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InvisibleNecroMyce
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I WANT TO MIX TWO STRAINS SPAWN
    #26958120 - 09/27/20 10:50 PM (3 years, 3 months ago)

So, I tried to look it up, but i could not find anything.
I was wondering if you knock up two jars, say... one with strain 1, and one with strain 2. Then prepare some grains and mix both strain 1 and strain 2 into the same grain back with G2G, would it grow together or compete? would the fruiting be a hybrid or would it be both strains? bad idea? good idea? thought it might be a cool experiment. Anyone have any links to pictures or posts of this being done before? I assume they would compete slightly and it wouldn't be worth it unless you have extra spawn.


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OfflinePretendhole
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: NecroMyce]
    #26958387 - 09/28/20 06:46 AM (3 years, 3 months ago)

They would not hybridize at a dna level. They would compete. It would be waste of spawn of one the strains


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InvisibleRoger Clemency
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Pretendhole] * 1
    #26958393 - 09/28/20 07:01 AM (3 years, 3 months ago)

You can actually cross that way, or just swab two separate varieties and put that to agar.

A good read if you’re wanting to try some yourself - https://www.shroomery.org/forums/showflat.php/Number/26254384/fpart/1/vc/1


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InvisiblebodhisattaMDiscordReddit
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Pretendhole] * 1
    #26958462 - 09/28/20 08:37 AM (3 years, 3 months ago)

Quote:

Pretendhole said:
They would not hybridize at a dna level. They would compete. It would be waste of spawn of one the strains



No they don't. Please don't talk out of your ass.

Every two spores that mate make a strain. You have thousands of strains in a grow if you start from spores. Spores don't care if a different syringe has a different name. Strains from different varieties get along just fine. Just like strains from the same variety get along.


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OfflinePretendhole
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: bodhisatta]
    #26958544 - 09/28/20 09:44 AM (3 years, 3 months ago)

Well slap me around and call me susan. Good info


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Offline3some
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: bodhisatta]
    #26958565 - 09/28/20 10:04 AM (3 years, 3 months ago)

I came across this: https://www.shroomery.org/forums/showflat.php/Number/7223720#7223720

and this:

https://www.shroomery.org/forums/showflat.php/Number/10242476#10242476

So my guess is, people select a fruit that exhibits a desirable trait (big cap? fat stipe?), clone it, fruit it, select and repeat for more than 6 generations to produce a stable variety.

So when you mix two varieties (say a big cap and a fat stipe), the different strains from both varieties will fuse and mix its genetic materials, producing fruits that could turn out to be anything, but greatly reducing the chances of fruits with big caps or fat stipes. In other words, pressing the reset button.

Close enough guess?


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OfflinePretendhole
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: 3some]
    #26958603 - 09/28/20 10:38 AM (3 years, 3 months ago)

Id like to try p.e and azurescens


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OfflineGrowtech
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Pretendhole]
    #26958707 - 09/28/20 12:00 PM (3 years, 3 months ago)

Quote:

Pretendhole said:
Id like to try p.e and azurescens



Good luck with that.  "Breeding" different species of mushrooms will work as well as breeding different species of animals (like a cat and a dog).

Cubensis and Azurescens are different species.  Now take two things within the same species (like Penis Envy and Golden Teachers... both Cubensis), you could have some success.  That's akin to breeding a poodle and doberman (both are dogs).


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Offline3some
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Pretendhole]
    #26958714 - 09/28/20 12:04 PM (3 years, 3 months ago)

Quote:

Pretendhole said:
Id like to try p.e and azurescens




Penis Envy is a variety of Psilocybe cubensis and a different species from Psilocybe azurescens. Individual from different species can't reproduce generally. Even if it is possible, it will not be biologically viable.


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Ask me about growing yeast, mold and bacteria. I'm very experienced. :tongue2:

The first post any newbie should read even before acquiring spores: Pastywhyte's Principles of mushroom growing for beginners


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InvisibleNecroMyce
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: NecroMyce]
    #26959450 - 09/28/20 09:05 PM (3 years, 3 months ago)

Honestly very good feedback here. Thank you all. It does make sense though that when you knock up from spores you don't have one specific set of genetics, you have tons of different genetics because you have different spores, and each spore is basically a genetic of its own correct? so if you select to spawn say, Penis Envy, your strain is penis envy, with spore inoculation, you have multiple genetics, thats why when you get to fruiting you have a multitude of different clusters, some bare spots etc, obviously you get variety in fruits for many different reasons, (casing or no casing, type of substrate, etc) but... it would be cool to stay withing the cubes and mix two strains that way, i wonder if you took Albino A+ with say golden teacher and got Golden Albino A +


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InvisibleRoger Clemency
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: NecroMyce]
    #26960101 - 09/29/20 10:47 AM (3 years, 3 months ago)

A “strain” is when two spores germinate and are compatible and the hyphae fuse to make dikaryotic mycelium (something along those lines, I don’t know all the exact wordage) so when you germinate spores you’ll have thousands of strains.

Psilocybe Cubensis is the species, things like APE, GT, B+ are different “varieties” but are really all the same deep down. Cubensis. Just like humans from Europe and Africa are the same, just with small variations based on where they came up. So any of those varieties can be crossed. APE is a cross between PE And PF albino. PE6 is a cross between a Texas variety and PE.

Someone on here I can’t remember who, he crossed TOC and PE and it’s a badass mix. Once you make these crosses though you have to stabilize it or it will revert back to one or the other. Something like that. The link I posted above is good info and also if you check out the saga of Rustywhyte that’s an interesting read. A cross of CRS and AA+ https://www.shroomery.org/forums/showflat.php/Number/25284782/fpart/1/vc/1


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Offlineseand04
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Roger Clemency]
    #26960192 - 09/29/20 11:47 AM (3 years, 3 months ago)

I plan on making a spore print of AA+, then placing a golden halo print right on top. Then to agar, see what happens.


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InvisibleNecroMyce
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Roger Clemency]
    #26969028 - 10/04/20 12:20 PM (3 years, 3 months ago)

Quote:

Roger Clemency said:
A “strain” is when two spores germinate and are compatible and the hyphae fuse to make dikaryotic mycelium (something along those lines, I don’t know all the exact wordage) so when you germinate spores you’ll have thousands of strains.

Psilocybe Cubensis is the species, things like APE, GT, B+ are different “varieties” but are really all the same deep down. Cubensis. Just like humans from Europe and Africa are the same, just with small variations based on where they came up. So any of those varieties can be crossed. APE is a cross between PE And PF albino. PE6 is a cross between a Texas variety and PE.

Someone on here I can’t remember who, he crossed TOC and PE and it’s a badass mix. Once you make these crosses though you have to stabilize it or it will revert back to one or the other. Something like that. The link I posted above is good info and also if you check out the saga of Rustywhyte that’s an interesting read. A cross of CRS and AA+ https://www.shroomery.org/forums/showflat.php/Number/25284782/fpart/1/vc/1




Super fascinating things. I knew the terminology basics, but this helped explain more. I feel like I should work on my Agar teks first, perfect that, and then go further before I mix and cross bread. I’m assuming to “stabilize” the crossbreed you would need to clone to agar, then sector it out and do transfers to you get a perfect rhizo circular growth.


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Offlineverum subsequentis
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Re: I WANT TO MIX TWO STRAINS SPAWN [Re: Pretendhole]
    #26969099 - 10/04/20 01:20 PM (3 years, 3 months ago)

Quote:

Pretendhole said:
Well slap me around and call me susan. Good info





:snapeslap:
Susan, Please refrain from speaking of that which you know nothing about. These boards are filled with enough bullshit as it is. A good rule of thumbs for noobs (in all subjects of life) is: "i will not speak out of my ass".


Edited by verum subsequentis (10/04/20 01:21 PM)


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