)

Quote:

NothingsChanged said:
Something must be up, seems like a high contam rate?




I agree. This is the highest contam rate yet. Usually its only 2 or plates that contam from a pour session. Havent had that many go wrong since my first pours. Theres a lot of mold in this area. The dirt outside has a green hue and algae/mold grows on everything. Im sure we bring it in the house with is everything we go inside. Maybe I'm not moving slow enough in the SAB?

I dropped a wedge on accident and it fell on the wet towel. I grained it anyway and the jar went massive trich. Im gonna stop using the towel and see if things get any better.


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Offlinetryptkaloids
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Re: Critique My Technique [Re: Doctor Mario]
    #26978195 - 10/10/20 09:17 AM (3 years, 3 months ago)

Wash your carpets, scrub your sab. Pc your tools.


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: tryptkaloids]
    #26978418 - 10/10/20 11:49 AM (3 years, 3 months ago)

I clean my SAB before every use. No carpets, just hardwood floors and it probably wouldn't hurt to PC some tools.


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OfflineTheBoJim
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Re: Critique My Technique [Re: Doctor Mario]
    #26978463 - 10/10/20 12:32 PM (3 years, 3 months ago)

Quote:

Doctor Mario said:
Just popping in here for an update on those plates I poured. 16 out of 20 grew mold, bacteria or both.




I am about to start pouring my own for the first time. I have good luck with clones and transfers so far using premade plates. Lost only 3 to contam out for 40.

I don't use a towel or soapy water. Just the lid of the sab an a bunch of iso.

However lately I have been having trich issues in my tubs. I believe the cause is me doing G2G using quart jars to quart jars. Too big for my small SAB. I end up bumping the top of the box by the jar. Bigger box and/or smaller master jars are needed.


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InvisibleFunky Monkey
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Re: Critique My Technique [Re: Doctor Mario]
    #26978485 - 10/10/20 12:51 PM (3 years, 3 months ago)

This is a great idea for a general help thread for everyone!

A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.

Visual learners rejoice!

If you don't start it Mario, I will. Say the word. :rockon:


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: Funky Monkey]
    #26978507 - 10/10/20 01:08 PM (3 years, 3 months ago)

Quote:

Funky Monkey said:
This is a great idea for a general help thread for everyone!

A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.

Visual learners rejoice!

If you don't start it Mario, I will. Say the word. :rockon:




That was the intention of the thread but it never kicked off. A lot of people will be concerned about metadata on YouTube and what not.


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Offlinepsycho_nauticus
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Re: Critique My Technique [Re: Doctor Mario] * 1
    #26978517 - 10/10/20 01:12 PM (3 years, 3 months ago)

I just wipe my entire SAB with 70% iso and set everything in and wait for any air to settle and whatever alcohol to dry on the sides, and then I just work and run it dry... It's worked every time for me, and I believe sterile technique is the other most important key. If you have your sterile technique down then having to spray down your SAB, soapy water, wet towel at the bottom, etc. doesn't matter I find. Wipe down SAB with 70% isopropyl alcohol + sterile technique = works every time.


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: psycho_nauticus]
    #26978521 - 10/10/20 01:15 PM (3 years, 3 months ago)

Quote:

psycho_nauticus said:
I just wipe my entire SAB with 70% iso and set everything in and wait for any air to settle and whatever alcohol to dry on the sides, and then I just work and run it dry... It's worked every time for me, and I believe sterile technique is the other most important key. If you have your sterile technique down then having to spray down your SAB, soapy water, wet towel at the bottom, etc. doesn't matter I find. Wipe down SAB with 70% isopropyl alcohol + sterile technique = works every time.




Agreed. Im sure I'm screwing something up somewhere in the process. I've got some more prints on the way and then its back to the SAB but for now, sterile tek practice is on hold. Spawning 1 shoebox tomorrow and 5 more in the next few days. 😁


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InvisibleFunky Monkey
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Re: Critique My Technique [Re: Doctor Mario]
    #26978529 - 10/10/20 01:21 PM (3 years, 3 months ago)

Quote:

Doctor Mario said:
Quote:

Funky Monkey said:
This is a great idea for a general help thread for everyone!

A video based "Critique My Technique" thread where you specify that to request input/critiques on your xyz technique on this particular thread, you have to show a video (detailed pictorials can be okay, but nothing beats video). This way noobs and seasoned vets alike will get highly productive visuals, and highly productive input because people aren't guessing at any step of the process. I think this will be great for both posters, and the responders as it will be very engaging and informative in this format. I think it would be a powerhouse thread after a minute.

Visual learners rejoice!

If you don't start it Mario, I will. Say the word. :rockon:




That was the intention of the thread but it never kicked off. A lot of people will be concerned about metadata on YouTube and what not.




Okay, well those people can chill in the background then lol

I just got out of the shower and my wheels were spinning the whole time with parameters for a thread like that to maximize the productive nature of it. I am going to write up a thread header for it with the guidelines/goals for the thread, and get a similar thread popping for the general cult community on here for all to participate in if they are of a mind to.

Thanks for posting this, it was a great start to the idea!


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: Funky Monkey]
    #26978553 - 10/10/20 01:43 PM (3 years, 3 months ago)

Thank you, sir. I'll be an avid poster in that thread for a while lol.


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OfflineInthepit
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Re: Critique My Technique [Re: Funky Monkey]
    #26978568 - 10/10/20 01:51 PM (3 years, 3 months ago)

I'd be interested in participating if I knew how to strip meta data.
I looked at YouT and it seems like it could be fairly anonymous.

I'm more eager to show off my hole in the SAB trick.
Getting the wife to do a decent filming...uh well...:lol:


Edited by Inthepit (10/10/20 01:53 PM)


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InvisibleDoctor Mario
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Re: Critique My Technique [Re: Inthepit]
    #26978576 - 10/10/20 01:56 PM (3 years, 3 months ago)

I did a quick check on YTs metadata and couldn't find a straight answer. I'm not too concerned about it.


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