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OfflineSvampeB0b
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2 contaminated tubs in a row (HELP)
    #26955956 - 09/26/20 11:39 AM (3 years, 4 months ago)

Hi

I'm getting a little frustrated with my mono-tubs, and i'm not sure what i'm doing wrong :/ I've successfully grown a few tubs, but the last to contaminated even before pinning. In both instances all jars looked fine, so I think i'm doing something wrong when i put it into bulk.

My substrate is 1 brick coco coir, 2qt verm and 4qt boiling water into a bucket.

I wipe down my tub with water and then alcohol just to make sure. I wear gloves and are careful when mixing the substrate with the spawn.

My tubs are pretty simple, 6 holes with polyfill. I add the polyfill after I mix it, put the lid on and then I just let it sit. I wait about 10 days for it to pin, and then I start to do some FAE if needed, but the last 2 tubs have shown green contamination on the surface after about 7 days.

How can I optimize or change my process to avoid contamination?


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Offlinepolaritymind
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Re: 2 contaminated tubs in a row (HELP) [Re: SvampeB0b]
    #26956020 - 09/26/20 12:20 PM (3 years, 4 months ago)

Well thw big question is how you inoc your grain jars? Agar (and SAB?) or MS (Multispore) Syringe. If second, selfmade or bought.


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OfflineGan
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Re: 2 contaminated tubs in a row (HELP) [Re: polaritymind]
    #26956036 - 09/26/20 12:27 PM (3 years, 4 months ago)

Yeah so everything you mentioned in the OP will have very little effect on your contamination.

Generally, that starts and ends with your culture. Is it cleaned up on agar beforehand? Is it put to grain with good sterile technique? Is everything that needs to be sterile done so correctly and successfully? Those factors and any other that are related to the health/cleanliness of your spawn/culture will determine whether you will have success or not.

The way you prep your substrate, or wiping out your tubs, or when you introduce FAE, whether you wear gloves when spawning, etc. will have little to no bearing on the health of your tubs. So first order of business is to shift your focus and second is to explain your process


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OfflineSvampeB0b
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Re: 2 contaminated tubs in a row (HELP) [Re: Gan]
    #26956109 - 09/26/20 01:16 PM (3 years, 4 months ago)

That's good to know.

I use spore syringes that I got from webshop vendor.

I use rye berries that I rinse, add a tsp. gypsum, soak over night for about 18-24 hours.

I boil them for about 10 min. Let them sit to get the correct moister and then I add them to my jars. I clean my jars, wipe them down with alcohol. Same goes for the lids which are plastic lids with self healing injection ports and micropore tape. I put a piece of tape on the inside and outside. I cover the jars with tinfoil, put them into the pressure cooker and cook them for 90 min. I let them cool over night and then i'll move them into a SAB. I clean the SAB and spray the inside with water. I wipe down the lids / injection ports with alcohol (70%) - Flame sterilize the needle and inject about 2cc into each 1qt jar. Everything wearing gloves and cleaned with alcohol.

Are you guys saying that it's more or less impossible that the contamination comes from moving them to the tub? I would have to happen either from bad spores or me messing up the injection or sterilizing?


Edited by SvampeB0b (09/27/20 11:22 AM)


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OfflineGan
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Re: 2 contaminated tubs in a row (HELP) [Re: SvampeB0b]
    #26956408 - 09/26/20 04:52 PM (3 years, 4 months ago)

First off, remove the merchant's name from there post. They're a sponsor.

Second, if you're shooting spore solution straight to grain, it's always going to be a crapshoot. Spores are never sterile. So it's literally just a random chance of whether it will work or not.

Also, are you venting your PC for 10-15 minutes before putting the weight on and getting to 15 psi?

And yeah, based off what you've said I can just about guarantee your contam problems are not from anything to do with spawning or moving your tubs


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OfflineSvampeB0b
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Re: 2 contaminated tubs in a row (HELP) [Re: Gan]
    #26957315 - 09/27/20 11:25 AM (3 years, 3 months ago)

Thank you Gan

I removed the merchants name.

Is there less risk of contamination when doing agar vs spore syringes?


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Offlineshroomshine
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Re: 2 contaminated tubs in a row (HELP) [Re: SvampeB0b]
    #26957426 - 09/27/20 12:59 PM (3 years, 3 months ago)

absolutely 100% yes - you can eliminate pretty much all contaminations (bacterial and mold) by doing multiple transfers on agar


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OfflineSvampeB0b
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Re: 2 contaminated tubs in a row (HELP) [Re: shroomshine]
    #26960022 - 09/29/20 09:45 AM (3 years, 3 months ago)

Thank you guys!

Yes, I let the PC vent for 15 min before adding on the weight and I start the timer when it hits 15psi.

I think I'll start my agar adventure :smile:

I just PC'ed a new set of jars, and I noticed that my micropore tape was wet when I pulled out the jars, even though they were covered in tinfoil. Is that okay?

Also, I had to leave the jars in the PC due to work and they ended up sitting over 24 hours in the PC after cooking them. Is there any harm to that?


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Offlineshroomshine
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Re: 2 contaminated tubs in a row (HELP) [Re: SvampeB0b]
    #26960495 - 09/29/20 02:55 PM (3 years, 3 months ago)

regarding the wet MP tape... definitely not ideal but you "might" get away with it. how many layers do you use? I would do at least three (you can fold one piece in half, so there is no sticky side and then cover it with another one - helps with cleaning it later as well).
I would recommend unmodified plastic lids tho, just did my first round with those and I am absolutely hooked (search for pasty's tek)!
But if you want to stick with the tape, cover the lid with a folded (few folds) piece of paper towel before putting the foil on, that should help with getting it wet.

I let my jars cool outside the PC but I guess you should be good on that.

Good luck and have fun with your agar adventure!


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Edited by shroomshine (09/29/20 02:58 PM)


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OfflineSvampeB0b
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Re: 2 contaminated tubs in a row (HELP) [Re: shroomshine]
    #26963935 - 10/01/20 01:55 PM (3 years, 3 months ago)

I'll try and experiment a bit with it. The lid tek seems interesting.

I'm gonna dive into the agar world and try that out :smile:


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