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OfflinePinkAlpha
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Registered: 04/27/20
Posts: 13
Last seen: 2 years, 6 months
Can you sterlize an agar jar on its side in a PC?
    #26954917 - 09/25/20 06:38 PM (3 years, 4 months ago)

Ok minor emergency, I'm just geting started, I bang my 500ml pyrex media bottle w/ MEA agar into my pressure cooker...and the lid doens't go on...the bottles too big.

I'm a dumbass I know, I know I shouldn't have bought a pressure cooker that was only 8L, but the past is in the past.

However, if I tip my media bottle on its side, and kinda just let it...float in the water in the pressure cooker, it can fit. Only problem is i'd have to have the lid on real tight, instead of loose.

Can I do this? Will my glass media bottle explode? Advice really appreciated cause I'm all set up here and I'm stalled.


Edited by PinkAlpha (09/25/20 06:47 PM)


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OfflineEp1429
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Registered: 10/10/18
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Re: Can you sterlize an agar jar on its side in a PC? [Re: PinkAlpha]
    #26954926 - 09/25/20 06:54 PM (3 years, 4 months ago)

It will not explode. The pressure outside the glass is going to be 15 PSI, or higher.  Regular canning is sealed.

It might leak.


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OfflinePinkAlpha
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Re: Can you sterlize an agar jar on its side in a PC? [Re: Ep1429]
    #26954938 - 09/25/20 07:01 PM (3 years, 4 months ago)

Its a GL45 screw cap, so i don't think it's likely to leak.
You think its worth giving it a shot?


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OfflineEp1429
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Re: Can you sterlize an agar jar on its side in a PC? [Re: PinkAlpha]
    #26954951 - 09/25/20 07:11 PM (3 years, 4 months ago)

Quote:

PinkAlpha said:
Its a GL45 screw cap, so i don't think it's likely to leak.
You think its worth giving it a shot?




Ab-so-f*ckin-lutely!  It’s not my PC.

J/k.  I have the same media bottle, and mine leaks if I don’t screw the cap down, then back it off a single 1/4 turn, so all of my agar runs are in a sealed jar.

You ever seen a jar of canned tomatoes?  If the liquid in there doesn’t cause it to explode at pressure, the agar won’t.


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OfflinePinkAlpha
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Re: Can you sterlize an agar jar on its side in a PC? [Re: Ep1429]
    #26954994 - 09/25/20 07:32 PM (3 years, 4 months ago)

I'm sorry if I'm being obtuse or slow...but when you say absoloutely, its not my pressure cooker, then jk...it sounds like you're telling me I'm going to fuck my pressure cooker, or my something up.

EDIT: Aight well I did it and it worked. Thanks anyway.


Edited by PinkAlpha (09/25/20 08:08 PM)


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InvisibleSir Pentinite
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Registered: 05/15/19
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Re: Can you sterlize an agar jar on its side in a PC? [Re: PinkAlpha]
    #26955184 - 09/25/20 10:15 PM (3 years, 4 months ago)

I do 500mL media bottles leaned-over in a smaller PC than yours. I use empty narrow-mouth half-pint jars to keep it from falling over. Just keep the agar as far from the bottle's top as you can and fill the PC water up pretty high to mitigate boilover.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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OfflineEp1429
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Re: Can you sterlize an agar jar on its side in a PC? [Re: PinkAlpha]
    #26955213 - 09/25/20 10:44 PM (3 years, 4 months ago)

Quote:

PinkAlpha said:
I'm sorry if I'm being obtuse or slow...but when you say absoloutely, its not my pressure cooker, then jk...it sounds like you're telling me I'm going to fuck my pressure cooker, or my something up.

EDIT: Aight well I did it and it worked. Thanks anyway.




Told ya.  Canned foods are sealed tight and go through a PC cycle just fine.


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InvisibleSir Pentinite
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Re: Can you sterlize an agar jar on its side in a PC? [Re: Ep1429]
    #26955246 - 09/25/20 11:11 PM (3 years, 4 months ago)

Perhaps, but regular media bottles are not rated for pressure and only specific ones are rated for vacuum.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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Invisiblehobbyguy
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Registered: 05/11/08
Posts: 119
Re: Can you sterlize an agar jar on its side in a PC? [Re: Sir Pentinite]
    #26955691 - 09/26/20 07:45 AM (3 years, 4 months ago)

I use jar lids in the bottom of my small PC and lean the media bottle over. Jar lids keep it from moving. Keep cap loose and use foil over it. Don't lay it on it's side


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OfflineEp1429
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Re: Can you sterlize an agar jar on its side in a PC? [Re: Sir Pentinite]
    #26955940 - 09/26/20 11:27 AM (3 years, 4 months ago)

Quote:

Sir Pentinite said:
Perhaps, but regular media bottles are not rated for pressure and only specific ones are rated for vacuum.




They can be run through an autoclave.  Anything that can handle that can handle our stove top PCs.


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OfflineEp1429
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Re: Can you sterlize an agar jar on its side in a PC? [Re: hobbyguy]
    #26955941 - 09/26/20 11:28 AM (3 years, 4 months ago)

Quote:

hobbyguy said:
I use jar lids in the bottom of my small PC and lean the media bottle over. Jar lids keep it from moving. Keep cap loose and use foil over it. Don't lay it on it's side




I put mine in the center of the rack and wrap a dish towel around it’s base.


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