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OfflineGrenik
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Does Gentamicin need to be boiled?
    #26959278 - 09/28/20 06:39 PM (3 years, 4 months ago)

I want to make a few plates with Gentamicin sulfate for use when using a spore syringe or spore print to agar for the first time. After that, no Gentamicin in the agar.

I normally make a liter of agar at a time. Can I boil the agar without the Gentamicin, pour some of the agar into a smaller bottle with the Gentamicin in it, and then PC? Or does the Gentamicin need to be in the agar when it is boiled?

Thank you.


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OfflineOne of Us
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Re: Does Gentamicin need to be boiled? [Re: Grenik] * 1
    #26959426 - 09/28/20 08:41 PM (3 years, 4 months ago)

That should work fine. Give it a good shake before loading it in the pc. Gentamicin is pretty hygroscopic and dissolves in water easily. Especially at the concentration we use in agar.


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OfflineSpirit-Crusher
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Re: Does Gentamicin need to be boiled? [Re: Grenik]
    #26959462 - 09/28/20 09:18 PM (3 years, 4 months ago)

Add it to your agar mix. Bring to a boil. Sterilize. Gentamicin is used because its an antibiotic that will survive sterilization.


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OfflineGrenik
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Re: Does Gentamicin need to be boiled? [Re: One of Us]
    #26959490 - 09/28/20 09:48 PM (3 years, 4 months ago)

Quote:

One of Us said:
That should work fine. Give it a good shake before loading it in the pc. Gentamicin is pretty hygroscopic and dissolves in water easily. Especially at the concentration we use in agar.




Thank you, I will make a few plates the next time I pour.


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