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OfflineWallabymince
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Possible contams on MEA and rapid growth on unknown species - noob
    #26954896 - 09/25/20 06:23 PM (3 years, 4 months ago)

Hello guys,

Im new here but have done some reading so my set up uses a presto 23qt PC, SAB and MEA agar. This is my first attempt.

Im having two problems

One is that I got two syringes from a vendor and one may not be cubensis the vendor couldn't tell me whether they are or not. I am hoping they are mexicana (for sclerotia). The name it was given is "teonanactl". Maybe someone knows what they are i will fruit them regardless and leave a jar or two to see if stones form.

But the real problem

I hotpoured sterile agar to 15 plates in my SAB. inoculated 5 with the previously mentioned spores from a syringe. inoculated 10 from another syringe of GT's. Within two days the unknown one has growth all over all 5 plates(Spore liquid went all over the plate, lol). The GT's however appear to have slime covering all the plates and no myc. It has only been 4 days. I cant tell if the slime is from excess liquid and agar that condensed like that or if theres some contam.

Theres none of this on the 5 other plates and they were all poured at the same time. I had tried to use the GT to inoculate jars earlier so maybe I contammed the syringe. But its early for GT to have myc so I want to wait but im not trying to farm bacteria over here.

I can attach pics but if its obviously bacteria let me know or maybe the excess liquid after inoculation would appear slimy on top of the agar like this?

Also i inoculated 7 jars 4 GT and 3 of teo, its all been inncubating at 23°C-30°C and getting 12hrs light.

Ps. I know the spores are everywhere on the T plates, very sloppy, please dont flame me the syringe would squirt a ton of liquid everytime i touched it lol. I plan to use agar or prints after i get some fruit.



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OfflineOne of Us
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Wallabymince]
    #26954904 - 09/25/20 06:30 PM (3 years, 4 months ago)

Yep, that's bacteria.


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OfflineCocaineBuffet
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: One of Us]
    #26954980 - 09/25/20 07:23 PM (3 years, 4 months ago)

Spore syringes depending on the vendor can range from clean to filthy. I would recommend you put a single drop from the syringe on the agar and then zig-zag it across the agar surface with either an inoculation loop (heated to red hot) or use a sterilized q-tip.

When first getting into agar one of the mistakes most people make (myself included) is to put too much solution onto the plate. It only takes a tiny amount of spores to begin to create mycelium. You will likely need to transfer a few times before it is healthy enough to inoculate grain but that is why the Agar Envy thread is so helpful. :thumbup:

Share your pictures in that thread and people will be ready to help you identify what is mold or where you should transfer from.


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OfflineWallabymince
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: One of Us]
    #26955435 - 09/26/20 02:01 AM (3 years, 4 months ago)

The presence of it on the 10 GT plates and not the unkown ones would suggest it is in the syringe then, wouldnt it?

Which means i only have the unidentified strain/variety to work with as i only kept like 12ml of the GT syringe and i dont see any way to seperate it from the bacteria. Should i wait to see if myc speouts for transfers or toss?



Edited by Wallabymince (09/26/20 06:58 PM)


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OfflineWallabymince
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: CocaineBuffet]
    #26955437 - 09/26/20 02:03 AM (3 years, 4 months ago)

Can I just post pictures in the agar envy thread and wait for a reply?


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OfflineOne of Us
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Wallabymince]
    #26957116 - 09/27/20 07:44 AM (3 years, 3 months ago)

Yeah, it doesn't look like you'll be able to get anything usable from that GT syringe. Maybe if you used antibacterial agar. But I would focus on the unknown strain which looks pretty clean ( still needs some transfers ).

Of course you can post those pictures in the agar envy thread. Lots of helpful people there


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OfflineWallabymince
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: One of Us]
    #26957267 - 09/27/20 10:29 AM (3 years, 3 months ago)

Thankyou for the help :smile:

I can live with that especially seeing how vigorous this other one is


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InvisibleRoger Clemency
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Wallabymince]
    #26957418 - 09/27/20 12:52 PM (3 years, 3 months ago)

A lot of spore syringes will be bacterial I’ve noticed. It’s much better to get spore prints for germinating on agar.

With your bacterial syringe you can try to put it straight to grain, or to a little BRF puck in a small jar, and once it terminates you’d take a colonized grain/piece of the BRF and put that on an agar plate to clean it up from there.

Teonanactl should indeed be P Mexicana unless someone just wanted to put a cool sounding old name on a cube variety.


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OfflineWallabymince
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Roger Clemency]
    #26957538 - 09/27/20 02:22 PM (3 years, 3 months ago)

Thankyou for the reply, you echoed the conclusions I reached about trying to get some GT's going

I also figured that would be the case with teo from an old post about mexicana that used that name (also wikipedia) either way its vigourous as myc, can you fruit mexicana with as many fruit as cubensis gram wise?


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InvisibleRoger Clemency
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Wallabymince]
    #26957870 - 09/27/20 06:41 PM (3 years, 3 months ago)

I’m not sure what you mean with there. I’ve grown tampenensis and atl7 which I think is just mexicana or related or something. They’re about 1.5x as strong as average cubes.

If you mean the dry weight per grow that all depends on genetics. Luck or how much work you put in finding a culture. But generally they’re a little tougher to fruit and yield less than cubes. You should have plenty of stones in the sub though.


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OfflineWallabymince
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Re: Possible contams on MEA and rapid growth on unknown species - noob [Re: Roger Clemency]
    #26958260 - 09/28/20 03:12 AM (3 years, 3 months ago)

Okay thanks for the info,

I originally requseted atl7 because I am planning on trying get some stones from them.

With the bad GT though I hope i can still get a full flush on bulk substrate with the mexicanas cause i want plenty of fruitbodies.

What would you suggest is the best strain of cubensis for yield?


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