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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: Funky Monkey]
#26978823 - 10/10/20 05:02 PM (3 years, 3 months ago) |
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Interesting I saw this about the food coloring thing. I have long suspected I had problems if I crossed a certain threshold of the pink McCormick I have from one of their "neon" four packs (pretty sure that's what it's called). Added a little more than usual last time by accident and suddenly I have 20 dishes (4 species and multiple varieties that were looking just fine before). Same everything save for the coloring and voila: seriously slow and weak growth across the board. Only ones that came out looking ok was my cinctulus dishes and even they look weak AF compared to what they were... Been using mostly blue with zero problems and I believe I recall getting fucked by this AND magenta before to some degree. I think that it just takes a little too much and you go from ok to not so much. Never ever had this problem with blue or black.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: alaskappalachian]
#26978827 - 10/10/20 05:05 PM (3 years, 3 months ago) |
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Well fuck it then, no more red. I like blue better, but I used to like mixing purple.
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starbones
I'm an alien, I eat uranium.



Registered: 03/04/20
Posts: 1,131
Last seen: 2 months, 17 days
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: Funky Monkey]
#26978830 - 10/10/20 05:07 PM (3 years, 3 months ago) |
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Quote:
Funky Monkey said:
Quote:
starbones said: Someone had parents that failed at raising their child right.
The replacement gasket kit I bought locally from the hardware store had a used gasket in it I found when I went to replace mine today. No more stock at the store and mine is leaky. I doubt Grandma Mable would do this shit so I have to assume it's some fellow local cultivator who wanted to save twenty dollars.
Loser.
Where are you located? Also, was it walmart?
Canada and no it was a Home Hardware, I debated about going back with my original gasket and the one that was in the box but in the end I'll take a $20 loss instead of looking like the guy trying to pull a fast one on customer service. They have enough to worry about in a day.
I'm really hoping that All American gets their stock issues straightened out soon or I'm heading to Alibaba and looking for a large autoclave.
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: starbones]
#26978840 - 10/10/20 05:10 PM (3 years, 3 months ago) |
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I feel you.
I'm waiting on my 75x as we speak, luckily they aren't having as bad of supply issues with those bigger expensive plug-ins.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: Funky Monkey]
#26979785 - 10/11/20 10:23 AM (3 years, 3 months ago) |
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Leave your half gallon masters outdoors for a year tek
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Funky Monkey
Human Suppository


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: Munchauzen]
#26979792 - 10/11/20 10:25 AM (3 years, 3 months ago) |
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When can we expect the grow log???
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CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 3,455
Last seen: 18 hours, 35 minutes
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: Funky Monkey] 2
#26979879 - 10/11/20 11:41 AM (3 years, 3 months ago) |
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nogoodnamesleft
Stranger than average


Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 13 days, 21 hours
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: CocaineBuffet]
#26980661 - 10/11/20 09:59 PM (3 years, 3 months ago) |
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First night working with my flow hood in its non permanent location and I took the agar media bottle out of laminar flow to put back in the water bath uncapped!! Luckily I have another sterilized one I made at the same time as the one I fucked up.
I’m melting that one now.
I also put my gloved hands over plate that were cooling without iso spraying them before going back in the flow hood.
So I fucked up there too.
Luckily I’m catching all these mistakes as I make them and getting laughed at by my wife and laughing at myself too.
Sterile technique is easy. 90% of it is common sense 10% is science ( using correct sanitizing compounds ) IMO...
I’m just so excited to be doing something in this thing that I guess I wasn’t being mindful. That’s probably sterile tech rule number 1 ... slow the fuck down and think out what you’re doing
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: nogoodnamesleft]
#26980663 - 10/11/20 10:03 PM (3 years, 3 months ago) |
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The gloves you’re probably fine. I only do mine once unless I’m moving around touching lots of things like my phone or my margaritas. As long as you’re not touching scalpel tips or the agar you’re probably fine. The air flow should be blowing anything that falls off you away from your work.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 7 hours
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: D3_Myc]
#26980673 - 10/11/20 10:21 PM (3 years, 3 months ago) |
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When you work in the flow do the same principles apply as a sub, just foreward?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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nogoodnamesleft
Stranger than average


Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 13 days, 21 hours
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: D3_Myc]
#26980675 - 10/11/20 10:26 PM (3 years, 3 months ago) |
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Quote:
D3monic said: The gloves you’re probably fine. I only do mine once unless I’m moving around touching lots of things like my phone or my margaritas. As long as you’re not touching scalpel tips or the agar you’re probably fine. The air flow should be blowing anything that falls off you away from your work.
Now I know for next time. I took those plates out of the laminar flow without wrapping them in Saran Wrap ... Parafilm arrives next week.
I’m about to order 2500 sterile dishes.
How thick do y’all pour the dishes
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: nogoodnamesleft]
#26980678 - 10/11/20 10:28 PM (3 years, 3 months ago) |
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hopefully they are all still sterile when you go to use them
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: TedsDead]
#26980702 - 10/11/20 10:57 PM (3 years, 3 months ago) |
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I slide mine back into the sleeve they came in. Much easier than wrapping individual dishes.
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nogoodnamesleft
Stranger than average


Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 13 days, 21 hours
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: TedsDead]
#26980707 - 10/11/20 11:04 PM (3 years, 3 months ago) |
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Quote:
TedsDead said: hopefully they are all still sterile when you go to use them
Because I’m a whole noob I just tossed the plates from the debacle that I described in my fail post.
Just melted some regular LMEA and some supplemented with carbon. I’m going to pour 9 plates of each.
I have a bunch of holy grail plates to try no pour agar
Do I even need to boil it with no pour? Or even if I use media bottles?
Seems like if it’s going in the PC the ramp up to pressure will boil the contents of the bottle or the plates
Help me out with my logic here
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: nogoodnamesleft]
#26980733 - 10/11/20 11:34 PM (3 years, 3 months ago) |
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With no pour I recommend a boil so that you can evenly distribute the agar and malt extract. I havent tried the holy grail plates yet but it shouldnt boil it out.
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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nogoodnamesleft
Stranger than average


Registered: 09/19/20
Posts: 413
Loc: Melmak
Last seen: 13 days, 21 hours
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: TedsDead]
#26980773 - 10/12/20 12:22 AM (3 years, 3 months ago) |
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Quote:
TedsDead said: With no pour I recommend a boil so that you can evenly distribute the agar and malt extract. I havent tried the holy grail plates yet but it shouldnt boil it out.
Ive got 50 of them that I’m going to prep tomorrow.
I printed Golden Teacher from the first fruits I’ve grown in 15 years.
I’ll do a swab of those just to practice that technique.
There’s a nice cluster of 3 fruits that looks like the quintessential “Cubensis” so I want to clone that cluster and try and fruit a whole canopy of it.
Getting a lot of use from the laminar flow hood right from the start
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D3_Myc
Weeb Trash



Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: nogoodnamesleft]
#26981085 - 10/12/20 08:43 AM (3 years, 3 months ago) |
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I was drunk last night and decided to do some transfers. Got done, went to put them on the shelf and tripped over my damn rocking chair rocker for like the 100th time. I caught myself with the hand that was holding the fresh xfer plates. Jarred all the xfer pucks loose rolling around in their petris.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: D3_Myc] 1
#26981355 - 10/12/20 11:32 AM (3 years, 3 months ago) |
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I may have over sterilized my bags last night. Setting a timer is a great thing to do but remembering to set said timer is even better I was having nightmares about over cooking my bags when I woke up suddenly and realized I hadnt turned off the cookers. It could have been worse. Ill find out soon if its salvageable
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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nosf3r4tu


Registered: 03/26/19
Posts: 775
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: TedsDead] 1
#26981517 - 10/12/20 01:14 PM (3 years, 3 months ago) |
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ChikhaiBardo
Stranger


Registered: 12/14/19
Posts: 48
Last seen: 2 years, 11 months
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Re: Fail Thread: Share Your Fuckups and Feedback [Re: nosf3r4tu]
#26981538 - 10/12/20 01:26 PM (3 years, 3 months ago) |
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I didn’t get much response on these over at cultivation picture of the day thread. Everyone’s favorite contamination;
Tote #1




Tote #2


Edit - Yuck! And also kind of impressive looking back at it now that my anger has subsided
Edited by ChikhaiBardo (10/12/20 01:27 PM)
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