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Bombi
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Blue ring on agar mycelium
#26955951 - 09/26/20 11:35 AM (3 years, 4 months ago) |
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Hey 
First time starting to grow (from a spore print) and now I struggle getting started. The mycelium in the picture (should) be psilocybe cubensis indo. So I got this agarplate and I have this blue ring now and that fuzzy looking mycelium. Since I am very new to this all I wanted to ask you if that's mold. I looked at the contamination thread but did not see a blue ring like that, so I am not sure. Thank you all in advance for the help!!

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Ep1429
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Re: Blue ring on agar mycelium [Re: Bombi]
#26955972 - 09/26/20 11:50 AM (3 years, 4 months ago) |
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Hold it up to a light, both sides. Like this:

Especially the bottom, I can’t really make anything out.
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Bombi
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Re: Blue ring on agar mycelium [Re: Ep1429]
#26956078 - 09/26/20 12:49 PM (3 years, 4 months ago) |
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the first one is from the front

the second one from behind.
Thanks already for the fast response!
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verum subsequentis
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Re: Blue ring on agar mycelium [Re: Bombi]
#26956113 - 09/26/20 01:17 PM (3 years, 4 months ago) |
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I got money on that being mold. Doesn't look like cube myc and looks a lot like mold.
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Bombi
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Quote:
verum subsequentis said: I got money on that being mold. Doesn't look like cube myc and looks a lot like mold.
FUCK!^^ Ok thanks, back to the beginning.
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verum subsequentis
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958190 - 09/28/20 12:31 AM (3 years, 3 months ago) |
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got any updated pics? A few days can tell a lot.
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Bombi
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Quote:
verum subsequentis said: got any updated pics? A few days can tell a lot.
it looks pretty much the same (just a bit worse^^). I post one later that day.
I made some new agar plates, let's see how they turn out. I already made two of other species which look pretty good. But they are very hard to grow indoor so these will be a project for a later time.
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Zenn
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958224 - 09/28/20 01:44 AM (3 years, 3 months ago) |
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Contamination.
The yellow one looks like bigger version of one I had before that was round yellow and shiny.
Generally if there is any colour other than white it's probably not a good thing. I had some trichoderma grow looking an awful lot like good myc to start with then its centre went green and started getting dusty. Also once had a thing like a tiny white snow ball that looked grey from the other side. It grew into like half a ball shape.
Sorry to say the pictures above dont look too positive bud :/
So good luck with your future attempts Also, what kind do you have that's difficult to do Indoors?
Edited by Zenn (09/28/20 01:47 AM)
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Bombi
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Re: Blue ring on agar mycelium [Re: Zenn]
#26958250 - 09/28/20 02:45 AM (3 years, 3 months ago) |
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Quote:
Zenn said: Also, what kind do you have that's difficult to do Indoors?
Thanks for your reply . I also thought that they are contaminated because the color seemed weird, but since I do not have anything going, I hoped and asked, because starting from the beginning takes some time 
I have Psilocybe cyanescens and psilocybe serbica as well. I can post the agar plates of these two if you are interested as well. But I probably won't grow them till I fruited some cubes successfully. The cube picture I will post later that day.
Edited by Bombi (09/28/20 02:46 AM)
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Zenn
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958268 - 09/28/20 03:31 AM (3 years, 3 months ago) |
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Yeah that would be cool to see them on plates. I do love a nice myc pic. Hahahaha starting from beginning does take some time! If you want to take a look here is my current project for indoor ps. Cyanescens, maybe some of the info can help you with ideas for yours? It has been taking quite some time but paying off. One thing you will need above all else for that project is patience
https://www.shroomery.org/forums/showflat.php/Number/26535887
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Bombi
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Re: Blue ring on agar mycelium [Re: Zenn]
#26958711 - 09/28/20 12:02 PM (3 years, 3 months ago) |
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So this is the contaminated one. Looks like the top right would be a good mycelium? But of course I will throw it away.

this one is the cyanscens

this one is the serbica
The other two are looking pretty good to me, but then again, it's my first try, so maybe they are contamed as well :/
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verum subsequentis
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958721 - 09/28/20 12:11 PM (3 years, 3 months ago) |
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The last two are looking good. The top one looks like it may be cool in that one spot. No need to toss her. That's exactly what SABs are good for. If you have your sterile tek on point, you can open her up and grab a piece of that good growth, transfer it to a new plate and leave the shit stain undisturbed.
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Bombi
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hasn't the big one spread it's spores? Wouldn't it be safer to toss it?
where would you transfer it to? Liquid Culture or another agar plate?
Edited by Bombi (09/28/20 01:11 PM)
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verum subsequentis
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958816 - 09/28/20 01:19 PM (3 years, 3 months ago) |
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Never ever ever ever ever transfer a questionable something to LC. Always transfer to fresh plates until you have a clean plate. Then proceed with whatever expansion tek you like.
The air inside a petri dish is still and the spores remain held to the surface by what we like to call gravity. That is unless you've been shaking it up.
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Bombi
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Quote:
verum subsequentis said: Never ever ever ever ever transfer a questionable something to LC. Always transfer to fresh plates until you have a clean plate. Then proceed with whatever expansion tek you like.
The air inside a petri dish is still and the spores remain held to the surface by what we like to call gravity. That is unless you've been shaking it up.
Ok, that's what I thought. Maybe I shouldn't question myself so much but I am kinda scared losing the little progress I made xD.
Would you transfer it right now, or let it grow a bit more?
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verum subsequentis
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Re: Blue ring on agar mycelium [Re: Bombi]
#26958827 - 09/28/20 01:27 PM (3 years, 3 months ago) |
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I'd transfer it whenever i had a free moment. Doesn't really matter. Now is fine. Tomorrow is fine. The next day is fine. Wouldn't wait too long though.
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Zenn
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Yeah the bottom 2 do look good 
If you want to try save the good looking little bit from the other one, sooner better than later etc, like they said above
Do hope you manage
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