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OfflineSN122A
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Registered: 10/09/09
Posts: 223
Loc: Flag
Last seen: 1 year, 1 month
White mould?
    #26955595 - 09/26/20 05:44 AM (3 years, 4 months ago)

Might be a silly question]but what's the chances of a contaminate mould in cultures (agar) that can't be easily distinguished from myc but then shows blue-green after a month or so?

I've had a lot of troubles getting prints so reluctantly got a few syringes.  Vendor was not ideal but hardly anyone seems to have stock and fewer still will do anything outside the USA at the moment. (At least I can get a lot of the edibles in Aus these days which is my primary focus)

Anyway, germination->p3 everything looks ok...  I keep a few plates of the same I used for inoculation so I can go back to the same plate if results are good.

Fast forward few dozen bags, bottles, months etc and I seem to have what looks like a mould turn up.  Thing is I kept growing out dishes etc without obvious visible issue.  Now those first reference dishes are 3 months old and BAM a weird ring of green...



Also this weird overlay type growth, it didn't look like that a mouth ago.  Possible contaminate overthrowing the original culture?

So this was theoretically pan cyan, although I did 30 plates and got only 2 germinate anything so quality was rubbish as far as spore count but clean.  Unfortunately both were more linear type growth, less wispy but figured if I get a few caps I could select better genetics.

(Ignore middle color, I was experimenting with activated carbon agar on exotics and used a few leftover plates, hence black colour)


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Offlinepolaritymind
relaxed attention
I'm a teapot

Registered: 10/10/16
Posts: 994
Loc: Germany Flag
Last seen: 3 months, 8 days
Re: White mould? [Re: SN122A]
    #26955659 - 09/26/20 07:06 AM (3 years, 4 months ago)

Yes, strange that it took so long but that is certainly a dirty plate. Not just the Green but also the "mycelium" looks off, its too thick "blobby". And unstrucured, no hyphae (strands of mycelium) visible.

It could be due to faulty sealing/filter, that a contan got in after proper colonization. Do you have any pictures from before, how the plates looked when they were covered with mycelial growth?


--------------------
"to affirm life is to also affirm death"
-Albert hofmann


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OfflineSN122A
Tweaker


Registered: 10/09/09
Posts: 223
Loc: Flag
Last seen: 1 year, 1 month
Re: White mould? [Re: polaritymind]
    #26956667 - 09/26/20 08:34 PM (3 years, 3 months ago)

No pics earlier unfortunately. I didn't take any because nothing looked particularly interesting.

It also seems to have later grown that zonate style bubbly form. Initially it looked like pretty plain cotton myc.

It had sectors of mostly linear cottony type growth (too thick for good pan usually but all I got from that crappy syringe).

What's weird is this covered the plate and stayed white since July.  I try to often keep a master plate in the fridge, even when I expect/ presume the culture is probably ordinary.

But yeah had it out of the fridge a few weeks after investigating the origin of presumed dirty spawn then after a few warm days got that green ring...

I guess I usually seen tric type mould that was colored colonies from early on and white satellites with a structure that was visually different from expected myc.  First time a had something seem to masquerade a few months like a ninja in ambush lol.


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OfflineSN122A
Tweaker


Registered: 10/09/09
Posts: 223
Loc: Flag
Last seen: 1 year, 1 month
Re: White mould? [Re: SN122A]
    #26956670 - 09/26/20 08:36 PM (3 years, 3 months ago)

I guess what might not be clear in that pic is the thin myc going all the way to the plate edge (it was all like that until recently.


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