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Kauai969
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Registered: 06/08/20
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Clones on agar contam?
#26950448 - 09/23/20 03:45 AM (3 years, 4 months ago) |
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Took a dive into the world of agar, happened upon some monster 25 to 35 gram Golden Teacher fruits so I wanted to isolate it, I took spore prints which are doing fine but my clones have some odd things going on. I know what some of the common contams look like and can identify them but I wasn't sure about this. Just need some guidance. First pic is 24 hours ago other two are current
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Pearl
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Re: Clones on agar contam? [Re: Kauai969]
#26950667 - 09/23/20 08:29 AM (3 years, 4 months ago) |
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How long ago were these made?
Looks like you got some bacterial hitchhikers there...
It's hard to tell but I think I see some jump off from the fruit where you could take a transfer from 7-8 oclock on pic 3.
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diegosf
scientific dreamer



Registered: 06/30/20
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Re: Clones on agar contam? [Re: Pearl]
#26950691 - 09/23/20 08:50 AM (3 years, 4 months ago) |
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These are like air bubbles in the agar or is it like drops of water on top of it? If the second, probably bacteria.. i always have this kinda problems when taking clones, it stays until 2nd or even 4th transfer
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seand04
It is what it is



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Re: Clones on agar contam? [Re: diegosf] 1
#26950724 - 09/23/20 09:06 AM (3 years, 4 months ago) |
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just transfer the clean mycelium away to a fresh plate. It will be fine
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Kauai969
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Re: Clones on agar contam? [Re: diegosf]
#26950897 - 09/23/20 11:09 AM (3 years, 4 months ago) |
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Its water drops on top the agar, turning more to a white color
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Pearl
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Re: Clones on agar contam? [Re: Kauai969]
#26950914 - 09/23/20 11:16 AM (3 years, 4 months ago) |
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And why do you think they're turning into a white color....?
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Kauai969
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Re: Clones on agar contam? [Re: Pearl]
#26950932 - 09/23/20 11:41 AM (3 years, 4 months ago) |
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Well i assumed when it started to turn color that I was contam I just wanted to be sure I've never seen this before
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Pearl
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Re: Clones on agar contam? [Re: Kauai969]
#26950939 - 09/23/20 11:48 AM (3 years, 4 months ago) |
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Ah sorry, I misread something. Long day at work. 
Yep, water carries contams very well or I suppose 'transports' is a better term.
I'd see what it's like tomorrow (if there's no much jump off right now) and transfer from that 7-8 oclock region.
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Kauai969
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Re: Clones on agar contam? [Re: Pearl]
#26950950 - 09/23/20 11:55 AM (3 years, 4 months ago) |
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Yes, I'm used to seeing cobweb or green mold, just not this. I will check back in and see if i can work with this, im not attached to these clone plates because the spore prints i took are working great and this is just an experimental run to see if agar work is for me but still fun to see if i can save it. Thank you!
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Roger Clemency
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Registered: 03/23/20
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Re: Clones on agar contam? [Re: Kauai969]
#26951099 - 09/23/20 01:33 PM (3 years, 4 months ago) |
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Agar plates are never really considered “contaminated”. They’re meant for dealing with just this type of thing, you just cut a little wedge of clean mycelium and transfer to a new plate.
Those little spots of bacteria could’ve fallen from your hand/wrist area if you hovered or they could’ve been on the outside of the tissue you cloned if it rolled around, couldve shaken loose from somewhere if you bumped your SAB while working etc.
If you grow mushrooms, agar work is for you. It is step numero uno.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Kauai969
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Thats the mentality I'm trying to get into, went from buying pre sterilized grain and spores online to making my own everything and starting with agar, everything im currently working with is just new to me. Everything I've ever read about starting with it tells me itll put me into the "next level" I just really want it to be successful and hope I dont get ruined by the many fails I know I'm going to have.
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