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Invisiblekrock
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Registered: 12/18/19
Posts: 154
Golden Halo, first active grow (currently colonizing oat spawn)
    #26942693 - 09/18/20 03:46 PM (3 years, 4 months ago)

Today I received a Golden Halo print from a generous shroomer!

This will be my first proper active grow.

I have tried earlier with much effort to salvage a badly stored spore syringe solution without any success. You can read about it here. Knowing that I have a legit print makes me very confident that I will be able to grow some cubes this time instead of mold.

I am also excited for the fact that I might grow some mushrooms that have golden spores.

Tomorrow I will streak some Golden Halo spores on a couple of agar plates and get this grow started. Can't wait to tomorrow :laugh:


Edited by krock (04/27/21 12:03 PM)


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OfflineOne of Us
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26942724 - 09/18/20 03:58 PM (3 years, 4 months ago)

Excellent!

Golden Halo is one of my favorites. Very aggressive mycelium, plus gold spores. I wish you the best of luck for your first successful active grow  :growshrooms:


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InvisibleJosex
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Re: Golden Halo Grow (first active grow) [Re: One of Us]
    #26942737 - 09/18/20 04:07 PM (3 years, 4 months ago)

:popcorn:


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OfflineA.k.aM
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Re: Golden Halo Grow (first active grow) [Re: Josex]
    #26942801 - 09/18/20 04:42 PM (3 years, 4 months ago)

The gold spores are still fairly rare right?

Is the potency generally normal with these? I know the myc is pretty wild.

I’m also getting ready for a first run, I’ve had it on plates for months now.


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: A.k.a]
    #26943685 - 09/19/20 08:01 AM (3 years, 4 months ago)

A day has passed and I have now streaked three agar plates!

Here is the print I received:

It's not super yellow/orange gold, but definitely golden brown.


First off, my agar plates are all normal MEA recipe. 10g Malt extract 10g agar agar and 500 ml water. I could have whipped up some softer agar, but I still have 10 plates that were ready to go, so I thought I could try germinating the spores on these plates.

I label my plates as Shimeji to avoid trouble with LE.



So for the first agar plate (A) I tried to scrape the print with my inoculation loop. I couldn't visibly discern whether I got spores on the loop or not. However I still streaked the plate properly like the tek from c10 agar guide, torching the loop between every plate rotation.





For the second plate (B) I scraped the print with a scalpel and put it on the plate. Then I streaked it like the first one. This time I am sure I got spores on it because I could see the agar went a bit darker where I put the spores with my scalpel.




On the third plate my butane torch unfortunately was almost out of juice. So instead of streaking it properly I took two scalpel samples and streaked a simple curved streak from each point and downward. Namely (C) and (D) inoculation points. The last streak I had to flame sterilize my loop with a lighter lol.




And now for some answers:



    Quote:

    Golden Halo is one of my favorites. Very aggressive mycelium, plus gold spores. I wish you the best of luck for your first successful active grow



    Thank you! I look forward to see the mycelium in action :laugh:


    Quote:

    The gold spores are still fairly rare right?



    Yep! The golden spores are not stabilized in this variety so it's like winning the lottery if you actually get golden spores.

    Quote:

    Is the potency generally normal with these? I know the myc is pretty wild.



    As far as I have read, they are the same potency as normal cubes.

    Quote:

    I’m also getting ready for a first run, I’ve had it on plates for months now.



    Cool! Feel free to share pics of your progress :laugh:



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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: krock]
    #26949095 - 09/22/20 10:33 AM (3 years, 4 months ago)

Day 3: Looks like spores on (B) plate are starting to germinate. I can see some tiny translucent mycelium clusters on the plate where I streaked. The brown part of the pic is where I put the initial spores with my scalpel.



There is also a tiny germination on the (CD) plate at the <C> inoculation point. However it was too hard to take a picture of.


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Invisiblekrock
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Registered: 12/18/19
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26950998 - 09/23/20 12:28 PM (3 years, 4 months ago)

Day 4: More germination and more growth :laugh:

(B) plate


(CD) plate


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26951030 - 09/23/20 12:56 PM (3 years, 4 months ago)

nice! I have some gh projects goin as well



What made you pick GH for your first active? It is notoriously weird and low yielding.  Still a cube tho so hard to fuck up

Any germination on the streak plate yet? Zone 1 should be showing something

The growth on those others doesnt look very clean. Later zones of the streak plate should be better tho if you grab a good lookin colony fast, even if spores are dirty af


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


Edited by c10h12n2o (09/23/20 12:58 PM)


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Invisiblekrock
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Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26951239 - 09/23/20 02:49 PM (3 years, 4 months ago)

Cool! That's some really nice looking prints!

I don't have a good reason for picking Golden Halo as my firstie, I just saw a giveaway and wanted to try it out after I failed cleaning up a extremely contaminated spore solution I had acquired. Maybe I am a bit masochistic, lol. I didn't know GH are weird/low yielding. I never seem to take the easiest route it seems :laugh:


Plate (A) where I tried to scrape spores with my loop has no germination at all. Still empty.

The streak plate where i started with scalpel scrape (B) has what looks to be many early stage germination in zone 1 along the streak line. And also one tiny germination in zone 2, however that one is kind of weird because it is located between two streak lines.

When do you usually see growth in the later zones?

Quote:

The growth on those others doesnt look very clean.



Where do you mean?


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26951286 - 09/23/20 03:12 PM (3 years, 4 months ago)

It might just be the pictures, hard to tell

But based on what im seeing i wouldn't transfer any of that. Still super early tho

When you use a loop, get it red hot then cool it in agar, then scrape spores with it. The agar on the loop will make sure it hrabs plenty of spores

If you want i can send you a print of the most forgiving and best yielding variety ive ever worked with, from a very domesticated line. Its AA+, but after about 5 years of selecting for yields and ffruit size

Even my worst tubs of it yield better than a good tub of anything else

Ive pulled 12.5 dry oz off 1 flush with a particularly good clone

Ofc the print would be ms so who knows. But its basically the opposite of GH: great yields, extremely stable, very domesticated,  gigantic fruits


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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26951627 - 09/23/20 06:25 PM (3 years, 4 months ago)

Yeah aa+ was great the couple runs I did. Plus the prints were so heavy I didn’t need a loop. There were big flakes of spores cracking off the print I just pushed one onto a plate with a blade.


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26952913 - 09/24/20 02:47 PM (3 years, 4 months ago)

With (A) plate I did torch my loop red hot, cooled it in the agar, then tried to scrape. I assume that I didn't scrape hard enough or something. Or maybe my DIY loop is not the best for this task.

I might take you up on your AA+ offer on a later time. Right now I am set on trying to grow these Golden Halos :smile:


Day 5 update:


More growth and what I assume to be mycelium is starting to become more visible.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26953029 - 09/24/20 04:16 PM (3 years, 4 months ago)

Yeah, both myc and bacteria are more visible  haha

Shoot me a dm if you ever want one of those prints, ill send it over

Looks like the print you have is pretty dirty, youll need to do some serial streakimg and/or well timed transfers to clean it up

Still no germination on plate A?

Remember it doesnt take a ton of spores,  it only takes 2 viable spores to get a dikaryotic culture

Usually when i steak spores zone 1 has visible spore streaks on the agar. If you van see any at all, its plenty (assuming they are viable and havent been microwaved or something)


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26954560 - 09/25/20 02:12 PM (3 years, 4 months ago)

Quote:

Shoot me a dm if you ever want one of those prints, ill send it over



Thank you! I appreciate it :smile:


Day 6:

Here is the (A) plate, still nothing at all. But the streak pattern is pretty visible. I cooled my loop in the middle.

I am pretty puzzled about that this plate didn't take at all as I thought you needed very little spores to get growth.


Here is (B) plate.


And a close up of zone 1 on (B) plate



I am still not quite sure what to expect from doing spores to agar. As all the actual proper mycelium I have seen are from king oyster clones I have done. And as far as I know, King Oyster mycelium looks much more like mold than cubensis mycelium does.

However I don't think there is bacteria on the plate. To me it looks more like translucent hyphae around the colonies and kind of fluffy mycelium in their center.

Of course this could be wishful thinking, but I read a while back that newly germinated spores often are translucent. I have some post quotes from my journal that I saved:

Quote:

Monokaryotic mycelium from spores will often be transparent. After a week or so you'll see it better as the strands thicken up after it becomes dikaryotic.
RR
source




Quote:

Before you see a dime's worth of growth, filamentous mycelium will already be well on its way as far out as it possibly can. You can transfer from these filaments for faster colonizers. I'm sure in the photograph provided that strands of mycelium not visible to the eye or camera are already there. Check it out with a pocket microscope if you have one they're like 10 bucks.

source





The growth on (CD) plate is pretty similar to the ones I have got on the (B) plate.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26954700 - 09/25/20 03:43 PM (3 years, 4 months ago)

Nah bro, trust me. Those cloudy discolored patches are 100% bacteria. Theres lots in those pics

You wont accidentally get monokaryotic myc. Spores clump together,  so you pretty much have to use serial dilution and/or surfactants to get that

That zone 2 patch on B might be ok, but i wouldn't transfer any of that, just because id rather have a sure thing than a "its bacterial but i still got some fruits"

Bacterial doesnt mean it wont work, it just isnt ideal

Everything is soooo much easier when you make sure you have a 100% clean culture first


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26954724 - 09/25/20 04:05 PM (3 years, 4 months ago)

Ah, you mean those brown blobs?

Those are actually just some pieces of vermiculite that stuck on the back of the plate, haha. I'll post a pic without it tomorrow :laugh:


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26954754 - 09/25/20 04:26 PM (3 years, 4 months ago)

Hahahaha yeah do that, its really hard to tell anything from the pics

Hopefully the discoloration is verm. I dont usually look for verm on agar haha


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26956092 - 09/26/20 12:56 PM (3 years, 4 months ago)

I removed the verm from the back of the plate. So here is the (B) plate. No bacteria as far as I can see :laugh:

However I still haven't figured out how to properly photograph agar. It's hard to represent how the plates actually look in real life.


Day 7:



What would you recommend transferring? The one growth in zone 2 has grown pretty big now. I don't know if it is because it has more space to grow or it is a better colonizer.


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Invisiblec10h12n2o
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Re: Golden Halo Grow (first active grow) [Re: krock]
    #26956162 - 09/26/20 01:44 PM (3 years, 4 months ago)

Ok cool, yeah that looks better

Yeah its tricky to photograph it and have it look right. Your pics are particularly tricky because it isnt focused on the colony, so even though its well organized its hard to tell if its cloudy vs whispy. Not totally convinced its axenic (only 1 organism)

You could transfer that, itll probably work, but i would rather find a better looking starting point

Maybe itll look better on a new plate

I like redundancy. If it was me i would probably transfer it and streak a few more plates. If it looks good run with it, if not, youll have lots more options to choose from by the time you find out


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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Invisiblekrock
Krockodile

Registered: 12/18/19
Posts: 154
Re: Golden Halo Grow (first active grow) [Re: c10h12n2o]
    #26956274 - 09/26/20 03:13 PM (3 years, 4 months ago)

Thanks for the input on my pictures. I always thought it was good to focus on micro surface details, to be able to see filaments or bacteria on the surface. But I agree it makes more sense to show off the shape of the colonies.
Here is a picture where I tried to focus on the colonies instead:



It does sound like a good idea to do more streak plates. I want to retry scraping spores with my loop anyway. So that I can maybe spread out the growth more than I did this time, so that it also reaches zone 3, 4 and 5. I will aim to get discoloration from the spores in zone 1 with the loop to ensure good spread. 

I am going to transfer that zone 2 growth from (B) plate and let it grow out while the new plates germinate.

When I use up the rest of my current plates (7 left) I want to try switching to some less nutritious agar as it seems to result in better looking mycelium. To this day I have always done 10g malt extract to 500 ml water. But I've seen many people on here using 7g malt extract instead.


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