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Canebrake
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To hell with petri dishes
#26952689 - 09/24/20 12:47 PM (3 years, 4 months ago) |
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I've been using little 4oz plastic serving cups for months. I decided I was going to be a "big boy" and invest in petri dishes for my agar. I liked how it was a little easier to do transfers being that they are a lower profile but that's it. Between fumbling with the lids and then having to wrap them with film. JESUS what a process. I did 25 dishes in the time it take me to do 50 of my smaller serving cups. Now I 'm stuck with a crap ton of petris.
That old saying " if it ain't broke, fix it until it is"
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dirtybirdx
getting better

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Re: To hell with petri dishes [Re: Canebrake]
#26952708 - 09/24/20 12:56 PM (3 years, 4 months ago) |
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Wrapping seemed like a nightmare at first for me too.
I chopped the Cling wrap roll into 1 inch pieces, and with some practice it goes really quickly now.
If I’m wrapping a whole sleeve, sometimes I’ll use a regular roll to wrap up the whole stack in one.
🤷🏻♂️
-------------------- Need help?

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X-Ray Cat
Sees through wooden doors

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Re: To hell with petri dishes [Re: Canebrake] 1
#26952712 - 09/24/20 12:58 PM (3 years, 4 months ago) |
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I really enjoy petri dishes but I've never tried anything else. Never will either. Wrapping them gets easier as time goes on. I was cursing up a storm the first time I used parafilm, now I love it.
--------------------
"It says number one son on the license plate. That's me. I'm the number one son." "What about me, Mom?" "You're number one son too"
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pureshrooming
feels like a stranger


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Re: To hell with petri dishes [Re: X-Ray Cat]
#26952853 - 09/24/20 02:15 PM (3 years, 4 months ago) |
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I prefer petri dishes but I've switched to cups exclusively. I constantly found myself having not ordered more and I would end up using cups anyways.
Plus doing the monthly toss of old petris into the trash always made my heart sink a little...
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verum subsequentis
seeker of truth



Registered: 03/22/16
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I love petris, Hate parafilm and Love glad cling wrap.
Op. What kind of plastic sauce cups are you using and how are you sterilizing them?
Wrapping plates becomes really really easy with a little practice.
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Gan
Wielder of Narya



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That's interesting cause in the long run petri dishes save me time and give me better visibility. So I love them compared to most no pours
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Canebrake
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I don't sterilize. I just buy them off amazon. I open them in my SAB and what I don't use I just wrap back up. If I feel they may be contaminated I rub them down with alcohol. I haven't noticed any contams in my agar doing it that way.
https://www.amazon.com/gp/product/B07THLNPRJ/ref=ppx_yo_dt_b_asin_title_o00_s00?ie=UTF8&psc=1 those are the cups
Edited by Canebrake (09/24/20 02:41 PM)
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Canebrake
Stranger
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Quote:
pureshrooming said: I prefer petri dishes but I've switched to cups exclusively. I constantly found myself having not ordered more and I would end up using cups anyways.
Plus doing the monthly toss of old petris into the trash always made my heart sink a little...
They are expensive. Thats what I don't like either. I poured 25 3 days ago and 4 had contams in them.
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verum subsequentis
seeker of truth



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Re: To hell with petri dishes [Re: Canebrake] 1
#26952910 - 09/24/20 02:46 PM (3 years, 4 months ago) |
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Then you fucked up your process.
So, you're telling me that you pour agar into these little plastic cups without sterilizing and don't see a lot of contams? Do they come wrapped and you treat cautiously until in a sab and unwrap?
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Glass petri dishes for the win. Save a turtle.
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verum subsequentis
seeker of truth



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Re: To hell with petri dishes [Re: LotKid] 1
#26952936 - 09/24/20 03:06 PM (3 years, 4 months ago) |
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Ride a cowboy
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alaskappalachian
Entitiologist

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Loc: The 49th Dimension
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I see all kinds of people doing this with sauce cups now. Even people with major cult operations. I don't get it.  I would use petris again if it wasn't 100% free for me to rotate dozens of 4oz prepours at a time. Wrapping totally, totally gets easier though. I don't miss petris, but never found that to be a problem. I run a dozen varieties of cubes and exotics and prepours meet all my needs sustainably, but I'll never understand how people get by with those sauce cups. I see it over and over and over.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Roger Clemency
Smile


Registered: 03/23/20
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I’ve tried a few different no pours and hated them. I guess it’s what you’re used to. Petris are simple, lid lifts right off, do your thing and plop back on. And you can see. Omg how you can see. +5 for Petri dishes and Parafilm
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Canebrake
Stranger
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Quote:
verum subsequentis said: Then you fucked up your process.
So, you're telling me that you pour agar into these little plastic cups without sterilizing and don't see a lot of contams? Do they come wrapped and you treat cautiously until in a sab and unwrap?
Yes they come wrapped in plastic bags. Then the lids and cups are in a separate bag inside the first bag. I open them in my SAB.
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Canebrake
Stranger
Registered: 05/01/20
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Quote:
Roger Clemency said: I’ve tried a few different no pours and hated them. I guess it’s what you’re used to. Petris are simple, lid lifts right off, do your thing and plop back on. And you can see. Omg how you can see. +5 for Petri dishes and Parafilm
Yes I agree you can see really well. My setup isn't no pour. I pour my agar into the cups, then snap the lid on after about 12-24 hours. No different than petris.
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Gan
Wielder of Narya



Registered: 08/26/19
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Re: To hell with petri dishes [Re: Canebrake]
#26953055 - 09/24/20 04:34 PM (3 years, 4 months ago) |
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Did you wrap all of the poured plates individually? If so, I can see why it took so long. Wrap them in stacks and will take you all of 5 minutes to wrap 50+ plates
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Quote:
Roger Clemency said: I’ve tried a few different no pours and hated them. I guess it’s what you’re used to. Petris are simple, lid lifts right off, do your thing and plop back on. And you can see. Omg how you can see. +5 for Petri dishes and Parafilm
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: To hell with petri dishes [Re: LotKid]
#26953088 - 09/24/20 04:53 PM (3 years, 4 months ago) |
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spore-ty



Registered: 01/21/16
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Re: To hell with petri dishes [Re: LotKid]
#26953095 - 09/24/20 04:59 PM (3 years, 4 months ago) |
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Quote:
LotKid said:
Quote:
Roger Clemency said: I’ve tried a few different no pours and hated them. I guess it’s what you’re used to. Petris are simple, lid lifts right off, do your thing and plop back on. And you can see. Omg how you can see. +5 for Petri dishes and Parafilm

Like for real those little dipping containers aren’t enough for you to diddly squat
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lookintolearn
Stranger becoming Strangest



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Re: To hell with petri dishes [Re: spore-ty]
#26953227 - 09/24/20 06:29 PM (3 years, 4 months ago) |
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To be honest I haven't made the jump to petri dishes quite yet. I have like 30 pasty plates and recently bought a whole bunch of the Alien's Holy Grail plates. I eventually would like to start using petri's but my contam rate with the Holy Grail plates is about 3-5% so far and I can make quite a few at a time.
That being said, I do hate cleaning no pours and it is a process to make them up and PC them which does get old pretty quick.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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smalltalk_canceled
Babnik


Registered: 07/13/20
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yeah FUCK petri dishes
if they werent so popular, and industry standard,
we'd use less plastic, and my unmodded jar style would be more popular.
Fuck petri dishes, fuck wrapping nice collections, no DIY style
caveman boys roll forever
-------------------- Willpower is the one true virtue
  
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Hikeadellic
Fungi Fan



Registered: 08/31/20
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I read somewhere that you could not reuse plastic petri dishes because you could never get them sterile again without melting. I have seen people use those plastic takeout sauce cups but could not figure out how to prepare them. Would alcohol be sufficient?
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
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Re: To hell with petri dishes [Re: Hikeadellic]
#26953306 - 09/24/20 07:03 PM (3 years, 4 months ago) |
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Sir Pentinite
Stranger all the time.

Registered: 05/15/19
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Re: To hell with petri dishes [Re: Hikeadellic]
#26953324 - 09/24/20 07:11 PM (3 years, 4 months ago) |
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Alcohol is not sufficient. It doesn't sterilize.
Regular petris are polystyrene and likely radiation sterilized because they will not tolerate even boiling water, let alone steam sterilization. The takeout cups are polypropylene, which will.
On a side note, styrene petris are recyclable as #6 plastic, same as disposable utensils and CD jewel cases. There's nothing to stop anyone from recycling them except paranoia.
-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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smalltalk_canceled
Babnik


Registered: 07/13/20
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people do petris because they look nice. im not a sentimental guy, what works is good, dont need pink, glitter or fairy wings to make purchase
-------------------- Willpower is the one true virtue
  
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
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i like no pour rich folks use petris
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
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Well I'd say people do petri dishes more often because they are easier visablity. However, with Holy Grail dishes the visibility is great for me. Pastyplates not so much but those are the bee's knees when it comes to plopping an agar plate into some LI or jars.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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i fuck w that glass jars im just having to replace my jars after years of use
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lookintolearn
Stranger becoming Strangest



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Re: To hell with petri dishes [Re: tripdawg420]
#26953350 - 09/24/20 07:21 PM (3 years, 4 months ago) |
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Glass jars seem a little tall for me to use agar in. I've seen people doing that and it does look good but seems like more work because you can't put alot in PC. Plus doesn't that take up a shit ton of space on work desk?
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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CocaineBuffet
Stranger



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Re: To hell with petri dishes [Re: tripdawg420]
#26953356 - 09/24/20 07:23 PM (3 years, 4 months ago) |
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When it comes to petris it is cheaper than no pours but its up to each person. I like petri dishes because they are cheap and clear but at the end of the day I wont use them more than once. Glass petri is the ideal.
Just depends on if you want to have low visibility on a pp5 container that can be reused but more expensive or a low cost pre-sterilized plastic petri.
I am admittedly in the non-eco court because I cant rationalize making the financial plunge into glass petris at this time.
As for petri dishes I have not had any issues with making them for myself and it is what I am doing currently and for the foreseeable future.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 39 minutes, 43 seconds
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u get use to it and there be times i start a grow broke af so
Edited by tripdawg420 (09/24/20 07:26 PM)
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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Re: To hell with petri dishes [Re: tripdawg420]
#26953400 - 09/24/20 07:43 PM (3 years, 4 months ago) |
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Quote:
tripdawg420 said: i like no pour rich folks use petris 
Well hott damn!!! Guess that means I finally made it!


 
All that's left to do is change my screen name to Dave Chappelle because, I'M RICH! BITCH!
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Gan
Wielder of Narya



Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
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Quote:
smalltalk_canceled said: people do petris because they look nice. im not a sentimental guy, what works is good, dont need pink, glitter or fairy wings to make purchase
Nah people (myself included) use petris cause they take less time than no pours, better visibility, easier to stack and take up less space, easier to make transfers and work with, lower sides, etc.
I use petris for everything, but no pours are great as well. I find myself switching between the two sometimes. Depending on the application. They're not just for show or flash. Or whatever nonsensical point you're attempting to make here lol
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
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Re: To hell with petri dishes [Re: Gan]
#26953423 - 09/24/20 07:54 PM (3 years, 4 months ago) |
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mfin ballers
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smalltalk_canceled
Babnik


Registered: 07/13/20
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Re: To hell with petri dishes [Re: tripdawg420]
#26953439 - 09/24/20 08:11 PM (3 years, 4 months ago) |
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see now the rich folks here come here toting flowhoods and their petri dishe they cant tolerate the poor guys having a party 3-4 posts of glass no pours - they come here to pile on their hate and show off their pp5, pp6, pp e-dicks
-------------------- Willpower is the one true virtue
  
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Mycolorado
Hobbyist


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Once you grow up you might be able to afford some petris. Prolly won’t make the bigs though.
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lookintolearn
Stranger becoming Strangest



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Re: To hell with petri dishes [Re: Mycolorado]
#26953459 - 09/24/20 08:26 PM (3 years, 4 months ago) |
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Gawd damn Mycolorado that flowhood is enormous! and is that a giant industrial pressure cooker?? I'm drooling lol
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 39 minutes, 43 seconds
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some people dont do this shit for fun but ya i need a flow hood
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Petri porn. A good shot of the halo
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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I’m just trolling with the sterilizer...it’s a $30K unit my boy offered to me for free but I had no place to put it. Really wanted to take it for a future farm. The hood is mine though and I gotta say, if you ever get the chance to use a large hood, I highly recommend it; It’s choice.
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Re: To hell with petri dishes [Re: Mycolorado] 1
#26953559 - 09/24/20 10:11 PM (3 years, 4 months ago) |
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I’ve worked with all equipment described in this post from pasty plates ( I never made nor worked with rather showed one how to do transfers for their personal agenda) for a beginner i would recommend a set of dishes which is $65-$100 for 500, make a Sab that’s hopefully $5 if you’ve got some tools or someone you know who has tools, and bam guarantee you, your contam rate will go down as long as your making good sterile procedure follow bods tek or something
You simply need the right tools and know how’s to progress on
And yeaaaaaa first flowhood was a 12x24 Now running a 24x48 so much better It’s choice
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CocaineBuffet
Stranger



Registered: 08/29/19
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Quote:
smalltalk_canceled said: see now the rich folks here come here toting flowhoods and their petri dishe they cant tolerate the poor guys having a party 3-4 posts of glass no pours - they come here to pile on their hate and show off their pp5, pp6, pp e-dicks
I pour my agar in a SAB though. I sterilize the media in a pressure cooker for 20 minutes. No flowhood needed.
When you are buying petris in bulk its literally pennies per dish.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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The cell treat dishes i love come to 5 cents (roughly) a dish when order by the box.
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LotKid
Never.Trust.A.Prankster


Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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I bet i can whittle the cost of my borosilicate plates down to the point that they've not only paid for themselves but are actually turning a profit based on cost per use analysis. And I'm saving turtles.
Investing vs spending...
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Re: To hell with petri dishes [Re: LotKid] 2
#26955086 - 09/25/20 08:44 PM (3 years, 4 months ago) |
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I'm sure. I actually did the math on yours a few weeks ago. It's obviously a good idea. Although i didn't calculate the cost of energy involved in sterilizing them. But then again, I didn't calculate in the amazing value of each turtle saved. But then again, again, I didn't calculate in the cost of each turtle fart and breath that exacerbates global warming and there by kills off penguins there by neutralizing global warming. Hmmmmm. Time for a beer.
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Lectric
Stranger

Registered: 08/31/20
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Re: To hell with petri dishes [Re: spore-ty]
#27282063 - 04/25/21 11:12 PM (2 years, 8 months ago) |
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Quote:
spore-ty said: I’ve worked with all equipment described in this post from pasty plates ( I never made nor worked with rather showed one how to do transfers for their personal agenda) for a beginner i would recommend a set of dishes which is $65-$100 for 500, make a Sab that’s hopefully $5 if you’ve got some tools or someone you know who has tools, and bam guarantee you, your contam rate will go down as long as your making good sterile procedure follow bods tek or something
You simply need the right tools and know how’s to progress on
And yeaaaaaa first flowhood was a 12x24 Now running a 24x48 so much better It’s choice
where are you guys sourcing 24x48 HEPAs?
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Hikeadellic
Fungi Fan



Registered: 08/31/20
Posts: 1,227
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Re: To hell with petri dishes [Re: Lectric]
#27285637 - 04/28/21 07:57 PM (2 years, 8 months ago) |
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I don't know about that exact size but I've heard of people finding deals on places like ebay and craigslist
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Mycelium Juice
Here for the weed



Registered: 10/31/20
Posts: 505
Loc: planet earth
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Re: To hell with petri dishes [Re: Lectric]
#27285816 - 04/28/21 10:39 PM (2 years, 8 months ago) |
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Quote:
Lectric said:
where are you guys sourcing 24x48 HEPAs?
I did a shit ton of research on that size last week. The best place I could find for 99.99% filtration was spycor @ $500 including, tax & shipping(to me). It was a 24x48x6. Make sure to find the shipping price when shopping, as some sites add $200 or more. Spycor was like $40.
Would be super interested if you find a better deal.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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48x18 is an excellent size if you want to search that size as well. My hood is this size and I really see no need to be a full 2 feet high. Even G2G with bags is not an issue.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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Mycelium Juice
Here for the weed



Registered: 10/31/20
Posts: 505
Loc: planet earth
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Re: To hell with petri dishes [Re: Smartattack]
#27285850 - 04/28/21 11:15 PM (2 years, 8 months ago) |
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Nice. That's definitely good to know. Appreciate the info Smart!
Edited by Mycelium Juice (04/28/21 11:30 PM)
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Lectric
Stranger

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Re: To hell with petri dishes [Re: Smartattack]
#27287375 - 04/30/21 01:32 AM (2 years, 8 months ago) |
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Quote:
Smartattack said: 48x18 is an excellent size if you want to search that size as well. My hood is this size and I really see no need to be a full 2 feet high. Even G2G with bags is not an issue.
How thick is the filter you use? I'm under the impression these HEPA's need to be 5-6" width?
Anyone using like 2" width HEPAs for laminar flow hood? I want to make sure i can't use these before i pay 200+ because i've found 16x18x2 HEPAs for 70 bucks..
Oh yeah, btw Smart that APE 'globe' pic is SICK
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Mycelium Juice
Here for the weed



Registered: 10/31/20
Posts: 505
Loc: planet earth
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Re: To hell with petri dishes [Re: Lectric]
#27288508 - 04/30/21 11:39 PM (2 years, 8 months ago) |
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I could be wrong here, but I believe the filter depth will indicate how long it will last. i.e. a 2 inch filter will have a shorter life span than a 6 inch filter. i'm pretty sure i'm right, but I could be wrong too
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Lectric
Stranger

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as i now understand, 6"+ width filters are necessary for laminar flow.
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: To hell with petri dishes [Re: Lectric]
#27294456 - 05/04/21 06:26 PM (2 years, 8 months ago) |
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Quote:
Lectric said:
Quote:
Smartattack said: 48x18 is an excellent size if you want to search that size as well. My hood is this size and I really see no need to be a full 2 feet high. Even G2G with bags is not an issue.
How thick is the filter you use? I'm under the impression these HEPA's need to be 5-6" width?
Anyone using like 2" width HEPAs for laminar flow hood? I want to make sure i can't use these before i pay 200+ because i've found 16x18x2 HEPAs for 70 bucks..
Oh yeah, btw Smart that APE 'globe' pic is SICK 
Whats the efficiency rating of said filter? Mine I think are 3" thick and are 99.999% ( three digits ) and I know people have used similar filters for many years. If you properly per-filter your filter you should basically never really worry about the life of the thing.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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