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Offlineseand04
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sterile swab question
    #26949339 - 09/22/20 01:09 PM (3 years, 4 months ago)

I made some agar plates and I used sterile swabs. I used one swab full of spores from a print to make a z pattern on 4 plates each swab. Will this effect the growth. I know that spores are microscopic, so I figured a swab full of them could do 4 plates easy. I hope I didnt mess it up. First time swabbin. Hahahaha


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Offlinetripdawg420
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Re: sterile swab question [Re: seand04]
    #26949416 - 09/22/20 02:01 PM (3 years, 4 months ago)

:shrug: few days ur know


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OfflineOne of Us
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Re: sterile swab question [Re: seand04]
    #26949420 - 09/22/20 02:04 PM (3 years, 4 months ago)

That's perfectly fine.  I use at least 3-4 plates per swab (up ten if its my only swab, and I really wanna grow it).  You'll see that swabbing multiple plates works as sort of serial dilution with less colonies in later plates.  This is great for transferring away from contams, because you get distinct colonies rather than a dogpile to sort through.

Additionally, I like to stab the tip of the swab into the last plate as a coup de gras. Sometimes the spores are really imbedded in the swab, and this will be the only plate that germinates. Some pull off pieces of cotton from the swab and put that on multiple plates to achieve the same effect without putting all their eggs in one basket, so to speak.


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InvisibleRoger Clemency
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Re: sterile swab question [Re: tripdawg420]
    #26949423 - 09/22/20 02:06 PM (3 years, 4 months ago)

You kind of did serial dilution (but not really) over 4 plates instead of 1. The later plates will have fewer spores and so will have more limited genetics. That could be good, bad or indifferent.


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Offlineseand04
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Re: sterile swab question [Re: Roger Clemency]
    #26949749 - 09/22/20 04:50 PM (3 years, 4 months ago)

Awesome thanks everyone.  I was thinking about that spores being microscopic and all. Some of the plates the agar came up a lil but not too much. I used a z pattern. I don't know what serial dilution is but I am going to look it up now. Thanks again you guys


Edited by seand04 (09/22/20 04:51 PM)


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