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InvisibleWrightii
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Registered: 05/15/20
Posts: 216
Question about slants and their genetics
    #26952651 - 09/24/20 12:28 PM (3 years, 4 months ago)

Hey people,

I have a question about mushroom strains/cultures. Hope it sounds
like I'm making some sense.

It's about making culture slants. Let's say I pick a nice mushroom from a flush,
clone it (sample from the same region but on 2 dishes) and find that this
"strain" gives me nice even flushes (using one of the 2 dishes completely).
If I would transfer some of this mycelium (lets say 0.5 cm^2 agar, from my second dish) to a slant, would I not lose much of my favourable genetics? After all I'm technically just making an isolate from a part of the agarplate I'm using.
I'm assuming I'm not dealing with a mono-strain here (which is fair right?).
Example:

How would I make an isolate of this plate that contains the same genetics as the clone it started out from? I will always be selecting some part and thus decrease diversity right?
Hope this was understandable, would love to know more!

Cheers,
-Wii


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