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Amazon Shop for: ½ Pint Jars, Agar

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Offlinedoc34
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Two Strains,One Jar
    #2695074 - 05/18/04 07:47 AM (12 years, 6 months ago)

What happens when the two are combined together?


edit=Using karo/honey water instead of multispore?


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Edited by doc34 (05/18/04 07:51 AM)


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Offlinestara
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Registered: 05/14/03
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Re: Two Strains,One Jar [Re: doc34]
    #2695151 - 05/18/04 08:53 AM (12 years, 6 months ago)

What happened if one world's given  to the whole mankind?
Americans went to the moon. :wink:
Peace :laugh:


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OfflineDr. Greenthumb
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Re: Two Strains,One Jar [Re: doc34]
    #2695180 - 05/18/04 09:10 AM (12 years, 6 months ago)



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Offlinedoc34
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Re: Two Strains,One Jar [Re: Dr. Greenthumb]
    #2695183 - 05/18/04 09:18 AM (12 years, 6 months ago)

SO in other words,you would either have one or the other strains right?
and if by chance they did mate ,you would have a new strain?

Or a Hybrid?


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Edited by doc34 (05/18/04 09:45 AM)


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Invisibleshamantra
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Registered: 05/18/04
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Re: Two Strains,One Jar [Re: doc34]
    #2695221 - 05/18/04 09:55 AM (12 years, 6 months ago)

Mixing two strains in a jar will not make a hybrid. mixing P. cubensis and P. mexicana will make a hybrid (but good luck with that).


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note: english is my 3rd languange, please ignore misspelling and poor english, im doing my best :smile:

http://youtube.com/watch?v=ug98TKkWKy0


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Anonymous

Re: Two Strains,One Jar [Re: shamantra]
    #2698117 - 05/18/04 08:27 PM (12 years, 6 months ago)

Since in the Fungal world species do not interbreed, conventionally. Hybridization is between different strains of the same species.

Mating with a single strain would be inbreeding, mating between strains would be interbreeding. The later we call hybridization, for lack of a better word.

Breeding a strain that has compatable loci of A1B1 and A2B2 with a strain that has compatable loci of A3B3 and A4 B4 would result in 100% compatability and they would all be hybrid spores produced. Many of the matings would result in lethal combinations and maybe only A1B1 and A3B3 would make fertile dikaryons. But the resulting dikaryons are hybrids. You put a A1B1 haploid nuclei under the same roof as a A3B3 nuclei. Something that did not exist before.


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Anonymous

Re: Two Strains,One Jar [Re: doc34]
    #2698153 - 05/18/04 08:34 PM (12 years, 6 months ago)

karo/honey water is still a multispore germination if spores were used to inoculate the solution. If mycelium was used and it is just liquid culture, you probably have only one dikaryon in there, or two if you inoculated with two different dikaryons from different strains.

What is important is the isolation of individual monokaryons for mating with other individula monokaryons.

Not to confuse you, but Monokaryons can also mate with Dikaryons. so you could take your favorite clone, and put that in the solution and add a monokaryon from another strain, and maybe you get lucky. But then there is still the lack of control. Is this new strain really a new strain or is it just the original dikaryon. You have to know your strains to try in do this half assed.

The most control will be monokaryon to monokaryon on agar.


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Anonymous

Re: Two Strains,One Jar [Re: shamantra]
    #2698168 - 05/18/04 08:37 PM (12 years, 6 months ago)

Interspecific, intraspecific hybrids.


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Offlinedoc34
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Re: Two Strains,One Jar [Re: ]
    #2698450 - 05/18/04 09:55 PM (12 years, 6 months ago)

"Not to confuse you, but,"

Ok,aint that the funniest shit you ever heard?

I'm trying my best to keep up Teonan ,but,I think my mind needs a memory upgrade!

The info will be studied.

Can I schedule a rematch in a month? :laugh:

Thanks again!
:cool: :thumbup:


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InvisibleHallucinogen
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Registered: 04/14/04
Posts: 1,342
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Re: Two Strains,One Jar [Re: doc34]
    #2698500 - 05/18/04 10:08 PM (12 years, 6 months ago)

Quote:

doc34 said:
and if by chance they did mate ,you would have a new strain?






LOL, your dumb :rolleyes:


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Offlinedoc34
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Re: Two Strains,One Jar [Re: Hallucinogen]
    #2698534 - 05/18/04 10:16 PM (12 years, 6 months ago)

Redneck? :smile:

Know whats even funnier?
So were you when you first started!! :shocked:

:rolleyes:


You couldnt have gotten a more mezmerising(sp?)Avatar.Hypnotic! :blush:


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InvisibleHallucinogen
Raoul Duke

Registered: 04/14/04
Posts: 1,342
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Re: Two Strains,One Jar [Re: doc34]
    #2701977 - 05/19/04 05:12 PM (12 years, 6 months ago)

Quote:

doc34 said:
Redneck? :smile:

Know whats even funnier?
So were you when you first started!! :shocked:

:rolleyes:


You couldnt have gotten a more mezmerising(sp?)Avatar.Hypnotic! :blush:




What in the blue hell are you talking about.


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Amazon Shop for: ½ Pint Jars, Agar

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