Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Offlinesychevski
Stranger
Registered: 09/06/20
Posts: 4
Last seen: 8 months, 20 days
Possible contam?
    #26958442 - 09/28/20 08:19 AM (3 years, 4 months ago)

A friend of mine is experimenting with cubes in PF jars and in 2 inoculation point he noticed a strange discoloration. It started on day 4 after inoculation, first turning slightly off-white, and by day 5 getting noticeable shade of purple to it. I read up about possible contams, but I am puzzled by the fact it is happening in the very centre of growth and so early. White mycelium has been growing around the patch since without problems. My friend has isolated those 2 jars for now, just to be safe.

What could it be? Picture is as good as it gets with a phone camera.



Extras: Filter Print Post Top
OfflineSocrateshroom
сталкер


Registered: 09/05/18
Posts: 1,840
Loc: Westworld
Last seen: 1 month, 5 days
Re: Possible contam? [Re: sychevski]
    #26958539 - 09/28/20 09:39 AM (3 years, 4 months ago)

Interesting.

Looks like a slight pink discoloration and the mycelium at that spot looks "rubbery" from that picture.

I'm sure you read over the common contams thread:

https://www.shroomery.org/forums/showflat.php/Number/23130868/fpart/1/vc/1/nt/43

I'm not sure what it might be as I haven't personally run into many contams. I would mark it if I were you and monitor it, probably too hard to tell at the moment. Post pictures as/if it progresses.

Did you follow PF TEK when making these jars or did you make any modifications to the PF Tek recipe?

Or are these jars pre-made from a vendor?

Did you properly pressure cook or steam-sterilize the jars?

Did you use a SAB or flow-hood when inoculating?

And are there any other jars that are showing similar signs?


--------------------


Extras: Filter Print Post Top
Offlinesychevski
Stranger
Registered: 09/06/20
Posts: 4
Last seen: 8 months, 20 days
Re: Possible contam? [Re: Socrateshroom]
    #26958560 - 09/28/20 10:00 AM (3 years, 4 months ago)

PF-TEK from pdf guide posted somewhere in FAQ here, with a layer of dry verm on top of the substrate. Maybe the substrate was not mixed really well, so there are blobs of rice flour there, around one of which the pinkization happened. Jars were steamed for 2 hours covered with tinfoil. After inoculation tinfoil was on for 3 days, and then it was removed for better FAE. No flowhood or glovebox was used, but inoculation was done in a bathroom, all surfaces wiped with bleach, needle sterilized in flame fir each jar. My friend worn gloves, headwrap and a surgical mask. 2/8 jars have this pink spots in one of the inoculation points each. Cannot smell anything without opening the lid other than iodine or metal odor, which comes from all jars equally.


Extras: Filter Print Post Top
OfflineSocrateshroom
сталкер


Registered: 09/05/18
Posts: 1,840
Loc: Westworld
Last seen: 1 month, 5 days
Re: Possible contam? [Re: sychevski]
    #26958609 - 09/28/20 10:45 AM (3 years, 4 months ago)

Quote:

sychevski said:
PF-TEK from pdf guide posted somewhere in FAQ here, with a layer of dry verm on top of the substrate. Maybe the substrate was not mixed really well, so there are blobs of rice flour there, around one of which the pinkization happened. Jars were steamed for 2 hours covered with tinfoil. After inoculation tinfoil was on for 3 days, and then it was removed for better FAE. No flowhood or glovebox was used, but inoculation was done in a bathroom, all surfaces wiped with bleach, needle sterilized in flame fir each jar. My friend worn gloves, headwrap and a surgical mask. 2/8 jars have this pink spots in one of the inoculation points each. Cannot smell anything without opening the lid other than iodine or metal odor, which comes from all jars equally.




You'll have to keep an eye on it and the rest of the jars. If all or a majority of the jars have this contaminant (if it is indeed that), either your syringe was contaminated or your sterile technique was off.

Please note a few things:

At the very least a SAB (still air box) is very important for inoculation and I would highly recommend making one. Unfortunately the bathroom is not some magically sterile land and if inoculating in the open, there is a much better chance that contaminants are floating around and can land on the needle even after you've flamed it. A SAB will reduce those chances by making the air inside it "still" so there's less of a chance for contams to be flying around.

Did you remove the tinfoil, inoculate and then put that same foil back over the jar? That piece of tin foil, being reapplied, could have introduced contaminants into the jar (and if the dry verm layer failed, the contaminant might have gotten through to the spawn).

Tinfoil should only be on during sterilization. It should be removed when inoculating and should not be reapplied. They want the gas exchange right away. I don't know if preventing that early gas exchange can lead to any issues, a more knowledgable person would need to chime in.


At this point, the most you can do is wait and closely observe as it's growing. Keep an eye on your jars (but don't pick them up or you can disturb the dry verm layer and contaminants can fall into the colonizing myc).


--------------------


Extras: Filter Print Post Top
Offlinesychevski
Stranger
Registered: 09/06/20
Posts: 4
Last seen: 8 months, 20 days
Re: Possible contam? [Re: Socrateshroom]
    #26958749 - 09/28/20 12:30 PM (3 years, 4 months ago)

Quote:

Socrateshroom said:
Quote:

sychevski said:
PF-TEK from pdf guide posted somewhere in FAQ here, with a layer of dry verm on top of the substrate. Maybe the substrate was not mixed really well, so there are blobs of rice flour there, around one of which the pinkization happened. Jars were steamed for 2 hours covered with tinfoil. After inoculation tinfoil was on for 3 days, and then it was removed for better FAE. No flowhood or glovebox was used, but inoculation was done in a bathroom, all surfaces wiped with bleach, needle sterilized in flame fir each jar. My friend worn gloves, headwrap and a surgical mask. 2/8 jars have this pink spots in one of the inoculation points each. Cannot smell anything without opening the lid other than iodine or metal odor, which comes from all jars equally.




You'll have to keep an eye on it and the rest of the jars. If all or a majority of the jars have this contaminant (if it is indeed that), either your syringe was contaminated or your sterile technique was off.

Please note a few things:

At the very least a SAB (still air box) is very important for inoculation and I would highly recommend making one. Unfortunately the bathroom is not some magically sterile land and if inoculating in the open, there is a much better chance that contaminants are floating around and can land on the needle even after you've flamed it. A SAB will reduce those chances by making the air inside it "still" so there's less of a chance for contams to be flying around.

Did you remove the tinfoil, inoculate and then put that same foil back over the jar? That piece of tin foil, being reapplied, could have introduced contaminants into the jar (and if the dry verm layer failed, the contaminant might have gotten through to the spawn).

Tinfoil should only be on during sterilization. It should be removed when inoculating and should not be reapplied. They want the gas exchange right away. I don't know if preventing that early gas exchange can lead to any issues, a more knowledgable person would need to chime in.


At this point, the most you can do is wait and closely observe as it's growing. Keep an eye on your jars (but don't pick them up or you can disturb the dry verm layer and contaminants can fall into the colonizing myc).




I don't think the tinfoil caps prevented gas exchange, as for the first 3 days there wasn't much aerobic life in those jars anyway. And sterilized tinfoil exposed to an air for 10 seconds is still on the cleaner side of the universe.

6 jars look healthy to a noob's eye. Mycelium started to grow on day 3-4 and progresses slowly and evenly in all jars. Just these 2 show pink patches. Actually, the OP-jar was picked up every day to check on it and take pictures, so maybe ir caused something to fall through the dry verm layer.

If this grow fails my friend promises to think about SAB.

Thank you for the advice. I'll post an update as it developes.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Capsules   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Reduce contams with jars- extra step.
( 1 2 all )
aenima 5,668 22 05/25/02 02:49 PM
by groovysunrise
* Bright red/purple inky contamination oO_wombat_Oo 2,736 3 04/05/03 01:13 PM
by MAIA
* Bacterial Contam in MEA upupup 2,719 7 11/23/02 11:38 AM
by Seuss
* Need Some help + my Dream glovebox NewbieShroomer 4,039 9 02/26/14 07:44 PM
by shaymisco
* Reusing Contaminated Jars? NFG_Getupkid 10,731 19 06/26/02 05:18 PM
by bassplayer74
* Question on air exchange....
( 1 2 all )
Anonymous 5,350 21 04/28/11 09:01 AM
by RogerRabbit
* Contamination setbacks.. ShadowTH 1,352 10 06/19/02 08:51 AM
by ShadowTH
* Casing a contaminated cake!! Sporaticus 2,119 8 03/11/02 09:56 PM
by jonnyshaggs420

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
106 topic views. 37 members, 220 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.017 seconds spending 0.005 seconds on 12 queries.